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Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region
Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of v...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774598/ https://www.ncbi.nlm.nih.gov/pubmed/35054380 http://dx.doi.org/10.3390/diagnostics12010213 |
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author | Cherubini, Sabrina Perilli, Mariagrazia Azzini, Anna Maria Tacconelli, Evelina Maccacaro, Laura Bazaj, Alda Naso, Laura Amicosante, Gianfranco Lo Cascio, Giuliana Piccirilli, Alessandra |
author_facet | Cherubini, Sabrina Perilli, Mariagrazia Azzini, Anna Maria Tacconelli, Evelina Maccacaro, Laura Bazaj, Alda Naso, Laura Amicosante, Gianfranco Lo Cascio, Giuliana Piccirilli, Alessandra |
author_sort | Cherubini, Sabrina |
collection | PubMed |
description | Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of view, 43 E. coli strains collected from residents of LTCFs in Northern Italy. The most common lineage found was ST131, followed by sporadic presence of ST12, ST69, ST48, ST95, ST410 and ST1193. All strains were incubators of several virulence factors, with iss, sat, iha and senB being found in 84%, 72%, 63% and 51% of E. coli, respectively. Thirty of the ST131 analyzed were of the O25b:H4 serotype and H30 subclone. The ST131 isolates were found to be mainly associated with IncF plasmids, CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-27 and gyrA/parC/parE mutations. Metallo-β-lactamases were not found in ST131, whereas KPC-3 carbapenemase was found only in two ST131 and one ST1193. In conclusion, we confirmed the spread of extended-spectrum β-lactamase genes in E. coli ST131 isolated from colonized residents living inside LTCFs. The ST131 represents an incubator of fluoroquinolones, aminoglycosides and other antibiotic resistance genes in addition to different virulence factors. |
format | Online Article Text |
id | pubmed-8774598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87745982022-01-21 Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region Cherubini, Sabrina Perilli, Mariagrazia Azzini, Anna Maria Tacconelli, Evelina Maccacaro, Laura Bazaj, Alda Naso, Laura Amicosante, Gianfranco Lo Cascio, Giuliana Piccirilli, Alessandra Diagnostics (Basel) Article Long-term care facilities (LTCFs) are important reservoirs of antimicrobial-resistant (AMR) bacteria which colonize patients transferred from the hospital, or they may emerge in the facility as a result of mutation or gene transfer. In the present study, we characterized, from a molecular point of view, 43 E. coli strains collected from residents of LTCFs in Northern Italy. The most common lineage found was ST131, followed by sporadic presence of ST12, ST69, ST48, ST95, ST410 and ST1193. All strains were incubators of several virulence factors, with iss, sat, iha and senB being found in 84%, 72%, 63% and 51% of E. coli, respectively. Thirty of the ST131 analyzed were of the O25b:H4 serotype and H30 subclone. The ST131 isolates were found to be mainly associated with IncF plasmids, CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-27 and gyrA/parC/parE mutations. Metallo-β-lactamases were not found in ST131, whereas KPC-3 carbapenemase was found only in two ST131 and one ST1193. In conclusion, we confirmed the spread of extended-spectrum β-lactamase genes in E. coli ST131 isolated from colonized residents living inside LTCFs. The ST131 represents an incubator of fluoroquinolones, aminoglycosides and other antibiotic resistance genes in addition to different virulence factors. MDPI 2022-01-16 /pmc/articles/PMC8774598/ /pubmed/35054380 http://dx.doi.org/10.3390/diagnostics12010213 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cherubini, Sabrina Perilli, Mariagrazia Azzini, Anna Maria Tacconelli, Evelina Maccacaro, Laura Bazaj, Alda Naso, Laura Amicosante, Gianfranco Lo Cascio, Giuliana Piccirilli, Alessandra Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region |
title | Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region |
title_full | Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region |
title_fullStr | Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region |
title_full_unstemmed | Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region |
title_short | Resistome and Virulome of Multi-Drug Resistant E. coli ST131 Isolated from Residents of Long-Term Care Facilities in the Northern Italian Region |
title_sort | resistome and virulome of multi-drug resistant e. coli st131 isolated from residents of long-term care facilities in the northern italian region |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774598/ https://www.ncbi.nlm.nih.gov/pubmed/35054380 http://dx.doi.org/10.3390/diagnostics12010213 |
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