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An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis

Sartoria hedysaroides Boiss and Heldr. (Fabaceae) is an endemic plant of Turkey that has received little scientific consideration so far. In the present study, the chemical profiles of extracts from the aerial part and roots of S. hedysaroides obtained using solvents with different polarities were a...

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Autores principales: Dall’Acqua, Stefano, Sut, Stefania, Sinan, Kouadio Ibrahime, Zengin, Gokhan, Ferrarese, Irene, Peron, Gregorio, Yildiztugay, Evren, Picot-Allain, Carene, Mahomoodally, Mohamad Fawzi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773218/
https://www.ncbi.nlm.nih.gov/pubmed/35052614
http://dx.doi.org/10.3390/antiox11010110
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author Dall’Acqua, Stefano
Sut, Stefania
Sinan, Kouadio Ibrahime
Zengin, Gokhan
Ferrarese, Irene
Peron, Gregorio
Yildiztugay, Evren
Picot-Allain, Carene
Mahomoodally, Mohamad Fawzi
author_facet Dall’Acqua, Stefano
Sut, Stefania
Sinan, Kouadio Ibrahime
Zengin, Gokhan
Ferrarese, Irene
Peron, Gregorio
Yildiztugay, Evren
Picot-Allain, Carene
Mahomoodally, Mohamad Fawzi
author_sort Dall’Acqua, Stefano
collection PubMed
description Sartoria hedysaroides Boiss and Heldr. (Fabaceae) is an endemic plant of Turkey that has received little scientific consideration so far. In the present study, the chemical profiles of extracts from the aerial part and roots of S. hedysaroides obtained using solvents with different polarities were analyzed combining integrated NMR, LC-DAD-MS(n), and LC-QTOF methods. In vitro antioxidant and enzyme inhibitory activities were evaluated, and the results were combined with chemical data using multivariate approaches. Phenolic acids, flavonoids, ellagitannins, and coumarins were identified and quantified in the extracts of aerial part and roots. Methanolic extract of S. hedysaroides aerial part showed the highest phenolic content and the highest antioxidant activity and cupric ion reducing antioxidant capacity. Dichloromethane extract of S. hedysaroides roots showed the highest inhibition of butyryl cholinesterase, while methanolic extract of S. hedysaroides aerial part was the most active tyrosinase inhibitor. Multivariate data analysis allowed us to observe a good correlation between phenolic compounds, especially caffeoylquinic derivatives and flavonoids and the antioxidant activity of extracts. Acetylcholinesterase inhibition was correlated with the presence of caffeoylquinic acids and coumarins. Overall, the present study appraised the biological potential of understudied S. hedysaroides, and provided a comprehensive approach combining metabolomic characterization of plant material and multivariate data analysis for the correlation of chemical data with results from multi-target biological assays.
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spelling pubmed-87732182022-01-21 An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis Dall’Acqua, Stefano Sut, Stefania Sinan, Kouadio Ibrahime Zengin, Gokhan Ferrarese, Irene Peron, Gregorio Yildiztugay, Evren Picot-Allain, Carene Mahomoodally, Mohamad Fawzi Antioxidants (Basel) Article Sartoria hedysaroides Boiss and Heldr. (Fabaceae) is an endemic plant of Turkey that has received little scientific consideration so far. In the present study, the chemical profiles of extracts from the aerial part and roots of S. hedysaroides obtained using solvents with different polarities were analyzed combining integrated NMR, LC-DAD-MS(n), and LC-QTOF methods. In vitro antioxidant and enzyme inhibitory activities were evaluated, and the results were combined with chemical data using multivariate approaches. Phenolic acids, flavonoids, ellagitannins, and coumarins were identified and quantified in the extracts of aerial part and roots. Methanolic extract of S. hedysaroides aerial part showed the highest phenolic content and the highest antioxidant activity and cupric ion reducing antioxidant capacity. Dichloromethane extract of S. hedysaroides roots showed the highest inhibition of butyryl cholinesterase, while methanolic extract of S. hedysaroides aerial part was the most active tyrosinase inhibitor. Multivariate data analysis allowed us to observe a good correlation between phenolic compounds, especially caffeoylquinic derivatives and flavonoids and the antioxidant activity of extracts. Acetylcholinesterase inhibition was correlated with the presence of caffeoylquinic acids and coumarins. Overall, the present study appraised the biological potential of understudied S. hedysaroides, and provided a comprehensive approach combining metabolomic characterization of plant material and multivariate data analysis for the correlation of chemical data with results from multi-target biological assays. MDPI 2022-01-04 /pmc/articles/PMC8773218/ /pubmed/35052614 http://dx.doi.org/10.3390/antiox11010110 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dall’Acqua, Stefano
Sut, Stefania
Sinan, Kouadio Ibrahime
Zengin, Gokhan
Ferrarese, Irene
Peron, Gregorio
Yildiztugay, Evren
Picot-Allain, Carene
Mahomoodally, Mohamad Fawzi
An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
title An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
title_full An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
title_fullStr An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
title_full_unstemmed An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
title_short An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
title_sort integrated nmr, lc-dad-ms, lc-qtof metabolomic characterization of sartoria hedysaroides: correlation of antioxidant and enzyme inhibitory activity with chemical composition by multivariate data analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773218/
https://www.ncbi.nlm.nih.gov/pubmed/35052614
http://dx.doi.org/10.3390/antiox11010110
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