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The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment
This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyz...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775511/ https://www.ncbi.nlm.nih.gov/pubmed/35054933 http://dx.doi.org/10.3390/ijms23020748 |
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author | Flis, Damian Jozef Bialobrodzka, Emilia Gabriela Rodziewicz-Flis, Ewa Aleksandra Jost, Zbigniew Borkowska, Andzelika Ziolkowski, Wieslaw Kaczor, Jan Jacek |
author_facet | Flis, Damian Jozef Bialobrodzka, Emilia Gabriela Rodziewicz-Flis, Ewa Aleksandra Jost, Zbigniew Borkowska, Andzelika Ziolkowski, Wieslaw Kaczor, Jan Jacek |
author_sort | Flis, Damian Jozef |
collection | PubMed |
description | This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyzed data, rats were randomly divided into four groups: saline treatment non-exercise and exercise and Dex treatment non-exercised and exercised. Animals in both exercised groups underwent long-lasting swimming. The concentration of lipids metabolites, glucose, and lactate were measured in skeletal muscles and blood according to standard colorimetric and fluorimetric methods. Also, activities of enzymes related to aerobic and anaerobic metabolism were measured in skeletal muscles. The results indicated that Dex treatment induced body mass loss and increased lipid metabolites in the rats’ blood but did not alter these changes in skeletal muscles. Interestingly, prolonged swimming applied after 9 days of Dex treatment significantly intensified changes induced by Dex; however, there was no difference in skeletal muscle enzymatic activities. This study shows for the first time the cumulative effect of exercise and Dex on selected elements of lipid metabolism, which seems to be essential for the patient’s health due to the common use of glucocorticoids like Dex. |
format | Online Article Text |
id | pubmed-8775511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87755112022-01-21 The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment Flis, Damian Jozef Bialobrodzka, Emilia Gabriela Rodziewicz-Flis, Ewa Aleksandra Jost, Zbigniew Borkowska, Andzelika Ziolkowski, Wieslaw Kaczor, Jan Jacek Int J Mol Sci Article This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyzed data, rats were randomly divided into four groups: saline treatment non-exercise and exercise and Dex treatment non-exercised and exercised. Animals in both exercised groups underwent long-lasting swimming. The concentration of lipids metabolites, glucose, and lactate were measured in skeletal muscles and blood according to standard colorimetric and fluorimetric methods. Also, activities of enzymes related to aerobic and anaerobic metabolism were measured in skeletal muscles. The results indicated that Dex treatment induced body mass loss and increased lipid metabolites in the rats’ blood but did not alter these changes in skeletal muscles. Interestingly, prolonged swimming applied after 9 days of Dex treatment significantly intensified changes induced by Dex; however, there was no difference in skeletal muscle enzymatic activities. This study shows for the first time the cumulative effect of exercise and Dex on selected elements of lipid metabolism, which seems to be essential for the patient’s health due to the common use of glucocorticoids like Dex. MDPI 2022-01-11 /pmc/articles/PMC8775511/ /pubmed/35054933 http://dx.doi.org/10.3390/ijms23020748 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Flis, Damian Jozef Bialobrodzka, Emilia Gabriela Rodziewicz-Flis, Ewa Aleksandra Jost, Zbigniew Borkowska, Andzelika Ziolkowski, Wieslaw Kaczor, Jan Jacek The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment |
title | The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment |
title_full | The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment |
title_fullStr | The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment |
title_full_unstemmed | The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment |
title_short | The Effect of Long-Lasting Swimming on Rats Skeletal Muscles Energy Metabolism after Nine Days of Dexamethasone Treatment |
title_sort | effect of long-lasting swimming on rats skeletal muscles energy metabolism after nine days of dexamethasone treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775511/ https://www.ncbi.nlm.nih.gov/pubmed/35054933 http://dx.doi.org/10.3390/ijms23020748 |
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