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Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer
The clonal composition of a malignant tumor strongly depends on cellular dynamics influenced by the asynchronized loss of DNA repair mechanisms. Here, our aim was to identify founder mutations leading to subsequent boosts in mutation load. The overall mutation burden in 591 colorectal cancer tumors...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775830/ https://www.ncbi.nlm.nih.gov/pubmed/35054819 http://dx.doi.org/10.3390/ijms23020633 |
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author | Pálinkás, Hajnalka Laura Pongor, Lőrinc Balajti, Máté Nagy, Ádám Nagy, Kinga Békési, Angéla Bianchini, Giampaolo Vértessy, Beáta G. Győrffy, Balázs |
author_facet | Pálinkás, Hajnalka Laura Pongor, Lőrinc Balajti, Máté Nagy, Ádám Nagy, Kinga Békési, Angéla Bianchini, Giampaolo Vértessy, Beáta G. Győrffy, Balázs |
author_sort | Pálinkás, Hajnalka Laura |
collection | PubMed |
description | The clonal composition of a malignant tumor strongly depends on cellular dynamics influenced by the asynchronized loss of DNA repair mechanisms. Here, our aim was to identify founder mutations leading to subsequent boosts in mutation load. The overall mutation burden in 591 colorectal cancer tumors was analyzed, including the mutation status of DNA-repair genes. The number of mutations was first determined across all patients and the proportion of genes having mutation in each percentile was ranked. Early mutations in DNA repair genes preceding a mutational expansion were designated as founder mutations. Survival analysis for gene expression was performed using microarray data with available relapse-free survival. Of the 180 genes involved in DNA repair, the top five founder mutations were in PRKDC (n = 31), ATM (n = 26), POLE (n = 18), SRCAP (n = 18), and BRCA2 (n = 15). PRKDC expression was 6.4-fold higher in tumors compared to normal samples, and higher expression led to longer relapse-free survival in 1211 patients (HR = 0.72, p = 4.4 × 10(−3)). In an experimental setting, the mutational load resulting from UV radiation combined with inhibition of PRKDC was analyzed. Upon treatments, the mutational load exposed a significant two-fold increase. Our results suggest PRKDC as a new key gene driving tumor heterogeneity. |
format | Online Article Text |
id | pubmed-8775830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87758302022-01-21 Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer Pálinkás, Hajnalka Laura Pongor, Lőrinc Balajti, Máté Nagy, Ádám Nagy, Kinga Békési, Angéla Bianchini, Giampaolo Vértessy, Beáta G. Győrffy, Balázs Int J Mol Sci Article The clonal composition of a malignant tumor strongly depends on cellular dynamics influenced by the asynchronized loss of DNA repair mechanisms. Here, our aim was to identify founder mutations leading to subsequent boosts in mutation load. The overall mutation burden in 591 colorectal cancer tumors was analyzed, including the mutation status of DNA-repair genes. The number of mutations was first determined across all patients and the proportion of genes having mutation in each percentile was ranked. Early mutations in DNA repair genes preceding a mutational expansion were designated as founder mutations. Survival analysis for gene expression was performed using microarray data with available relapse-free survival. Of the 180 genes involved in DNA repair, the top five founder mutations were in PRKDC (n = 31), ATM (n = 26), POLE (n = 18), SRCAP (n = 18), and BRCA2 (n = 15). PRKDC expression was 6.4-fold higher in tumors compared to normal samples, and higher expression led to longer relapse-free survival in 1211 patients (HR = 0.72, p = 4.4 × 10(−3)). In an experimental setting, the mutational load resulting from UV radiation combined with inhibition of PRKDC was analyzed. Upon treatments, the mutational load exposed a significant two-fold increase. Our results suggest PRKDC as a new key gene driving tumor heterogeneity. MDPI 2022-01-06 /pmc/articles/PMC8775830/ /pubmed/35054819 http://dx.doi.org/10.3390/ijms23020633 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pálinkás, Hajnalka Laura Pongor, Lőrinc Balajti, Máté Nagy, Ádám Nagy, Kinga Békési, Angéla Bianchini, Giampaolo Vértessy, Beáta G. Győrffy, Balázs Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer |
title | Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer |
title_full | Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer |
title_fullStr | Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer |
title_full_unstemmed | Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer |
title_short | Primary Founder Mutations in the PRKDC Gene Increase Tumor Mutation Load in Colorectal Cancer |
title_sort | primary founder mutations in the prkdc gene increase tumor mutation load in colorectal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775830/ https://www.ncbi.nlm.nih.gov/pubmed/35054819 http://dx.doi.org/10.3390/ijms23020633 |
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