Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance

The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significa...

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Autores principales: Yano, Taka-aki, Kajisa, Taira, Ono, Masayuki, Miyasaka, Yoshiya, Hasegawa, Yuichi, Saito, Atsushi, Otsuka, Kunihiro, Sakane, Ayuko, Sasaki, Takuya, Yasutomo, Koji, Hamajima, Rina, Kanai, Yuta, Kobayashi, Takeshi, Matsuura, Yoshiharu, Itonaga, Makoto, Yasui, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776812/
https://www.ncbi.nlm.nih.gov/pubmed/35058513
http://dx.doi.org/10.1038/s41598-022-05036-x
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author Yano, Taka-aki
Kajisa, Taira
Ono, Masayuki
Miyasaka, Yoshiya
Hasegawa, Yuichi
Saito, Atsushi
Otsuka, Kunihiro
Sakane, Ayuko
Sasaki, Takuya
Yasutomo, Koji
Hamajima, Rina
Kanai, Yuta
Kobayashi, Takeshi
Matsuura, Yoshiharu
Itonaga, Makoto
Yasui, Takeshi
author_facet Yano, Taka-aki
Kajisa, Taira
Ono, Masayuki
Miyasaka, Yoshiya
Hasegawa, Yuichi
Saito, Atsushi
Otsuka, Kunihiro
Sakane, Ayuko
Sasaki, Takuya
Yasutomo, Koji
Hamajima, Rina
Kanai, Yuta
Kobayashi, Takeshi
Matsuura, Yoshiharu
Itonaga, Makoto
Yasui, Takeshi
author_sort Yano, Taka-aki
collection PubMed
description The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.
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spelling pubmed-87768122022-01-24 Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance Yano, Taka-aki Kajisa, Taira Ono, Masayuki Miyasaka, Yoshiya Hasegawa, Yuichi Saito, Atsushi Otsuka, Kunihiro Sakane, Ayuko Sasaki, Takuya Yasutomo, Koji Hamajima, Rina Kanai, Yuta Kobayashi, Takeshi Matsuura, Yoshiharu Itonaga, Makoto Yasui, Takeshi Sci Rep Article The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein. Nature Publishing Group UK 2022-01-20 /pmc/articles/PMC8776812/ /pubmed/35058513 http://dx.doi.org/10.1038/s41598-022-05036-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yano, Taka-aki
Kajisa, Taira
Ono, Masayuki
Miyasaka, Yoshiya
Hasegawa, Yuichi
Saito, Atsushi
Otsuka, Kunihiro
Sakane, Ayuko
Sasaki, Takuya
Yasutomo, Koji
Hamajima, Rina
Kanai, Yuta
Kobayashi, Takeshi
Matsuura, Yoshiharu
Itonaga, Makoto
Yasui, Takeshi
Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
title Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
title_full Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
title_fullStr Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
title_full_unstemmed Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
title_short Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
title_sort ultrasensitive detection of sars-cov-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776812/
https://www.ncbi.nlm.nih.gov/pubmed/35058513
http://dx.doi.org/10.1038/s41598-022-05036-x
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