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Revisiting the Non-Coding Nature of Pospiviroids
Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and struc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773695/ https://www.ncbi.nlm.nih.gov/pubmed/35053381 http://dx.doi.org/10.3390/cells11020265 |
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author | Katsarou, Konstantina Adkar-Purushothama, Charith Raj Tassios, Emilios Samiotaki, Martina Andronis, Christos Lisón, Purificación Nikolaou, Christoforos Perreault, Jean-Pierre Kalantidis, Kriton |
author_facet | Katsarou, Konstantina Adkar-Purushothama, Charith Raj Tassios, Emilios Samiotaki, Martina Andronis, Christos Lisón, Purificación Nikolaou, Christoforos Perreault, Jean-Pierre Kalantidis, Kriton |
author_sort | Katsarou, Konstantina |
collection | PubMed |
description | Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and structure, that were shown to be translated (e.g., cirRNAs, precursors of miRNA, RNA satellites) as well as studies showing that some viroids are located in ribosomes, have reignited the idea that viroids may be translated. In this study, we used advanced bioinformatic analysis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide production but rather to another unknown function that requires further study. |
format | Online Article Text |
id | pubmed-8773695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87736952022-01-21 Revisiting the Non-Coding Nature of Pospiviroids Katsarou, Konstantina Adkar-Purushothama, Charith Raj Tassios, Emilios Samiotaki, Martina Andronis, Christos Lisón, Purificación Nikolaou, Christoforos Perreault, Jean-Pierre Kalantidis, Kriton Cells Article Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and structure, that were shown to be translated (e.g., cirRNAs, precursors of miRNA, RNA satellites) as well as studies showing that some viroids are located in ribosomes, have reignited the idea that viroids may be translated. In this study, we used advanced bioinformatic analysis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide production but rather to another unknown function that requires further study. MDPI 2022-01-13 /pmc/articles/PMC8773695/ /pubmed/35053381 http://dx.doi.org/10.3390/cells11020265 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Katsarou, Konstantina Adkar-Purushothama, Charith Raj Tassios, Emilios Samiotaki, Martina Andronis, Christos Lisón, Purificación Nikolaou, Christoforos Perreault, Jean-Pierre Kalantidis, Kriton Revisiting the Non-Coding Nature of Pospiviroids |
title | Revisiting the Non-Coding Nature of Pospiviroids |
title_full | Revisiting the Non-Coding Nature of Pospiviroids |
title_fullStr | Revisiting the Non-Coding Nature of Pospiviroids |
title_full_unstemmed | Revisiting the Non-Coding Nature of Pospiviroids |
title_short | Revisiting the Non-Coding Nature of Pospiviroids |
title_sort | revisiting the non-coding nature of pospiviroids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773695/ https://www.ncbi.nlm.nih.gov/pubmed/35053381 http://dx.doi.org/10.3390/cells11020265 |
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