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Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan
The genus Platycerus (Coleoptera: Lucanidae) is a small stag beetle group, which is adapted to cool-temperate deciduous broad-leaved forests in East Asia. Ten Platycerus species in Japan form a monophyletic clade endemic to Japan and inhabit species-specific climatic zones. They are reported to have...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776712/ https://www.ncbi.nlm.nih.gov/pubmed/35069489 http://dx.doi.org/10.3389/fmicb.2021.793592 |
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author | Zhu, Xue-Jiao Zhang, Sheng-Nan Watanabe, Kana Kawakami, Kako Kubota, Noriko Takagi, Etsuro Tanahashi, Masahiko Wen, Xiu-Jun Kubota, Kôhei |
author_facet | Zhu, Xue-Jiao Zhang, Sheng-Nan Watanabe, Kana Kawakami, Kako Kubota, Noriko Takagi, Etsuro Tanahashi, Masahiko Wen, Xiu-Jun Kubota, Kôhei |
author_sort | Zhu, Xue-Jiao |
collection | PubMed |
description | The genus Platycerus (Coleoptera: Lucanidae) is a small stag beetle group, which is adapted to cool-temperate deciduous broad-leaved forests in East Asia. Ten Platycerus species in Japan form a monophyletic clade endemic to Japan and inhabit species-specific climatic zones. They are reported to have co-evolutionary associations with their yeast symbionts of the genus Sheffersomyces based on host cytochrome oxidase subunit I (COI) and yeast intergenic spacer (IGS) phylogenies. Here we examined the heat tolerances of the yeast colonies isolated from the mycangia of 37 females belonging ten Japanese Platycerus species. The upper limits of growth and survival temperatures of each colony were decided by cultivating it at ten temperature levels between 17.5 and 40°C. Although both temperatures varied during 25.0–31.25°C, the maximum survival temperatures (MSTs) were a little higher than the maximum growth temperatures (MGTs) in 16 colonies. Pearson’s correlations between these temperatures and environmental factors (elevation and 19 bioclimatic variables from Worldclim database) of host beetle collection sites were calculated. These temperatures were significantly correlated with elevation negatively, the maximum temperature of the warmest month (Bio5) positively, and some precipitative variables, especially in the warm season (Bio12, 13, 16, 18) negatively. Sympatric Platycerus kawadai and Platycerus albisomni share the same lineage of yeast symbionts that exhibit the same heat tolerance, but the elevational lower range limit of P. kawadai is higher than that of P. albisomni. Based on the field survey in their sympatric site, the maximum temperature of host wood of P. kawadai larvae is higher about 2–3°C than that of P. albisomni larvae in the summer, which may restrict the elevational range of P. kawadai to higher area. In conclusion, it is suggested that the heat tolerance of yeast symbionts restricts the habitat range of their host Platycerus species or/and that the environmental condition that host Platycerus species prefers affect the heat tolerance of its yeast symbionts. |
format | Online Article Text |
id | pubmed-8776712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87767122022-01-22 Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan Zhu, Xue-Jiao Zhang, Sheng-Nan Watanabe, Kana Kawakami, Kako Kubota, Noriko Takagi, Etsuro Tanahashi, Masahiko Wen, Xiu-Jun Kubota, Kôhei Front Microbiol Microbiology The genus Platycerus (Coleoptera: Lucanidae) is a small stag beetle group, which is adapted to cool-temperate deciduous broad-leaved forests in East Asia. Ten Platycerus species in Japan form a monophyletic clade endemic to Japan and inhabit species-specific climatic zones. They are reported to have co-evolutionary associations with their yeast symbionts of the genus Sheffersomyces based on host cytochrome oxidase subunit I (COI) and yeast intergenic spacer (IGS) phylogenies. Here we examined the heat tolerances of the yeast colonies isolated from the mycangia of 37 females belonging ten Japanese Platycerus species. The upper limits of growth and survival temperatures of each colony were decided by cultivating it at ten temperature levels between 17.5 and 40°C. Although both temperatures varied during 25.0–31.25°C, the maximum survival temperatures (MSTs) were a little higher than the maximum growth temperatures (MGTs) in 16 colonies. Pearson’s correlations between these temperatures and environmental factors (elevation and 19 bioclimatic variables from Worldclim database) of host beetle collection sites were calculated. These temperatures were significantly correlated with elevation negatively, the maximum temperature of the warmest month (Bio5) positively, and some precipitative variables, especially in the warm season (Bio12, 13, 16, 18) negatively. Sympatric Platycerus kawadai and Platycerus albisomni share the same lineage of yeast symbionts that exhibit the same heat tolerance, but the elevational lower range limit of P. kawadai is higher than that of P. albisomni. Based on the field survey in their sympatric site, the maximum temperature of host wood of P. kawadai larvae is higher about 2–3°C than that of P. albisomni larvae in the summer, which may restrict the elevational range of P. kawadai to higher area. In conclusion, it is suggested that the heat tolerance of yeast symbionts restricts the habitat range of their host Platycerus species or/and that the environmental condition that host Platycerus species prefers affect the heat tolerance of its yeast symbionts. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8776712/ /pubmed/35069489 http://dx.doi.org/10.3389/fmicb.2021.793592 Text en Copyright © 2022 Zhu, Zhang, Watanabe, Kawakami, Kubota, Takagi, Tanahashi, Wen and Kubota. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhu, Xue-Jiao Zhang, Sheng-Nan Watanabe, Kana Kawakami, Kako Kubota, Noriko Takagi, Etsuro Tanahashi, Masahiko Wen, Xiu-Jun Kubota, Kôhei Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan |
title | Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan |
title_full | Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan |
title_fullStr | Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan |
title_full_unstemmed | Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan |
title_short | Diverse Heat Tolerance of the Yeast Symbionts of Platycerus Stag Beetles in Japan |
title_sort | diverse heat tolerance of the yeast symbionts of platycerus stag beetles in japan |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776712/ https://www.ncbi.nlm.nih.gov/pubmed/35069489 http://dx.doi.org/10.3389/fmicb.2021.793592 |
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