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Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels

The production of patient-specific bone substitutes with an exact fit through 3D printing is emerging as an alternative to autologous bone grafting. To the success of tissue regeneration, the material characteristics such as porosity, stiffness, and surface topography have a strong influence on the...

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Autores principales: Herrada-Manchón, Helena, Rodríguez-González, David, Fernández, Manuel Alejandro, Kucko, Nathan William, Barrère-de Groot, Florence, Aguilar, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774978/
https://www.ncbi.nlm.nih.gov/pubmed/35049563
http://dx.doi.org/10.3390/gels8010028
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author Herrada-Manchón, Helena
Rodríguez-González, David
Fernández, Manuel Alejandro
Kucko, Nathan William
Barrère-de Groot, Florence
Aguilar, Enrique
author_facet Herrada-Manchón, Helena
Rodríguez-González, David
Fernández, Manuel Alejandro
Kucko, Nathan William
Barrère-de Groot, Florence
Aguilar, Enrique
author_sort Herrada-Manchón, Helena
collection PubMed
description The production of patient-specific bone substitutes with an exact fit through 3D printing is emerging as an alternative to autologous bone grafting. To the success of tissue regeneration, the material characteristics such as porosity, stiffness, and surface topography have a strong influence on the cell–material interaction and require significant attention. Printing a soft hydrocolloid-based hydrogel reinforced with irregularly-shaped microporous biphasic calcium phosphate (BCP) particles (150–500 µm) is an alternative strategy for the acquisition of a complex network with good mechanical properties that could fulfill the needs of cell proliferation and regeneration. Three well-known hydrocolloids (sodium alginate, xanthan gum, and gelatin) have been combined with BCP particles to generate stable, homogenous, and printable solid dispersions. Through rheological assessment, it was determined that the crosslinking time, printing process parameters (infill density percentage and infill pattern), as well as BCP particle size and concentration all influence the stiffness of the printed matrices. Additionally, the swelling behavior on fresh and dehydrated 3D-printed structures was investigated, where it was observed that the BCP particle characteristics influenced the constructs’ water absorption, particle diffusion out of the matrix and degradability.
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spelling pubmed-87749782022-01-21 Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels Herrada-Manchón, Helena Rodríguez-González, David Fernández, Manuel Alejandro Kucko, Nathan William Barrère-de Groot, Florence Aguilar, Enrique Gels Article The production of patient-specific bone substitutes with an exact fit through 3D printing is emerging as an alternative to autologous bone grafting. To the success of tissue regeneration, the material characteristics such as porosity, stiffness, and surface topography have a strong influence on the cell–material interaction and require significant attention. Printing a soft hydrocolloid-based hydrogel reinforced with irregularly-shaped microporous biphasic calcium phosphate (BCP) particles (150–500 µm) is an alternative strategy for the acquisition of a complex network with good mechanical properties that could fulfill the needs of cell proliferation and regeneration. Three well-known hydrocolloids (sodium alginate, xanthan gum, and gelatin) have been combined with BCP particles to generate stable, homogenous, and printable solid dispersions. Through rheological assessment, it was determined that the crosslinking time, printing process parameters (infill density percentage and infill pattern), as well as BCP particle size and concentration all influence the stiffness of the printed matrices. Additionally, the swelling behavior on fresh and dehydrated 3D-printed structures was investigated, where it was observed that the BCP particle characteristics influenced the constructs’ water absorption, particle diffusion out of the matrix and degradability. MDPI 2022-01-02 /pmc/articles/PMC8774978/ /pubmed/35049563 http://dx.doi.org/10.3390/gels8010028 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Herrada-Manchón, Helena
Rodríguez-González, David
Fernández, Manuel Alejandro
Kucko, Nathan William
Barrère-de Groot, Florence
Aguilar, Enrique
Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
title Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
title_full Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
title_fullStr Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
title_full_unstemmed Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
title_short Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels
title_sort effect on rheological properties and 3d printability of biphasic calcium phosphate microporous particles in hydrocolloid-based hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774978/
https://www.ncbi.nlm.nih.gov/pubmed/35049563
http://dx.doi.org/10.3390/gels8010028
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