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Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells

Periodontitis is an inflammatory condition that causes the destruction of the supporting tissues of teeth and is a major public health problem affecting more than half of the adult population worldwide. Recently, members of the herpes virus family, such as the Epstein–Barr virus (EBV), have been sug...

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Autores principales: Yokoe, Sho, Hasuike, Akira, Watanabe, Norihisa, Tanaka, Hideki, Karahashi, Hiroyuki, Wakuda, Shin, Takeichi, Osamu, Kawato, Takayuki, Takai, Hideki, Ogata, Yorimasa, Sato, Shuichi, Imai, Kenichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775710/
https://www.ncbi.nlm.nih.gov/pubmed/35054995
http://dx.doi.org/10.3390/ijms23020809
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author Yokoe, Sho
Hasuike, Akira
Watanabe, Norihisa
Tanaka, Hideki
Karahashi, Hiroyuki
Wakuda, Shin
Takeichi, Osamu
Kawato, Takayuki
Takai, Hideki
Ogata, Yorimasa
Sato, Shuichi
Imai, Kenichi
author_facet Yokoe, Sho
Hasuike, Akira
Watanabe, Norihisa
Tanaka, Hideki
Karahashi, Hiroyuki
Wakuda, Shin
Takeichi, Osamu
Kawato, Takayuki
Takai, Hideki
Ogata, Yorimasa
Sato, Shuichi
Imai, Kenichi
author_sort Yokoe, Sho
collection PubMed
description Periodontitis is an inflammatory condition that causes the destruction of the supporting tissues of teeth and is a major public health problem affecting more than half of the adult population worldwide. Recently, members of the herpes virus family, such as the Epstein–Barr virus (EBV), have been suggested to be involved in the etiology of periodontitis because bacterial activity alone does not adequately explain the clinical characteristics of periodontitis. However, the role of EBV in the etiology of periodontitis is unknown. This study aimed to examine the effect of inactivated EBV on the expression of inflammatory cytokines in human gingival fibroblasts (HGFs) and the induction of osteoclast differentiation. We found that extremely high levels of interleukin (IL)-6 and IL-8 were induced by inactivated EBV in a copy-dependent manner in HGFs. The levels of IL-6 and IL-8 in HGFs were higher when the cells were treated with EBV than when treated with lipopolysaccharide and lipoteichoic acid. EBV induced IκBα degradation, NF-κB transcription, and RAW264.7 cell differentiation into osteoclast-like cells. These findings suggest that even without infecting the cells, EBV contributes to inflammatory cytokine production and osteoclast differentiation by contact with oral cells or macrophage lineage, resulting in periodontitis onset and progression.
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spelling pubmed-87757102022-01-21 Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells Yokoe, Sho Hasuike, Akira Watanabe, Norihisa Tanaka, Hideki Karahashi, Hiroyuki Wakuda, Shin Takeichi, Osamu Kawato, Takayuki Takai, Hideki Ogata, Yorimasa Sato, Shuichi Imai, Kenichi Int J Mol Sci Article Periodontitis is an inflammatory condition that causes the destruction of the supporting tissues of teeth and is a major public health problem affecting more than half of the adult population worldwide. Recently, members of the herpes virus family, such as the Epstein–Barr virus (EBV), have been suggested to be involved in the etiology of periodontitis because bacterial activity alone does not adequately explain the clinical characteristics of periodontitis. However, the role of EBV in the etiology of periodontitis is unknown. This study aimed to examine the effect of inactivated EBV on the expression of inflammatory cytokines in human gingival fibroblasts (HGFs) and the induction of osteoclast differentiation. We found that extremely high levels of interleukin (IL)-6 and IL-8 were induced by inactivated EBV in a copy-dependent manner in HGFs. The levels of IL-6 and IL-8 in HGFs were higher when the cells were treated with EBV than when treated with lipopolysaccharide and lipoteichoic acid. EBV induced IκBα degradation, NF-κB transcription, and RAW264.7 cell differentiation into osteoclast-like cells. These findings suggest that even without infecting the cells, EBV contributes to inflammatory cytokine production and osteoclast differentiation by contact with oral cells or macrophage lineage, resulting in periodontitis onset and progression. MDPI 2022-01-12 /pmc/articles/PMC8775710/ /pubmed/35054995 http://dx.doi.org/10.3390/ijms23020809 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yokoe, Sho
Hasuike, Akira
Watanabe, Norihisa
Tanaka, Hideki
Karahashi, Hiroyuki
Wakuda, Shin
Takeichi, Osamu
Kawato, Takayuki
Takai, Hideki
Ogata, Yorimasa
Sato, Shuichi
Imai, Kenichi
Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells
title Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells
title_full Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells
title_fullStr Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells
title_full_unstemmed Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells
title_short Epstein-Barr Virus Promotes the Production of Inflammatory Cytokines in Gingival Fibroblasts and RANKL-Induced Osteoclast Differentiation in RAW264.7 Cells
title_sort epstein-barr virus promotes the production of inflammatory cytokines in gingival fibroblasts and rankl-induced osteoclast differentiation in raw264.7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775710/
https://www.ncbi.nlm.nih.gov/pubmed/35054995
http://dx.doi.org/10.3390/ijms23020809
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