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Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)

Despite its reduced sensitivity, sputum smear microscopy (SSM) remains the main diagnostic test for detecting tuberculosis in many parts of the world. A new diagnostic technique, the magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was optimized by evaluating different concentrations...

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Autores principales: Gordillo-Marroquín, Cristina, Sánchez-Pérez, Héctor J., Gómez-Velasco, Anaximandro, Martín, Miguel, Guillén-Navarro, Karina, Vázquez-Marcelín, Janeth, Gómez-Bustamante, Adriana, Jonapá-Gómez, Letisia, Alocilja, Evangelyn C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773761/
https://www.ncbi.nlm.nih.gov/pubmed/35049656
http://dx.doi.org/10.3390/bios12010029
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author Gordillo-Marroquín, Cristina
Sánchez-Pérez, Héctor J.
Gómez-Velasco, Anaximandro
Martín, Miguel
Guillén-Navarro, Karina
Vázquez-Marcelín, Janeth
Gómez-Bustamante, Adriana
Jonapá-Gómez, Letisia
Alocilja, Evangelyn C.
author_facet Gordillo-Marroquín, Cristina
Sánchez-Pérez, Héctor J.
Gómez-Velasco, Anaximandro
Martín, Miguel
Guillén-Navarro, Karina
Vázquez-Marcelín, Janeth
Gómez-Bustamante, Adriana
Jonapá-Gómez, Letisia
Alocilja, Evangelyn C.
author_sort Gordillo-Marroquín, Cristina
collection PubMed
description Despite its reduced sensitivity, sputum smear microscopy (SSM) remains the main diagnostic test for detecting tuberculosis in many parts of the world. A new diagnostic technique, the magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was optimized by evaluating different concentrations of glycan-functionalized magnetic nanoparticles (GMNP) and Tween 80 to improve the acid-fast bacilli (AFB) count. Comparative analysis was performed on 225 sputum smears: 30 with SSM, 107 with NCBA at different GMNP concentrations, and 88 with NCBA-Tween 80 at various concentrations and incubation times. AFB quantification was performed by adding the total number of AFB in all fields per smear and classified according to standard guidelines (scanty, 1+, 2+ and 3+). Smears by NCBA with low GMNP concentrations (≤1.5 mg/mL) showed higher AFB quantification compared to SSM. Cell enrichment of sputum samples by combining NCBA-GMNP, incubated with Tween 80 (5%) for three minutes, improved capture efficiency and increased AFB detection up to 445% over SSM. NCBA with Tween 80 offers the opportunity to improve TB diagnostics, mainly in paucibacillary cases. As this method provides biosafety with a simple and inexpensive methodology that obtains results in a short time, it might be considered as a point-of-care TB diagnostic method in regions where resources are limited.
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spelling pubmed-87737612022-01-21 Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA) Gordillo-Marroquín, Cristina Sánchez-Pérez, Héctor J. Gómez-Velasco, Anaximandro Martín, Miguel Guillén-Navarro, Karina Vázquez-Marcelín, Janeth Gómez-Bustamante, Adriana Jonapá-Gómez, Letisia Alocilja, Evangelyn C. Biosensors (Basel) Article Despite its reduced sensitivity, sputum smear microscopy (SSM) remains the main diagnostic test for detecting tuberculosis in many parts of the world. A new diagnostic technique, the magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was optimized by evaluating different concentrations of glycan-functionalized magnetic nanoparticles (GMNP) and Tween 80 to improve the acid-fast bacilli (AFB) count. Comparative analysis was performed on 225 sputum smears: 30 with SSM, 107 with NCBA at different GMNP concentrations, and 88 with NCBA-Tween 80 at various concentrations and incubation times. AFB quantification was performed by adding the total number of AFB in all fields per smear and classified according to standard guidelines (scanty, 1+, 2+ and 3+). Smears by NCBA with low GMNP concentrations (≤1.5 mg/mL) showed higher AFB quantification compared to SSM. Cell enrichment of sputum samples by combining NCBA-GMNP, incubated with Tween 80 (5%) for three minutes, improved capture efficiency and increased AFB detection up to 445% over SSM. NCBA with Tween 80 offers the opportunity to improve TB diagnostics, mainly in paucibacillary cases. As this method provides biosafety with a simple and inexpensive methodology that obtains results in a short time, it might be considered as a point-of-care TB diagnostic method in regions where resources are limited. MDPI 2022-01-07 /pmc/articles/PMC8773761/ /pubmed/35049656 http://dx.doi.org/10.3390/bios12010029 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gordillo-Marroquín, Cristina
Sánchez-Pérez, Héctor J.
Gómez-Velasco, Anaximandro
Martín, Miguel
Guillén-Navarro, Karina
Vázquez-Marcelín, Janeth
Gómez-Bustamante, Adriana
Jonapá-Gómez, Letisia
Alocilja, Evangelyn C.
Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
title Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
title_full Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
title_fullStr Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
title_full_unstemmed Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
title_short Tween 80 Improves the Acid-Fast Bacilli Quantification in the Magnetic Nanoparticle-Based Colorimetric Biosensing Assay (NCBA)
title_sort tween 80 improves the acid-fast bacilli quantification in the magnetic nanoparticle-based colorimetric biosensing assay (ncba)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773761/
https://www.ncbi.nlm.nih.gov/pubmed/35049656
http://dx.doi.org/10.3390/bios12010029
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