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AuNP Aptasensor for Hodgkin Lymphoma Monitoring

A liquid biopsy based on circulating small extracellular vesicles (SEVs) has not yet been used in routine clinical practice due to the lack of reliable analytic technologies. Recent studies have demonstrated the great diagnostic potential of nanozyme-based systems for the detection of SEV markers. H...

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Autores principales: Slyusarenko, Maria, Shalaev, Sergey, Valitova, Alina, Zabegina, Lidia, Nikiforova, Nadezhda, Nazarova, Inga, Rudakovskaya, Polina, Vorobiev, Maxim, Lezov, Alexey, Filatova, Larisa, Yevlampieva, Natalia, Gorin, Dmitry, Krzhivitsky, Pavel, Malek, Anastasia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774100/
https://www.ncbi.nlm.nih.gov/pubmed/35049651
http://dx.doi.org/10.3390/bios12010023
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author Slyusarenko, Maria
Shalaev, Sergey
Valitova, Alina
Zabegina, Lidia
Nikiforova, Nadezhda
Nazarova, Inga
Rudakovskaya, Polina
Vorobiev, Maxim
Lezov, Alexey
Filatova, Larisa
Yevlampieva, Natalia
Gorin, Dmitry
Krzhivitsky, Pavel
Malek, Anastasia
author_facet Slyusarenko, Maria
Shalaev, Sergey
Valitova, Alina
Zabegina, Lidia
Nikiforova, Nadezhda
Nazarova, Inga
Rudakovskaya, Polina
Vorobiev, Maxim
Lezov, Alexey
Filatova, Larisa
Yevlampieva, Natalia
Gorin, Dmitry
Krzhivitsky, Pavel
Malek, Anastasia
author_sort Slyusarenko, Maria
collection PubMed
description A liquid biopsy based on circulating small extracellular vesicles (SEVs) has not yet been used in routine clinical practice due to the lack of reliable analytic technologies. Recent studies have demonstrated the great diagnostic potential of nanozyme-based systems for the detection of SEV markers. Here, we hypothesize that CD30-positive Hodgkin and Reed–Sternberg (HRS) cells secrete CD30 + SEVs; therefore, the relative amount of circulating CD30 + SEVs might reflect classical forms of Hodgkin lymphoma (cHL) activity and can be measured by using a nanozyme-based technique. A AuNP aptasensor analytics system was created using aurum nanoparticles (AuNPs) with peroxidase activity. Sensing was mediated by competing properties of DNA aptamers to attach onto surface of AuNPs inhibiting their enzymatic activity and to bind specific markers on SEVs surface. An enzymatic activity of AuNPs was evaluated through the color reaction. The study included characterization of the components of the analytic system and its functionality using transmission and scanning electron microscopy, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), and spectrophotometry. AuNP aptasensor analytics were optimized to quantify plasma CD30 + SEVs. The developed method allowed us to differentiate healthy donors and cHL patients. The results of the CD30 + SEV quantification in the plasma of cHL patients were compared with the results of disease activity assessment by positron emission tomography/computed tomography (PET-CT) scanning, revealing a strong positive correlation. Moreover, two cycles of chemotherapy resulted in a statistically significant decrease in CD30 + SEVs in the plasma of cHL patients. The proposed AuNP aptasensor system presents a promising new approach for monitoring cHL patients and can be modified for the diagnostic testing of other diseases.
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spelling pubmed-87741002022-01-21 AuNP Aptasensor for Hodgkin Lymphoma Monitoring Slyusarenko, Maria Shalaev, Sergey Valitova, Alina Zabegina, Lidia Nikiforova, Nadezhda Nazarova, Inga Rudakovskaya, Polina Vorobiev, Maxim Lezov, Alexey Filatova, Larisa Yevlampieva, Natalia Gorin, Dmitry Krzhivitsky, Pavel Malek, Anastasia Biosensors (Basel) Article A liquid biopsy based on circulating small extracellular vesicles (SEVs) has not yet been used in routine clinical practice due to the lack of reliable analytic technologies. Recent studies have demonstrated the great diagnostic potential of nanozyme-based systems for the detection of SEV markers. Here, we hypothesize that CD30-positive Hodgkin and Reed–Sternberg (HRS) cells secrete CD30 + SEVs; therefore, the relative amount of circulating CD30 + SEVs might reflect classical forms of Hodgkin lymphoma (cHL) activity and can be measured by using a nanozyme-based technique. A AuNP aptasensor analytics system was created using aurum nanoparticles (AuNPs) with peroxidase activity. Sensing was mediated by competing properties of DNA aptamers to attach onto surface of AuNPs inhibiting their enzymatic activity and to bind specific markers on SEVs surface. An enzymatic activity of AuNPs was evaluated through the color reaction. The study included characterization of the components of the analytic system and its functionality using transmission and scanning electron microscopy, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), and spectrophotometry. AuNP aptasensor analytics were optimized to quantify plasma CD30 + SEVs. The developed method allowed us to differentiate healthy donors and cHL patients. The results of the CD30 + SEV quantification in the plasma of cHL patients were compared with the results of disease activity assessment by positron emission tomography/computed tomography (PET-CT) scanning, revealing a strong positive correlation. Moreover, two cycles of chemotherapy resulted in a statistically significant decrease in CD30 + SEVs in the plasma of cHL patients. The proposed AuNP aptasensor system presents a promising new approach for monitoring cHL patients and can be modified for the diagnostic testing of other diseases. MDPI 2022-01-04 /pmc/articles/PMC8774100/ /pubmed/35049651 http://dx.doi.org/10.3390/bios12010023 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Slyusarenko, Maria
Shalaev, Sergey
Valitova, Alina
Zabegina, Lidia
Nikiforova, Nadezhda
Nazarova, Inga
Rudakovskaya, Polina
Vorobiev, Maxim
Lezov, Alexey
Filatova, Larisa
Yevlampieva, Natalia
Gorin, Dmitry
Krzhivitsky, Pavel
Malek, Anastasia
AuNP Aptasensor for Hodgkin Lymphoma Monitoring
title AuNP Aptasensor for Hodgkin Lymphoma Monitoring
title_full AuNP Aptasensor for Hodgkin Lymphoma Monitoring
title_fullStr AuNP Aptasensor for Hodgkin Lymphoma Monitoring
title_full_unstemmed AuNP Aptasensor for Hodgkin Lymphoma Monitoring
title_short AuNP Aptasensor for Hodgkin Lymphoma Monitoring
title_sort aunp aptasensor for hodgkin lymphoma monitoring
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774100/
https://www.ncbi.nlm.nih.gov/pubmed/35049651
http://dx.doi.org/10.3390/bios12010023
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