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Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men

In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry p...

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Autores principales: Berglund, Anders, Matta, Jaime, Encarnación-Medina, Jarline, Ortiz-Sanchéz, Carmen, Dutil, Julie, Linares, Raymond, Marcial, Joshua, Abreu-Takemura, Caren, Moreno, Natasha, Putney, Ryan, Chakrabarti, Ratna, Lin, Hui-Yi, Yamoah, Kosj, Osterman, Carlos Diaz, Wang, Liang, Dhillon, Jasreman, Kim, Youngchul, Kim, Seung Joon, Ruiz-Deya, Gilberto, Park, Jong Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773891/
https://www.ncbi.nlm.nih.gov/pubmed/35053153
http://dx.doi.org/10.3390/biom12010002
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author Berglund, Anders
Matta, Jaime
Encarnación-Medina, Jarline
Ortiz-Sanchéz, Carmen
Dutil, Julie
Linares, Raymond
Marcial, Joshua
Abreu-Takemura, Caren
Moreno, Natasha
Putney, Ryan
Chakrabarti, Ratna
Lin, Hui-Yi
Yamoah, Kosj
Osterman, Carlos Diaz
Wang, Liang
Dhillon, Jasreman
Kim, Youngchul
Kim, Seung Joon
Ruiz-Deya, Gilberto
Park, Jong Y.
author_facet Berglund, Anders
Matta, Jaime
Encarnación-Medina, Jarline
Ortiz-Sanchéz, Carmen
Dutil, Julie
Linares, Raymond
Marcial, Joshua
Abreu-Takemura, Caren
Moreno, Natasha
Putney, Ryan
Chakrabarti, Ratna
Lin, Hui-Yi
Yamoah, Kosj
Osterman, Carlos Diaz
Wang, Liang
Dhillon, Jasreman
Kim, Youngchul
Kim, Seung Joon
Ruiz-Deya, Gilberto
Park, Jong Y.
author_sort Berglund, Anders
collection PubMed
description In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry proportions in 49 H/L men from Puerto Rico (PR). Prostate tumors were classified as aggressive (n = 17) and indolent (n = 32) based on the Gleason score. Genomic DNA samples were extracted by macro-dissection. DNA methylation patterns were assessed using the Illumina EPIC DNA methylation platform. We used ADMIXTURE to estimate global ancestry proportions. We identified 892 differentially methylated genes in prostate tumor tissues as compared with normal tissues. Based on an epigenetic clock model, we observed that the total number of stem cell divisions (TNSC) and stem cell division rate (SCDR) were significantly higher in tumor than adjacent normal tissues. Regarding PCa aggressiveness, 141 differentially methylated genes were identified. Ancestry proportions of PR men were estimated as African, European, and Indigenous American; these were 24.1%, 64.2%, and 11.7%, respectively. The identification of DNA methylation profiles associated with risk and aggressiveness of PCa in PR H/L men will shed light on potential mechanisms contributing to PCa disparities in PR population.
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spelling pubmed-87738912022-01-21 Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men Berglund, Anders Matta, Jaime Encarnación-Medina, Jarline Ortiz-Sanchéz, Carmen Dutil, Julie Linares, Raymond Marcial, Joshua Abreu-Takemura, Caren Moreno, Natasha Putney, Ryan Chakrabarti, Ratna Lin, Hui-Yi Yamoah, Kosj Osterman, Carlos Diaz Wang, Liang Dhillon, Jasreman Kim, Youngchul Kim, Seung Joon Ruiz-Deya, Gilberto Park, Jong Y. Biomolecules Article In 2021, approximately 248,530 new prostate cancer (PCa) cases are estimated in the United States. Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. The objective of this study was to assess DNA methylation patterns between aggressive and indolent PCa along with ancestry proportions in 49 H/L men from Puerto Rico (PR). Prostate tumors were classified as aggressive (n = 17) and indolent (n = 32) based on the Gleason score. Genomic DNA samples were extracted by macro-dissection. DNA methylation patterns were assessed using the Illumina EPIC DNA methylation platform. We used ADMIXTURE to estimate global ancestry proportions. We identified 892 differentially methylated genes in prostate tumor tissues as compared with normal tissues. Based on an epigenetic clock model, we observed that the total number of stem cell divisions (TNSC) and stem cell division rate (SCDR) were significantly higher in tumor than adjacent normal tissues. Regarding PCa aggressiveness, 141 differentially methylated genes were identified. Ancestry proportions of PR men were estimated as African, European, and Indigenous American; these were 24.1%, 64.2%, and 11.7%, respectively. The identification of DNA methylation profiles associated with risk and aggressiveness of PCa in PR H/L men will shed light on potential mechanisms contributing to PCa disparities in PR population. MDPI 2021-12-21 /pmc/articles/PMC8773891/ /pubmed/35053153 http://dx.doi.org/10.3390/biom12010002 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Berglund, Anders
Matta, Jaime
Encarnación-Medina, Jarline
Ortiz-Sanchéz, Carmen
Dutil, Julie
Linares, Raymond
Marcial, Joshua
Abreu-Takemura, Caren
Moreno, Natasha
Putney, Ryan
Chakrabarti, Ratna
Lin, Hui-Yi
Yamoah, Kosj
Osterman, Carlos Diaz
Wang, Liang
Dhillon, Jasreman
Kim, Youngchul
Kim, Seung Joon
Ruiz-Deya, Gilberto
Park, Jong Y.
Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
title Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
title_full Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
title_fullStr Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
title_full_unstemmed Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
title_short Dysregulation of DNA Methylation and Epigenetic Clocks in Prostate Cancer among Puerto Rican Men
title_sort dysregulation of dna methylation and epigenetic clocks in prostate cancer among puerto rican men
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773891/
https://www.ncbi.nlm.nih.gov/pubmed/35053153
http://dx.doi.org/10.3390/biom12010002
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