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Genetic Rescue of the Highly Inbred Norwegian Lundehund
Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norweg...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775414/ https://www.ncbi.nlm.nih.gov/pubmed/35052503 http://dx.doi.org/10.3390/genes13010163 |
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author | Melis, Claudia Pertoldi, Cino Ludington, William Basil Beuchat, Carol Qvigstad, Gunnar Stronen, Astrid Vik |
author_facet | Melis, Claudia Pertoldi, Cino Ludington, William Basil Beuchat, Carol Qvigstad, Gunnar Stronen, Astrid Vik |
author_sort | Melis, Claudia |
collection | PubMed |
description | Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individuals are vital for managing the rescue process, and we genotyped the Lundehund (n = 12), the Buhund (n = 12), their crosses (F1, n = 7) and first-generation backcrosses to the Lundehund (F2, n = 12) with >170,000 single nucleotide polymorphism loci to compare their levels of genetic diversity. We predicted that genome-wide diversity in F2 dogs would be higher than in the Lundehund but lower than in the F1 and the Buhund, and the heterozygosity values showed the expected patterns. We also found that runs of homozygosity, extended chromosomal regions of homozygous genotypes inherited from a common ancestor, were reduced in F2 individuals compared with Lundehund individuals. Our analyses demonstrate the benefits of outcrossing but indicate that some of the acquired genetic diversity is lost following immediate backcrossing. Additional breeding among F2 crosses could therefore merit from further consideration in genetic rescue management. |
format | Online Article Text |
id | pubmed-8775414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87754142022-01-21 Genetic Rescue of the Highly Inbred Norwegian Lundehund Melis, Claudia Pertoldi, Cino Ludington, William Basil Beuchat, Carol Qvigstad, Gunnar Stronen, Astrid Vik Genes (Basel) Article Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individuals are vital for managing the rescue process, and we genotyped the Lundehund (n = 12), the Buhund (n = 12), their crosses (F1, n = 7) and first-generation backcrosses to the Lundehund (F2, n = 12) with >170,000 single nucleotide polymorphism loci to compare their levels of genetic diversity. We predicted that genome-wide diversity in F2 dogs would be higher than in the Lundehund but lower than in the F1 and the Buhund, and the heterozygosity values showed the expected patterns. We also found that runs of homozygosity, extended chromosomal regions of homozygous genotypes inherited from a common ancestor, were reduced in F2 individuals compared with Lundehund individuals. Our analyses demonstrate the benefits of outcrossing but indicate that some of the acquired genetic diversity is lost following immediate backcrossing. Additional breeding among F2 crosses could therefore merit from further consideration in genetic rescue management. MDPI 2022-01-17 /pmc/articles/PMC8775414/ /pubmed/35052503 http://dx.doi.org/10.3390/genes13010163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Melis, Claudia Pertoldi, Cino Ludington, William Basil Beuchat, Carol Qvigstad, Gunnar Stronen, Astrid Vik Genetic Rescue of the Highly Inbred Norwegian Lundehund |
title | Genetic Rescue of the Highly Inbred Norwegian Lundehund |
title_full | Genetic Rescue of the Highly Inbred Norwegian Lundehund |
title_fullStr | Genetic Rescue of the Highly Inbred Norwegian Lundehund |
title_full_unstemmed | Genetic Rescue of the Highly Inbred Norwegian Lundehund |
title_short | Genetic Rescue of the Highly Inbred Norwegian Lundehund |
title_sort | genetic rescue of the highly inbred norwegian lundehund |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775414/ https://www.ncbi.nlm.nih.gov/pubmed/35052503 http://dx.doi.org/10.3390/genes13010163 |
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