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Revisiting the Non-Coding Nature of Pospiviroids

Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and struc...

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Autores principales: Katsarou, Konstantina, Adkar-Purushothama, Charith Raj, Tassios, Emilios, Samiotaki, Martina, Andronis, Christos, Lisón, Purificación, Nikolaou, Christoforos, Perreault, Jean-Pierre, Kalantidis, Kriton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773695/
https://www.ncbi.nlm.nih.gov/pubmed/35053381
http://dx.doi.org/10.3390/cells11020265
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author Katsarou, Konstantina
Adkar-Purushothama, Charith Raj
Tassios, Emilios
Samiotaki, Martina
Andronis, Christos
Lisón, Purificación
Nikolaou, Christoforos
Perreault, Jean-Pierre
Kalantidis, Kriton
author_facet Katsarou, Konstantina
Adkar-Purushothama, Charith Raj
Tassios, Emilios
Samiotaki, Martina
Andronis, Christos
Lisón, Purificación
Nikolaou, Christoforos
Perreault, Jean-Pierre
Kalantidis, Kriton
author_sort Katsarou, Konstantina
collection PubMed
description Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and structure, that were shown to be translated (e.g., cirRNAs, precursors of miRNA, RNA satellites) as well as studies showing that some viroids are located in ribosomes, have reignited the idea that viroids may be translated. In this study, we used advanced bioinformatic analysis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide production but rather to another unknown function that requires further study.
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spelling pubmed-87736952022-01-21 Revisiting the Non-Coding Nature of Pospiviroids Katsarou, Konstantina Adkar-Purushothama, Charith Raj Tassios, Emilios Samiotaki, Martina Andronis, Christos Lisón, Purificación Nikolaou, Christoforos Perreault, Jean-Pierre Kalantidis, Kriton Cells Article Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and structure, that were shown to be translated (e.g., cirRNAs, precursors of miRNA, RNA satellites) as well as studies showing that some viroids are located in ribosomes, have reignited the idea that viroids may be translated. In this study, we used advanced bioinformatic analysis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide production but rather to another unknown function that requires further study. MDPI 2022-01-13 /pmc/articles/PMC8773695/ /pubmed/35053381 http://dx.doi.org/10.3390/cells11020265 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Katsarou, Konstantina
Adkar-Purushothama, Charith Raj
Tassios, Emilios
Samiotaki, Martina
Andronis, Christos
Lisón, Purificación
Nikolaou, Christoforos
Perreault, Jean-Pierre
Kalantidis, Kriton
Revisiting the Non-Coding Nature of Pospiviroids
title Revisiting the Non-Coding Nature of Pospiviroids
title_full Revisiting the Non-Coding Nature of Pospiviroids
title_fullStr Revisiting the Non-Coding Nature of Pospiviroids
title_full_unstemmed Revisiting the Non-Coding Nature of Pospiviroids
title_short Revisiting the Non-Coding Nature of Pospiviroids
title_sort revisiting the non-coding nature of pospiviroids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773695/
https://www.ncbi.nlm.nih.gov/pubmed/35053381
http://dx.doi.org/10.3390/cells11020265
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