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The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment

This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyz...

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Autores principales: Flis, Damian Jozef, Bialobrodzka, Emilia Gabriela, Rodziewicz-Flis, Ewa Aleksandra, Jost, Zbigniew, Borkowska, Andzelika, Ziolkowski, Wieslaw, Kaczor, Jan Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775511/
https://www.ncbi.nlm.nih.gov/pubmed/35054933
http://dx.doi.org/10.3390/ijms23020748
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author Flis, Damian Jozef
Bialobrodzka, Emilia Gabriela
Rodziewicz-Flis, Ewa Aleksandra
Jost, Zbigniew
Borkowska, Andzelika
Ziolkowski, Wieslaw
Kaczor, Jan Jacek
author_facet Flis, Damian Jozef
Bialobrodzka, Emilia Gabriela
Rodziewicz-Flis, Ewa Aleksandra
Jost, Zbigniew
Borkowska, Andzelika
Ziolkowski, Wieslaw
Kaczor, Jan Jacek
author_sort Flis, Damian Jozef
collection PubMed
description This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyzed data, rats were randomly divided into four groups: saline treatment non-exercise and exercise and Dex treatment non-exercised and exercised. Animals in both exercised groups underwent long-lasting swimming. The concentration of lipids metabolites, glucose, and lactate were measured in skeletal muscles and blood according to standard colorimetric and fluorimetric methods. Also, activities of enzymes related to aerobic and anaerobic metabolism were measured in skeletal muscles. The results indicated that Dex treatment induced body mass loss and increased lipid metabolites in the rats’ blood but did not alter these changes in skeletal muscles. Interestingly, prolonged swimming applied after 9 days of Dex treatment significantly intensified changes induced by Dex; however, there was no difference in skeletal muscle enzymatic activities. This study shows for the first time the cumulative effect of exercise and Dex on selected elements of lipid metabolism, which seems to be essential for the patient’s health due to the common use of glucocorticoids like Dex.
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spelling pubmed-87755112022-01-21 The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment Flis, Damian Jozef Bialobrodzka, Emilia Gabriela Rodziewicz-Flis, Ewa Aleksandra Jost, Zbigniew Borkowska, Andzelika Ziolkowski, Wieslaw Kaczor, Jan Jacek Int J Mol Sci Article This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyzed data, rats were randomly divided into four groups: saline treatment non-exercise and exercise and Dex treatment non-exercised and exercised. Animals in both exercised groups underwent long-lasting swimming. The concentration of lipids metabolites, glucose, and lactate were measured in skeletal muscles and blood according to standard colorimetric and fluorimetric methods. Also, activities of enzymes related to aerobic and anaerobic metabolism were measured in skeletal muscles. The results indicated that Dex treatment induced body mass loss and increased lipid metabolites in the rats’ blood but did not alter these changes in skeletal muscles. Interestingly, prolonged swimming applied after 9 days of Dex treatment significantly intensified changes induced by Dex; however, there was no difference in skeletal muscle enzymatic activities. This study shows for the first time the cumulative effect of exercise and Dex on selected elements of lipid metabolism, which seems to be essential for the patient’s health due to the common use of glucocorticoids like Dex. MDPI 2022-01-11 /pmc/articles/PMC8775511/ /pubmed/35054933 http://dx.doi.org/10.3390/ijms23020748 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Flis, Damian Jozef
Bialobrodzka, Emilia Gabriela
Rodziewicz-Flis, Ewa Aleksandra
Jost, Zbigniew
Borkowska, Andzelika
Ziolkowski, Wieslaw
Kaczor, Jan Jacek
The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
title The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
title_full The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
title_fullStr The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
title_full_unstemmed The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
title_short The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
title_sort effect of long-lasting swimming on rats skeletal muscles energy metabolism after nine days of dexamethasone treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775511/
https://www.ncbi.nlm.nih.gov/pubmed/35054933
http://dx.doi.org/10.3390/ijms23020748
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