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Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellula...

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Autores principales: Joushomme, Alexandre, Garenne, André, Dufossée, Mélody, Renom, Rémy, Ruigrok, Hermanus Johannes, Chappe, Yann Loick, Canovi, Anne, Patrignoni, Lorenza, Hurtier, Annabelle, Poulletier de Gannes, Florence, Lagroye, Isabelle, Lévêque, Philippe, Lewis, Noëlle, Priault, Muriel, Arnaud-Cormos, Delia, Percherancier, Yann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776001/
https://www.ncbi.nlm.nih.gov/pubmed/35054844
http://dx.doi.org/10.3390/ijms23020658
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author Joushomme, Alexandre
Garenne, André
Dufossée, Mélody
Renom, Rémy
Ruigrok, Hermanus Johannes
Chappe, Yann Loick
Canovi, Anne
Patrignoni, Lorenza
Hurtier, Annabelle
Poulletier de Gannes, Florence
Lagroye, Isabelle
Lévêque, Philippe
Lewis, Noëlle
Priault, Muriel
Arnaud-Cormos, Delia
Percherancier, Yann
author_facet Joushomme, Alexandre
Garenne, André
Dufossée, Mélody
Renom, Rémy
Ruigrok, Hermanus Johannes
Chappe, Yann Loick
Canovi, Anne
Patrignoni, Lorenza
Hurtier, Annabelle
Poulletier de Gannes, Florence
Lagroye, Isabelle
Lévêque, Philippe
Lewis, Noëlle
Priault, Muriel
Arnaud-Cormos, Delia
Percherancier, Yann
author_sort Joushomme, Alexandre
collection PubMed
description It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant.
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spelling pubmed-87760012022-01-21 Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments Joushomme, Alexandre Garenne, André Dufossée, Mélody Renom, Rémy Ruigrok, Hermanus Johannes Chappe, Yann Loick Canovi, Anne Patrignoni, Lorenza Hurtier, Annabelle Poulletier de Gannes, Florence Lagroye, Isabelle Lévêque, Philippe Lewis, Noëlle Priault, Muriel Arnaud-Cormos, Delia Percherancier, Yann Int J Mol Sci Article It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant. MDPI 2022-01-08 /pmc/articles/PMC8776001/ /pubmed/35054844 http://dx.doi.org/10.3390/ijms23020658 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Joushomme, Alexandre
Garenne, André
Dufossée, Mélody
Renom, Rémy
Ruigrok, Hermanus Johannes
Chappe, Yann Loick
Canovi, Anne
Patrignoni, Lorenza
Hurtier, Annabelle
Poulletier de Gannes, Florence
Lagroye, Isabelle
Lévêque, Philippe
Lewis, Noëlle
Priault, Muriel
Arnaud-Cormos, Delia
Percherancier, Yann
Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
title Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
title_full Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
title_fullStr Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
title_full_unstemmed Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
title_short Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
title_sort label-free study of the global cell behavior during exposure to environmental radiofrequency fields in the presence or absence of pro-apoptotic or pro-autophagic treatments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776001/
https://www.ncbi.nlm.nih.gov/pubmed/35054844
http://dx.doi.org/10.3390/ijms23020658
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