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Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region

Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of v...

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Autores principales: Cherubini, Sabrina, Perilli, Mariagrazia, Azzini, Anna Maria, Tacconelli, Evelina, Maccacaro, Laura, Bazaj, Alda, Naso, Laura, Amicosante, Gianfranco, Lo Cascio, Giuliana, Piccirilli, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774598/
https://www.ncbi.nlm.nih.gov/pubmed/35054380
http://dx.doi.org/10.3390/diagnostics12010213
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author Cherubini, Sabrina
Perilli, Mariagrazia
Azzini, Anna Maria
Tacconelli, Evelina
Maccacaro, Laura
Bazaj, Alda
Naso, Laura
Amicosante, Gianfranco
Lo Cascio, Giuliana
Piccirilli, Alessandra
author_facet Cherubini, Sabrina
Perilli, Mariagrazia
Azzini, Anna Maria
Tacconelli, Evelina
Maccacaro, Laura
Bazaj, Alda
Naso, Laura
Amicosante, Gianfranco
Lo Cascio, Giuliana
Piccirilli, Alessandra
author_sort Cherubini, Sabrina
collection PubMed
description Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of view, 43 E. coli strains collected from residents of LTCFs in Northern Italy. The most common lineage found was ST131, followed by sporadic presence of ST12, ST69, ST48, ST95, ST410 and ST1193. All strains were incubators of several virulence factors, with iss, sat, iha and senB being found in 84%, 72%, 63% and 51% of E. coli, respectively. Thirty of the ST131 analyzed were of the O25b:H4 serotype and H30 subclone. The ST131 isolates were found to be mainly associated with IncF plasmids, CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-27 and gyrA/parC/parE mutations. Metallo-β-lactamases were not found in ST131, whereas KPC-3 carbapenemase was found only in two ST131 and one ST1193. In conclusion, we confirmed the spread of extended-spectrum β-lactamase genes in E. coli ST131 isolated from colonized residents living inside LTCFs. The ST131 represents an incubator of fluoroquinolones, aminoglycosides and other antibiotic resistance genes in addition to different virulence factors.
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spelling pubmed-87745982022-01-21 Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region Cherubini, Sabrina Perilli, Mariagrazia Azzini, Anna Maria Tacconelli, Evelina Maccacaro, Laura Bazaj, Alda Naso, Laura Amicosante, Gianfranco Lo Cascio, Giuliana Piccirilli, Alessandra Diagnostics (Basel) Article Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of view, 43 E. coli strains collected from residents of LTCFs in Northern Italy. The most common lineage found was ST131, followed by sporadic presence of ST12, ST69, ST48, ST95, ST410 and ST1193. All strains were incubators of several virulence factors, with iss, sat, iha and senB being found in 84%, 72%, 63% and 51% of E. coli, respectively. Thirty of the ST131 analyzed were of the O25b:H4 serotype and H30 subclone. The ST131 isolates were found to be mainly associated with IncF plasmids, CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-27 and gyrA/parC/parE mutations. Metallo-β-lactamases were not found in ST131, whereas KPC-3 carbapenemase was found only in two ST131 and one ST1193. In conclusion, we confirmed the spread of extended-spectrum β-lactamase genes in E. coli ST131 isolated from colonized residents living inside LTCFs. The ST131 represents an incubator of fluoroquinolones, aminoglycosides and other antibiotic resistance genes in addition to different virulence factors. MDPI 2022-01-16 /pmc/articles/PMC8774598/ /pubmed/35054380 http://dx.doi.org/10.3390/diagnostics12010213 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cherubini, Sabrina
Perilli, Mariagrazia
Azzini, Anna Maria
Tacconelli, Evelina
Maccacaro, Laura
Bazaj, Alda
Naso, Laura
Amicosante, Gianfranco
Lo Cascio, Giuliana
Piccirilli, Alessandra
Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
title Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
title_full Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
title_fullStr Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
title_full_unstemmed Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
title_short Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
title_sort resistome and virulome of multi-drug resistant e. coli st131 isolated from residents of long-term care facilities in the northern italian region
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774598/
https://www.ncbi.nlm.nih.gov/pubmed/35054380
http://dx.doi.org/10.3390/diagnostics12010213
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