Cargando…

Genetic Rescue of the Highly Inbred Norwegian Lundehund

Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norweg...

Descripción completa

Detalles Bibliográficos
Autores principales: Melis, Claudia, Pertoldi, Cino, Ludington, William Basil, Beuchat, Carol, Qvigstad, Gunnar, Stronen, Astrid Vik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775414/
https://www.ncbi.nlm.nih.gov/pubmed/35052503
http://dx.doi.org/10.3390/genes13010163
_version_ 1784636579254370304
author Melis, Claudia
Pertoldi, Cino
Ludington, William Basil
Beuchat, Carol
Qvigstad, Gunnar
Stronen, Astrid Vik
author_facet Melis, Claudia
Pertoldi, Cino
Ludington, William Basil
Beuchat, Carol
Qvigstad, Gunnar
Stronen, Astrid Vik
author_sort Melis, Claudia
collection PubMed
description Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individuals are vital for managing the rescue process, and we genotyped the Lundehund (n = 12), the Buhund (n = 12), their crosses (F1, n = 7) and first-generation backcrosses to the Lundehund (F2, n = 12) with >170,000 single nucleotide polymorphism loci to compare their levels of genetic diversity. We predicted that genome-wide diversity in F2 dogs would be higher than in the Lundehund but lower than in the F1 and the Buhund, and the heterozygosity values showed the expected patterns. We also found that runs of homozygosity, extended chromosomal regions of homozygous genotypes inherited from a common ancestor, were reduced in F2 individuals compared with Lundehund individuals. Our analyses demonstrate the benefits of outcrossing but indicate that some of the acquired genetic diversity is lost following immediate backcrossing. Additional breeding among F2 crosses could therefore merit from further consideration in genetic rescue management.
format Online
Article
Text
id pubmed-8775414
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87754142022-01-21 Genetic Rescue of the Highly Inbred Norwegian Lundehund Melis, Claudia Pertoldi, Cino Ludington, William Basil Beuchat, Carol Qvigstad, Gunnar Stronen, Astrid Vik Genes (Basel) Article Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue”. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, including the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individuals are vital for managing the rescue process, and we genotyped the Lundehund (n = 12), the Buhund (n = 12), their crosses (F1, n = 7) and first-generation backcrosses to the Lundehund (F2, n = 12) with >170,000 single nucleotide polymorphism loci to compare their levels of genetic diversity. We predicted that genome-wide diversity in F2 dogs would be higher than in the Lundehund but lower than in the F1 and the Buhund, and the heterozygosity values showed the expected patterns. We also found that runs of homozygosity, extended chromosomal regions of homozygous genotypes inherited from a common ancestor, were reduced in F2 individuals compared with Lundehund individuals. Our analyses demonstrate the benefits of outcrossing but indicate that some of the acquired genetic diversity is lost following immediate backcrossing. Additional breeding among F2 crosses could therefore merit from further consideration in genetic rescue management. MDPI 2022-01-17 /pmc/articles/PMC8775414/ /pubmed/35052503 http://dx.doi.org/10.3390/genes13010163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Melis, Claudia
Pertoldi, Cino
Ludington, William Basil
Beuchat, Carol
Qvigstad, Gunnar
Stronen, Astrid Vik
Genetic Rescue of the Highly Inbred Norwegian Lundehund
title Genetic Rescue of the Highly Inbred Norwegian Lundehund
title_full Genetic Rescue of the Highly Inbred Norwegian Lundehund
title_fullStr Genetic Rescue of the Highly Inbred Norwegian Lundehund
title_full_unstemmed Genetic Rescue of the Highly Inbred Norwegian Lundehund
title_short Genetic Rescue of the Highly Inbred Norwegian Lundehund
title_sort genetic rescue of the highly inbred norwegian lundehund
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775414/
https://www.ncbi.nlm.nih.gov/pubmed/35052503
http://dx.doi.org/10.3390/genes13010163
work_keys_str_mv AT melisclaudia geneticrescueofthehighlyinbrednorwegianlundehund
AT pertoldicino geneticrescueofthehighlyinbrednorwegianlundehund
AT ludingtonwilliambasil geneticrescueofthehighlyinbrednorwegianlundehund
AT beuchatcarol geneticrescueofthehighlyinbrednorwegianlundehund
AT qvigstadgunnar geneticrescueofthehighlyinbrednorwegianlundehund
AT stronenastridvik geneticrescueofthehighlyinbrednorwegianlundehund