Cargando…
Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
The production of patient-specific bone substitutes with an exact fit through 3D printing is emerging as an alternative to autologous bone grafting. To the success of tissue regeneration, the material characteristics such as porosity, stiffness, and surface topography have a strong influence on the...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774978/ https://www.ncbi.nlm.nih.gov/pubmed/35049563 http://dx.doi.org/10.3390/gels8010028 |
_version_ | 1784636471040278528 |
---|---|
author | Herrada-Manchón, Helena Rodríguez-González, David Fernández, Manuel Alejandro Kucko, Nathan William Barrère-de Groot, Florence Aguilar, Enrique |
author_facet | Herrada-Manchón, Helena Rodríguez-González, David Fernández, Manuel Alejandro Kucko, Nathan William Barrère-de Groot, Florence Aguilar, Enrique |
author_sort | Herrada-Manchón, Helena |
collection | PubMed |
description | The production of patient-specific bone substitutes with an exact fit through 3D printing is emerging as an alternative to autologous bone grafting. To the success of tissue regeneration, the material characteristics such as porosity, stiffness, and surface topography have a strong influence on the cell–material interaction and require significant attention. Printing a soft hydrocolloid-based hydrogel reinforced with irregularly-shaped microporous biphasic calcium phosphate (BCP) particles (150–500 µm) is an alternative strategy for the acquisition of a complex network with good mechanical properties that could fulfill the needs of cell proliferation and regeneration. Three well-known hydrocolloids (sodium alginate, xanthan gum, and gelatin) have been combined with BCP particles to generate stable, homogenous, and printable solid dispersions. Through rheological assessment, it was determined that the crosslinking time, printing process parameters (infill density percentage and infill pattern), as well as BCP particle size and concentration all influence the stiffness of the printed matrices. Additionally, the swelling behavior on fresh and dehydrated 3D-printed structures was investigated, where it was observed that the BCP particle characteristics influenced the constructs’ water absorption, particle diffusion out of the matrix and degradability. |
format | Online Article Text |
id | pubmed-8774978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87749782022-01-21 Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels Herrada-Manchón, Helena Rodríguez-González, David Fernández, Manuel Alejandro Kucko, Nathan William Barrère-de Groot, Florence Aguilar, Enrique Gels Article The production of patient-specific bone substitutes with an exact fit through 3D printing is emerging as an alternative to autologous bone grafting. To the success of tissue regeneration, the material characteristics such as porosity, stiffness, and surface topography have a strong influence on the cell–material interaction and require significant attention. Printing a soft hydrocolloid-based hydrogel reinforced with irregularly-shaped microporous biphasic calcium phosphate (BCP) particles (150–500 µm) is an alternative strategy for the acquisition of a complex network with good mechanical properties that could fulfill the needs of cell proliferation and regeneration. Three well-known hydrocolloids (sodium alginate, xanthan gum, and gelatin) have been combined with BCP particles to generate stable, homogenous, and printable solid dispersions. Through rheological assessment, it was determined that the crosslinking time, printing process parameters (infill density percentage and infill pattern), as well as BCP particle size and concentration all influence the stiffness of the printed matrices. Additionally, the swelling behavior on fresh and dehydrated 3D-printed structures was investigated, where it was observed that the BCP particle characteristics influenced the constructs’ water absorption, particle diffusion out of the matrix and degradability. MDPI 2022-01-02 /pmc/articles/PMC8774978/ /pubmed/35049563 http://dx.doi.org/10.3390/gels8010028 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Herrada-Manchón, Helena Rodríguez-González, David Fernández, Manuel Alejandro Kucko, Nathan William Barrère-de Groot, Florence Aguilar, Enrique Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels |
title | Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels |
title_full | Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels |
title_fullStr | Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels |
title_full_unstemmed | Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels |
title_short | Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels |
title_sort | effect on rheological properties and 3d printability of biphasic calcium phosphate microporous particles in hydrocolloid-based hydrogels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774978/ https://www.ncbi.nlm.nih.gov/pubmed/35049563 http://dx.doi.org/10.3390/gels8010028 |
work_keys_str_mv | AT herradamanchonhelena effectonrheologicalpropertiesand3dprintabilityofbiphasiccalciumphosphatemicroporousparticlesinhydrocolloidbasedhydrogels AT rodriguezgonzalezdavid effectonrheologicalpropertiesand3dprintabilityofbiphasiccalciumphosphatemicroporousparticlesinhydrocolloidbasedhydrogels AT fernandezmanuelalejandro effectonrheologicalpropertiesand3dprintabilityofbiphasiccalciumphosphatemicroporousparticlesinhydrocolloidbasedhydrogels AT kuckonathanwilliam effectonrheologicalpropertiesand3dprintabilityofbiphasiccalciumphosphatemicroporousparticlesinhydrocolloidbasedhydrogels AT barreredegrootflorence effectonrheologicalpropertiesand3dprintabilityofbiphasiccalciumphosphatemicroporousparticlesinhydrocolloidbasedhydrogels AT aguilarenrique effectonrheologicalpropertiesand3dprintabilityofbiphasiccalciumphosphatemicroporousparticlesinhydrocolloidbasedhydrogels |