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Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models

SIMPLE SUMMARY: The immune system responds to abnormal cell growth by sending immune cells to kill them. The immune cell response is very important since it can usually stop abnormal cells from growing and spreading. Immuno-therapeutics used to treat cancer require help from the immune system to be...

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Autores principales: Bernhard, Wendy, Barreto, Kris, El-Sayed, Ayman, DeCoteau, John, Geyer, C. Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773629/
https://www.ncbi.nlm.nih.gov/pubmed/35053466
http://dx.doi.org/10.3390/cancers14020300
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author Bernhard, Wendy
Barreto, Kris
El-Sayed, Ayman
DeCoteau, John
Geyer, C. Ronald
author_facet Bernhard, Wendy
Barreto, Kris
El-Sayed, Ayman
DeCoteau, John
Geyer, C. Ronald
author_sort Bernhard, Wendy
collection PubMed
description SIMPLE SUMMARY: The immune system responds to abnormal cell growth by sending immune cells to kill them. The immune cell response is very important since it can usually stop abnormal cells from growing and spreading. Immuno-therapeutics used to treat cancer require help from the immune system to be effective. A biopsy is typically performed to determine the therapeutic efficacy of cancer treatment which is invasive and difficult. A simpler and less invasive way to monitor therapeutic efficacy is needed. Here, we show a molecule that can be used as an imaging agent to determine immune cell recruitment to tumors. ABSTRACT: Tracking immune responses is complex due to the mixture of cell types, variability in cell populations, and the dynamic environment. Tissue biopsies and blood analysis can identify infiltrating and circulating immune cells; however, due to the dynamic nature of the immune response, these are prone to sampling errors. Non-invasive targeted molecular imaging provides a method to monitor immune response, which has advantages of providing whole-body images, being non-invasive, and allowing longitudinal monitoring. Three non-specific Fc-containing proteins were labeled with near-infrared dye IRDye800CW and used as imaging probes to assess tumor-infiltrating immune cells in FaDu and A-431 xenograft models. We showed that Fc domains localize to tumors and are visible by fluorescent imaging. This tumor localization appears to be based on binding tumor-associated immune cells and some xenografts showed higher fluorescent signals than others. The Fc domain alone bound to different human immune cell types. The Fc domain can be a valuable research tool to study innate immune response.
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spelling pubmed-87736292022-01-21 Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models Bernhard, Wendy Barreto, Kris El-Sayed, Ayman DeCoteau, John Geyer, C. Ronald Cancers (Basel) Article SIMPLE SUMMARY: The immune system responds to abnormal cell growth by sending immune cells to kill them. The immune cell response is very important since it can usually stop abnormal cells from growing and spreading. Immuno-therapeutics used to treat cancer require help from the immune system to be effective. A biopsy is typically performed to determine the therapeutic efficacy of cancer treatment which is invasive and difficult. A simpler and less invasive way to monitor therapeutic efficacy is needed. Here, we show a molecule that can be used as an imaging agent to determine immune cell recruitment to tumors. ABSTRACT: Tracking immune responses is complex due to the mixture of cell types, variability in cell populations, and the dynamic environment. Tissue biopsies and blood analysis can identify infiltrating and circulating immune cells; however, due to the dynamic nature of the immune response, these are prone to sampling errors. Non-invasive targeted molecular imaging provides a method to monitor immune response, which has advantages of providing whole-body images, being non-invasive, and allowing longitudinal monitoring. Three non-specific Fc-containing proteins were labeled with near-infrared dye IRDye800CW and used as imaging probes to assess tumor-infiltrating immune cells in FaDu and A-431 xenograft models. We showed that Fc domains localize to tumors and are visible by fluorescent imaging. This tumor localization appears to be based on binding tumor-associated immune cells and some xenografts showed higher fluorescent signals than others. The Fc domain alone bound to different human immune cell types. The Fc domain can be a valuable research tool to study innate immune response. MDPI 2022-01-08 /pmc/articles/PMC8773629/ /pubmed/35053466 http://dx.doi.org/10.3390/cancers14020300 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bernhard, Wendy
Barreto, Kris
El-Sayed, Ayman
DeCoteau, John
Geyer, C. Ronald
Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
title Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
title_full Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
title_fullStr Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
title_full_unstemmed Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
title_short Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
title_sort imaging immune cells using fc domain probes in mouse cancer xenograft models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773629/
https://www.ncbi.nlm.nih.gov/pubmed/35053466
http://dx.doi.org/10.3390/cancers14020300
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