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A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing
BACKGROUND: Neurotropic virus infection can cause serious damage to the central nervous system (CNS) in both humans and animals. The complexity of the CNS poses unique challenges to investigate the infection of these viruses in the brain using traditional techniques. METHODS: In this study, we explo...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776042/ https://www.ncbi.nlm.nih.gov/pubmed/35051311 http://dx.doi.org/10.1002/ctm2.700 |
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author | Zhang, Yachun Xing, Xudong Long, Ben Cao, Yandi Hu, Simeng Li, Xiangning Yu, Yalan Tian, Dayong Sui, Baokun Luo, Zhaochen Liu, Wei Lv, Lei Wu, Qiong Dai, Jinxia Zhou, Ming Han, Heyou Fu, Zhen F. Gong, Hui Bai, Fan Zhao, Ling |
author_facet | Zhang, Yachun Xing, Xudong Long, Ben Cao, Yandi Hu, Simeng Li, Xiangning Yu, Yalan Tian, Dayong Sui, Baokun Luo, Zhaochen Liu, Wei Lv, Lei Wu, Qiong Dai, Jinxia Zhou, Ming Han, Heyou Fu, Zhen F. Gong, Hui Bai, Fan Zhao, Ling |
author_sort | Zhang, Yachun |
collection | PubMed |
description | BACKGROUND: Neurotropic virus infection can cause serious damage to the central nervous system (CNS) in both humans and animals. The complexity of the CNS poses unique challenges to investigate the infection of these viruses in the brain using traditional techniques. METHODS: In this study, we explore the use of fluorescence micro‐optical sectioning tomography (fMOST) and single‐cell RNA sequencing (scRNA‐seq) to map the spatial and cellular distribution of a representative neurotropic virus, rabies virus (RABV), in the whole brain. Mice were inoculated with a lethal dose of a recombinant RABV encoding enhanced green fluorescent protein (EGFP) under different infection routes, and a three‐dimensional (3D) view of RABV distribution in the whole mouse brain was obtained using fMOST. Meanwhile, we pinpointed the cellular distribution of RABV by utilizing scRNA‐seq. RESULTS: Our fMOST data provided the 3D view of a neurotropic virus in the whole mouse brain, which indicated that the spatial distribution of RABV in the brain was influenced by the infection route. Interestingly, we provided evidence that RABV could infect multiple nuclei related to fear independent of different infection routes. More surprisingly, our scRNA‐seq data revealed that besides neurons RABV could infect macrophages and the infiltrating macrophages played at least three different antiviral roles during RABV infection. CONCLUSION: This study draws a comprehensively spatial and cellular map of typical neurotropic virus infection in the mouse brain, providing a novel and insightful strategy to investigate the pathogenesis of RABV and other neurotropic viruses. |
format | Online Article Text |
id | pubmed-8776042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87760422022-01-24 A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing Zhang, Yachun Xing, Xudong Long, Ben Cao, Yandi Hu, Simeng Li, Xiangning Yu, Yalan Tian, Dayong Sui, Baokun Luo, Zhaochen Liu, Wei Lv, Lei Wu, Qiong Dai, Jinxia Zhou, Ming Han, Heyou Fu, Zhen F. Gong, Hui Bai, Fan Zhao, Ling Clin Transl Med Research Articles BACKGROUND: Neurotropic virus infection can cause serious damage to the central nervous system (CNS) in both humans and animals. The complexity of the CNS poses unique challenges to investigate the infection of these viruses in the brain using traditional techniques. METHODS: In this study, we explore the use of fluorescence micro‐optical sectioning tomography (fMOST) and single‐cell RNA sequencing (scRNA‐seq) to map the spatial and cellular distribution of a representative neurotropic virus, rabies virus (RABV), in the whole brain. Mice were inoculated with a lethal dose of a recombinant RABV encoding enhanced green fluorescent protein (EGFP) under different infection routes, and a three‐dimensional (3D) view of RABV distribution in the whole mouse brain was obtained using fMOST. Meanwhile, we pinpointed the cellular distribution of RABV by utilizing scRNA‐seq. RESULTS: Our fMOST data provided the 3D view of a neurotropic virus in the whole mouse brain, which indicated that the spatial distribution of RABV in the brain was influenced by the infection route. Interestingly, we provided evidence that RABV could infect multiple nuclei related to fear independent of different infection routes. More surprisingly, our scRNA‐seq data revealed that besides neurons RABV could infect macrophages and the infiltrating macrophages played at least three different antiviral roles during RABV infection. CONCLUSION: This study draws a comprehensively spatial and cellular map of typical neurotropic virus infection in the mouse brain, providing a novel and insightful strategy to investigate the pathogenesis of RABV and other neurotropic viruses. John Wiley and Sons Inc. 2022-01-20 /pmc/articles/PMC8776042/ /pubmed/35051311 http://dx.doi.org/10.1002/ctm2.700 Text en © 2022 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Zhang, Yachun Xing, Xudong Long, Ben Cao, Yandi Hu, Simeng Li, Xiangning Yu, Yalan Tian, Dayong Sui, Baokun Luo, Zhaochen Liu, Wei Lv, Lei Wu, Qiong Dai, Jinxia Zhou, Ming Han, Heyou Fu, Zhen F. Gong, Hui Bai, Fan Zhao, Ling A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing |
title | A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing |
title_full | A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing |
title_fullStr | A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing |
title_full_unstemmed | A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing |
title_short | A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing |
title_sort | spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fmost and single‐cell rna sequencing |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776042/ https://www.ncbi.nlm.nih.gov/pubmed/35051311 http://dx.doi.org/10.1002/ctm2.700 |
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