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Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model

(1) Background: Developing a high-quality, injectable biomaterial that is labor-saving, cost-efficient, and patient-ready is highly desirable. Our research group has previously developed a collagen-based injectable scaffold for the treatment of a variety of wounds including wounds with deep and irre...

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Autores principales: Alnojeidi, Hatem, Kilani, Ruhangiz Taghi, Ghahary, Aziz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774422/
https://www.ncbi.nlm.nih.gov/pubmed/35049584
http://dx.doi.org/10.3390/gels8010049
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author Alnojeidi, Hatem
Kilani, Ruhangiz Taghi
Ghahary, Aziz
author_facet Alnojeidi, Hatem
Kilani, Ruhangiz Taghi
Ghahary, Aziz
author_sort Alnojeidi, Hatem
collection PubMed
description (1) Background: Developing a high-quality, injectable biomaterial that is labor-saving, cost-efficient, and patient-ready is highly desirable. Our research group has previously developed a collagen-based injectable scaffold for the treatment of a variety of wounds including wounds with deep and irregular beds. Here, we investigated the biocompatibility of our liquid scaffold in mice and compared the results to a commercially available injectable granular collagen-based product. (2) Methods: Scaffolds were applied in sub-dermal pockets on the dorsum of mice. To examine the interaction between the scaffolds and the host tissue, samples were harvested after 1 and 2 weeks and stained for collagen content using Masson’s Trichrome staining. Immunofluorescence staining and quantification were performed to assess the type and number of cells infiltrating each scaffold. (3) Results: Histological evaluation after 1 and 2 weeks demonstrated early and efficient integration of our liquid scaffold with no evident adverse foreign body reaction. This rapid incorporation was accompanied by significant cellular infiltration of stromal and immune cells into the scaffold when compared to the commercial product (p < 0.01) and the control group (p < 0.05). Contrarily, the commercial scaffold induced a foreign body reaction as it was surrounded by a capsule-like, dense cellular layer during the 2-week period, resulting in delayed integration and hampered cellular infiltration. (4) Conclusion: Results obtained from this study demonstrate the potential use of our liquid scaffold as an advanced injectable wound matrix for the management of skin wounds with complex geometries.
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spelling pubmed-87744222022-01-21 Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model Alnojeidi, Hatem Kilani, Ruhangiz Taghi Ghahary, Aziz Gels Article (1) Background: Developing a high-quality, injectable biomaterial that is labor-saving, cost-efficient, and patient-ready is highly desirable. Our research group has previously developed a collagen-based injectable scaffold for the treatment of a variety of wounds including wounds with deep and irregular beds. Here, we investigated the biocompatibility of our liquid scaffold in mice and compared the results to a commercially available injectable granular collagen-based product. (2) Methods: Scaffolds were applied in sub-dermal pockets on the dorsum of mice. To examine the interaction between the scaffolds and the host tissue, samples were harvested after 1 and 2 weeks and stained for collagen content using Masson’s Trichrome staining. Immunofluorescence staining and quantification were performed to assess the type and number of cells infiltrating each scaffold. (3) Results: Histological evaluation after 1 and 2 weeks demonstrated early and efficient integration of our liquid scaffold with no evident adverse foreign body reaction. This rapid incorporation was accompanied by significant cellular infiltration of stromal and immune cells into the scaffold when compared to the commercial product (p < 0.01) and the control group (p < 0.05). Contrarily, the commercial scaffold induced a foreign body reaction as it was surrounded by a capsule-like, dense cellular layer during the 2-week period, resulting in delayed integration and hampered cellular infiltration. (4) Conclusion: Results obtained from this study demonstrate the potential use of our liquid scaffold as an advanced injectable wound matrix for the management of skin wounds with complex geometries. MDPI 2022-01-09 /pmc/articles/PMC8774422/ /pubmed/35049584 http://dx.doi.org/10.3390/gels8010049 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alnojeidi, Hatem
Kilani, Ruhangiz Taghi
Ghahary, Aziz
Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model
title Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model
title_full Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model
title_fullStr Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model
title_full_unstemmed Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model
title_short Evaluating the Biocompatibility of an Injectable Wound Matrix in a Murine Model
title_sort evaluating the biocompatibility of an injectable wound matrix in a murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774422/
https://www.ncbi.nlm.nih.gov/pubmed/35049584
http://dx.doi.org/10.3390/gels8010049
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