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Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress

Oxidative stress (OS) is implicated in the pathogenesis of several neurodegenerative diseases. We have previously shown that N-acyl dopamines (N-ADA and N-DDA) protect the neural cells of healthy donors and patients with Parkinson’s disease from OS. In this study, we assessed the effects of N-acyl d...

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Autores principales: Novosadova, Ekaterina, Dolotov, Oleg, Inozemtseva, Ludmila, Novosadova, Ludmila, Antonov, Stanislav, Shimchenko, Darya, Bezuglov, Vladimir, Vetchinova, Anna, Tarantul, Vyacheslav, Grivennikov, Igor, Illarioshkin, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773408/
https://www.ncbi.nlm.nih.gov/pubmed/35052646
http://dx.doi.org/10.3390/antiox11010142
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author Novosadova, Ekaterina
Dolotov, Oleg
Inozemtseva, Ludmila
Novosadova, Ludmila
Antonov, Stanislav
Shimchenko, Darya
Bezuglov, Vladimir
Vetchinova, Anna
Tarantul, Vyacheslav
Grivennikov, Igor
Illarioshkin, Sergey
author_facet Novosadova, Ekaterina
Dolotov, Oleg
Inozemtseva, Ludmila
Novosadova, Ludmila
Antonov, Stanislav
Shimchenko, Darya
Bezuglov, Vladimir
Vetchinova, Anna
Tarantul, Vyacheslav
Grivennikov, Igor
Illarioshkin, Sergey
author_sort Novosadova, Ekaterina
collection PubMed
description Oxidative stress (OS) is implicated in the pathogenesis of several neurodegenerative diseases. We have previously shown that N-acyl dopamines (N-ADA and N-DDA) protect the neural cells of healthy donors and patients with Parkinson’s disease from OS. In this study, we assessed the effects of N-acyl dopamines on the expression of neurotrophic factors in human-induced pluripotent stem cell-derived neuronal cultures enriched with dopaminergic neurons under conditions of OS induced by hydrogen peroxide. We showed that hydrogen peroxide treatment increased BDNF but not GDNF mRNA levels, while it did not affect the secretion of corresponding proteins into the culture medium of these cells. Application of N-acyl dopamines promoted BDNF release into the culture medium. Under conditions of OS, N-DDA also increased TRKB, TRKC and RET mRNA levels. Furthermore, N-acyl dopamines prevented cell death 24 h after OS induction and promoted the expression of antioxidant enzymes GPX1, GPX7, SOD1, SOD2 and CAT, as well as reduced the BAX/BCL2 mRNA ratio. These findings indicate that stimulation of the expression of neurotrophic factors and their receptors may underlie the neuroprotective effects of N-acyl dopamines in human neurons.
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spelling pubmed-87734082022-01-21 Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress Novosadova, Ekaterina Dolotov, Oleg Inozemtseva, Ludmila Novosadova, Ludmila Antonov, Stanislav Shimchenko, Darya Bezuglov, Vladimir Vetchinova, Anna Tarantul, Vyacheslav Grivennikov, Igor Illarioshkin, Sergey Antioxidants (Basel) Article Oxidative stress (OS) is implicated in the pathogenesis of several neurodegenerative diseases. We have previously shown that N-acyl dopamines (N-ADA and N-DDA) protect the neural cells of healthy donors and patients with Parkinson’s disease from OS. In this study, we assessed the effects of N-acyl dopamines on the expression of neurotrophic factors in human-induced pluripotent stem cell-derived neuronal cultures enriched with dopaminergic neurons under conditions of OS induced by hydrogen peroxide. We showed that hydrogen peroxide treatment increased BDNF but not GDNF mRNA levels, while it did not affect the secretion of corresponding proteins into the culture medium of these cells. Application of N-acyl dopamines promoted BDNF release into the culture medium. Under conditions of OS, N-DDA also increased TRKB, TRKC and RET mRNA levels. Furthermore, N-acyl dopamines prevented cell death 24 h after OS induction and promoted the expression of antioxidant enzymes GPX1, GPX7, SOD1, SOD2 and CAT, as well as reduced the BAX/BCL2 mRNA ratio. These findings indicate that stimulation of the expression of neurotrophic factors and their receptors may underlie the neuroprotective effects of N-acyl dopamines in human neurons. MDPI 2022-01-10 /pmc/articles/PMC8773408/ /pubmed/35052646 http://dx.doi.org/10.3390/antiox11010142 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Novosadova, Ekaterina
Dolotov, Oleg
Inozemtseva, Ludmila
Novosadova, Ludmila
Antonov, Stanislav
Shimchenko, Darya
Bezuglov, Vladimir
Vetchinova, Anna
Tarantul, Vyacheslav
Grivennikov, Igor
Illarioshkin, Sergey
Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
title Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
title_full Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
title_fullStr Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
title_full_unstemmed Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
title_short Influence of N-Arachidonoyl Dopamine and N-Docosahexaenoyl Dopamine on the Expression of Neurotrophic Factors in Neuronal Differentiated Cultures of Human Induced Pluripotent Stem Cells under Conditions of Oxidative Stress
title_sort influence of n-arachidonoyl dopamine and n-docosahexaenoyl dopamine on the expression of neurotrophic factors in neuronal differentiated cultures of human induced pluripotent stem cells under conditions of oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773408/
https://www.ncbi.nlm.nih.gov/pubmed/35052646
http://dx.doi.org/10.3390/antiox11010142
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