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Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization

Staphylococcus aureus (S. aureus) infections, particularly methicillin-resistant Staphylococcus aureus (MRSA) in humans and animals, have become a significant concern globally. The present study aimed to determine the prevalence and antibiogram of S. aureus isolated from animal handlers in Peninsula...

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Autores principales: Chai, Minhian, Sukiman, Muhammad Zikree, Kamarun Baharin, Amirah Huda, Ramlan, Insyirah, Lai, Lennard Zhunhoong, Liew, Yeewen, Malayandy, Pavitra, Mohamad, Noor Muzamil, Choong, Siewshean, Ariffin, Siti Mariam Zainal, Ghazali, Mohd Faizal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773339/
https://www.ncbi.nlm.nih.gov/pubmed/35052980
http://dx.doi.org/10.3390/antibiotics11010103
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author Chai, Minhian
Sukiman, Muhammad Zikree
Kamarun Baharin, Amirah Huda
Ramlan, Insyirah
Lai, Lennard Zhunhoong
Liew, Yeewen
Malayandy, Pavitra
Mohamad, Noor Muzamil
Choong, Siewshean
Ariffin, Siti Mariam Zainal
Ghazali, Mohd Faizal
author_facet Chai, Minhian
Sukiman, Muhammad Zikree
Kamarun Baharin, Amirah Huda
Ramlan, Insyirah
Lai, Lennard Zhunhoong
Liew, Yeewen
Malayandy, Pavitra
Mohamad, Noor Muzamil
Choong, Siewshean
Ariffin, Siti Mariam Zainal
Ghazali, Mohd Faizal
author_sort Chai, Minhian
collection PubMed
description Staphylococcus aureus (S. aureus) infections, particularly methicillin-resistant Staphylococcus aureus (MRSA) in humans and animals, have become a significant concern globally. The present study aimed to determine the prevalence and antibiogram of S. aureus isolated from animal handlers in Peninsular Malaysia. Furthermore, the genotypic characteristics of S. aureus isolates were also investigated. Nasal and oral swab samples were collected from 423 animal handlers in Peninsular Malaysia. The antibiogram profiles of S. aureus against 18 antibiotics were established using a Kirby–Bauer test. The genotypic profile of S. aureus, including the presence of antimicrobial resistance (AMR), virulence genes and spa genotypes, was investigated using molecular techniques. The overall carriage rate of S. aureus, MRSA and MDRSA was 30.5%, 1.2% and 19.4%, respectively. S. aureus was highly resistant against penicillin (72.3%) and amoxicillin (52.3%). Meanwhile, gentamicin and linezolid were fully effective against all the isolated S. aureus from animal handlers. It was observed that animal handlers with close exposure to poultry were more likely to carry S. aureus that is resistant to tetracycline and erythromycin. S. aureus isolates harboured tetracycline resistance (tetK, tetL and tetM), erythromycin resistance (ermA, ermB, ermC and msrA) and immune evasion cluster (IEC) genes (scn, chp, sak, sea and sep). Seventeen different spa types were detected among the 30 isolates of MDRSA, with t189 (16.7%) and t4171 (16.7%) being the predominant spa type, suggesting wide genetic diversity of the MDRSA isolates. The present study demonstrated the prevalence of S. aureus strains, including MRSA and MDRSA with various antimicrobial resistance and genetic profiles from animal handlers in Peninsular Malaysia.
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spelling pubmed-87733392022-01-21 Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization Chai, Minhian Sukiman, Muhammad Zikree Kamarun Baharin, Amirah Huda Ramlan, Insyirah Lai, Lennard Zhunhoong Liew, Yeewen Malayandy, Pavitra Mohamad, Noor Muzamil Choong, Siewshean Ariffin, Siti Mariam Zainal Ghazali, Mohd Faizal Antibiotics (Basel) Article Staphylococcus aureus (S. aureus) infections, particularly methicillin-resistant Staphylococcus aureus (MRSA) in humans and animals, have become a significant concern globally. The present study aimed to determine the prevalence and antibiogram of S. aureus isolated from animal handlers in Peninsular Malaysia. Furthermore, the genotypic characteristics of S. aureus isolates were also investigated. Nasal and oral swab samples were collected from 423 animal handlers in Peninsular Malaysia. The antibiogram profiles of S. aureus against 18 antibiotics were established using a Kirby–Bauer test. The genotypic profile of S. aureus, including the presence of antimicrobial resistance (AMR), virulence genes and spa genotypes, was investigated using molecular techniques. The overall carriage rate of S. aureus, MRSA and MDRSA was 30.5%, 1.2% and 19.4%, respectively. S. aureus was highly resistant against penicillin (72.3%) and amoxicillin (52.3%). Meanwhile, gentamicin and linezolid were fully effective against all the isolated S. aureus from animal handlers. It was observed that animal handlers with close exposure to poultry were more likely to carry S. aureus that is resistant to tetracycline and erythromycin. S. aureus isolates harboured tetracycline resistance (tetK, tetL and tetM), erythromycin resistance (ermA, ermB, ermC and msrA) and immune evasion cluster (IEC) genes (scn, chp, sak, sea and sep). Seventeen different spa types were detected among the 30 isolates of MDRSA, with t189 (16.7%) and t4171 (16.7%) being the predominant spa type, suggesting wide genetic diversity of the MDRSA isolates. The present study demonstrated the prevalence of S. aureus strains, including MRSA and MDRSA with various antimicrobial resistance and genetic profiles from animal handlers in Peninsular Malaysia. MDPI 2022-01-14 /pmc/articles/PMC8773339/ /pubmed/35052980 http://dx.doi.org/10.3390/antibiotics11010103 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chai, Minhian
Sukiman, Muhammad Zikree
Kamarun Baharin, Amirah Huda
Ramlan, Insyirah
Lai, Lennard Zhunhoong
Liew, Yeewen
Malayandy, Pavitra
Mohamad, Noor Muzamil
Choong, Siewshean
Ariffin, Siti Mariam Zainal
Ghazali, Mohd Faizal
Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization
title Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization
title_full Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization
title_fullStr Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization
title_full_unstemmed Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization
title_short Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization
title_sort methicillin-resistant staphylococcus aureus from peninsular malaysian animal handlers: molecular profile, antimicrobial resistance, immune evasion cluster and genotypic categorization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773339/
https://www.ncbi.nlm.nih.gov/pubmed/35052980
http://dx.doi.org/10.3390/antibiotics11010103
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