RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni

The efficiency of the RNA-guided AsCas12a nuclease of Acidaminococcus sp. was compared with SpCas9 from Streptococcus pyogenes, for functional genomics in Schistosoma mansoni. We deployed optimized conditions for the ratio of guide RNAs to the nuclease, donor templates, and electroporation parameter...

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Detalles Bibliográficos
Autores principales: Ittiprasert, Wannaporn, Chatupheeraphat, Chawalit, Mann, Victoria H., Li, Wenhui, Miller, André, Ogunbayo, Taiwo, Tran, Kenny, Alrefaei, Yousef N., Mentink-Kane, Margaret, Brindley, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775552/
https://www.ncbi.nlm.nih.gov/pubmed/35054816
http://dx.doi.org/10.3390/ijms23020631

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