Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance

The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significa...

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Detalles Bibliográficos
Autores principales: Yano, Taka-aki, Kajisa, Taira, Ono, Masayuki, Miyasaka, Yoshiya, Hasegawa, Yuichi, Saito, Atsushi, Otsuka, Kunihiro, Sakane, Ayuko, Sasaki, Takuya, Yasutomo, Koji, Hamajima, Rina, Kanai, Yuta, Kobayashi, Takeshi, Matsuura, Yoshiharu, Itonaga, Makoto, Yasui, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776812/
https://www.ncbi.nlm.nih.gov/pubmed/35058513
http://dx.doi.org/10.1038/s41598-022-05036-x
Descripción
Sumario:The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.

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