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Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses

Roses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcripto...

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Autores principales: Yi, Xingwan, Gao, Huabei, Yang, Yi, Yang, Shumin, Luo, Le, Yu, Chao, Wang, Jia, Cheng, Tangren, Zhang, Qixiang, Pan, Huitang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773502/
https://www.ncbi.nlm.nih.gov/pubmed/35053206
http://dx.doi.org/10.3390/biom12010058
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author Yi, Xingwan
Gao, Huabei
Yang, Yi
Yang, Shumin
Luo, Le
Yu, Chao
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
Pan, Huitang
author_facet Yi, Xingwan
Gao, Huabei
Yang, Yi
Yang, Shumin
Luo, Le
Yu, Chao
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
Pan, Huitang
author_sort Yi, Xingwan
collection PubMed
description Roses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcriptome data of once- and continuous-flowering roses in our previous study were used to conduct weighted gene co-expression network analysis (WGCNA) to obtain the candidate genes related to flowering transitions. The expression patterns of candidate genes at different developmental stages between Rosa chinensis “Old Blush” (continuous-flowering cultivar) and R. “Huan Die” (once-flowering cultivar) were investigated, and the relationship of the key gene with the endogenous hormone was analyzed. The results showed that the expression trends of VIN3-LIKE 1 (VIL1), FRIGIDA- LIKE 3 (FRI3), APETALA 2- LIKE (AP2-like) and CONSTANS-LIKE 2 (CO-like 2) genes were significantly different between “Old Blush” and “Huan Die”, and the expression trends of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) and CO-like 2 were consistent in the flowering transition of “Old Blush” under different environments. The changes in cytokinin and gibberellic acid (GA(3)) content were different in the two rose cultivars. The overall change trend of the abscisic acid and GA(3) in the flowering transition of “Old Blush” under different environments was consistent. The promoter sequence of CO-like 2 contained a P-box element associated with gibberellin response, as well as binding sites for transcription factors. In a word, we found CO-like 2 associated with continuous flowering and some factors that may synergistically regulate continuous flowering. The results provided a reference for elucidating the molecular regulatory mechanisms of continuous-flowering traits in roses.
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spelling pubmed-87735022022-01-21 Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses Yi, Xingwan Gao, Huabei Yang, Yi Yang, Shumin Luo, Le Yu, Chao Wang, Jia Cheng, Tangren Zhang, Qixiang Pan, Huitang Biomolecules Article Roses are the most important cut flower crops and widely used woody ornamental plants in gardens throughout the world, and they are model plants for studying the continuous-flowering trait of woody plants. To analyze the molecular regulation mechanism of continuous flowering, comparative transcriptome data of once- and continuous-flowering roses in our previous study were used to conduct weighted gene co-expression network analysis (WGCNA) to obtain the candidate genes related to flowering transitions. The expression patterns of candidate genes at different developmental stages between Rosa chinensis “Old Blush” (continuous-flowering cultivar) and R. “Huan Die” (once-flowering cultivar) were investigated, and the relationship of the key gene with the endogenous hormone was analyzed. The results showed that the expression trends of VIN3-LIKE 1 (VIL1), FRIGIDA- LIKE 3 (FRI3), APETALA 2- LIKE (AP2-like) and CONSTANS-LIKE 2 (CO-like 2) genes were significantly different between “Old Blush” and “Huan Die”, and the expression trends of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) and CO-like 2 were consistent in the flowering transition of “Old Blush” under different environments. The changes in cytokinin and gibberellic acid (GA(3)) content were different in the two rose cultivars. The overall change trend of the abscisic acid and GA(3) in the flowering transition of “Old Blush” under different environments was consistent. The promoter sequence of CO-like 2 contained a P-box element associated with gibberellin response, as well as binding sites for transcription factors. In a word, we found CO-like 2 associated with continuous flowering and some factors that may synergistically regulate continuous flowering. The results provided a reference for elucidating the molecular regulatory mechanisms of continuous-flowering traits in roses. MDPI 2021-12-31 /pmc/articles/PMC8773502/ /pubmed/35053206 http://dx.doi.org/10.3390/biom12010058 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yi, Xingwan
Gao, Huabei
Yang, Yi
Yang, Shumin
Luo, Le
Yu, Chao
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
Pan, Huitang
Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
title Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
title_full Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
title_fullStr Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
title_full_unstemmed Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
title_short Differentially Expressed Genes Related to Flowering Transition between Once- and Continuous-Flowering Roses
title_sort differentially expressed genes related to flowering transition between once- and continuous-flowering roses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773502/
https://www.ncbi.nlm.nih.gov/pubmed/35053206
http://dx.doi.org/10.3390/biom12010058
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