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Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness
When considering the interactions between bacteriophages and their host, the issue of phage-resistance emergence is a key element in understanding the ecological impact of phages on the bacterial population. It is also an essential parameter for the implementation of phage therapy to combat antibiot...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776877/ https://www.ncbi.nlm.nih.gov/pubmed/34465897 http://dx.doi.org/10.1038/s41396-021-01096-5 |
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author | Markwitz, Pawel Lood, Cédric Olszak, Tomasz van Noort, Vera Lavigne, Rob Drulis-Kawa, Zuzanna |
author_facet | Markwitz, Pawel Lood, Cédric Olszak, Tomasz van Noort, Vera Lavigne, Rob Drulis-Kawa, Zuzanna |
author_sort | Markwitz, Pawel |
collection | PubMed |
description | When considering the interactions between bacteriophages and their host, the issue of phage-resistance emergence is a key element in understanding the ecological impact of phages on the bacterial population. It is also an essential parameter for the implementation of phage therapy to combat antibiotic-resistant pathogens. This study investigates the phenotypic and genetic responses of five Pseudomonas aeruginosa strains (PAO1, A5803, AA43, CHA, and PAK) to the infection by seven phages with distinct evolutionary backgrounds and recognised receptors (LPS/T4P). Emerging phage-insensitivity was generally accompanied by self and cross-resistance mechanisms. Significant differences were observed between the reference PAO1 responses compared to other clinical representatives. LPS-dependent phage infections in clinical strains selected for mutations in the “global regulatory” and “other” genes, rather than in the LPS-synthesis clusters detected in PAO1 clones. Reduced fitness, as proxied by the growth rate, was correlated with large deletion (20–500 kbp) and phage carrier state. Multi-phage resistance was significantly correlated with a reduced growth rate but only in the PAO1 population. In addition, we observed that the presence of prophages decreased the lytic phage maintenance seemingly protecting the host against carrier state and occasional lytic phage propagation, thus preventing a significant reduction in bacterial growth rate. |
format | Online Article Text |
id | pubmed-8776877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-87768772022-02-04 Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness Markwitz, Pawel Lood, Cédric Olszak, Tomasz van Noort, Vera Lavigne, Rob Drulis-Kawa, Zuzanna ISME J Article When considering the interactions between bacteriophages and their host, the issue of phage-resistance emergence is a key element in understanding the ecological impact of phages on the bacterial population. It is also an essential parameter for the implementation of phage therapy to combat antibiotic-resistant pathogens. This study investigates the phenotypic and genetic responses of five Pseudomonas aeruginosa strains (PAO1, A5803, AA43, CHA, and PAK) to the infection by seven phages with distinct evolutionary backgrounds and recognised receptors (LPS/T4P). Emerging phage-insensitivity was generally accompanied by self and cross-resistance mechanisms. Significant differences were observed between the reference PAO1 responses compared to other clinical representatives. LPS-dependent phage infections in clinical strains selected for mutations in the “global regulatory” and “other” genes, rather than in the LPS-synthesis clusters detected in PAO1 clones. Reduced fitness, as proxied by the growth rate, was correlated with large deletion (20–500 kbp) and phage carrier state. Multi-phage resistance was significantly correlated with a reduced growth rate but only in the PAO1 population. In addition, we observed that the presence of prophages decreased the lytic phage maintenance seemingly protecting the host against carrier state and occasional lytic phage propagation, thus preventing a significant reduction in bacterial growth rate. Nature Publishing Group UK 2021-08-31 2022-02 /pmc/articles/PMC8776877/ /pubmed/34465897 http://dx.doi.org/10.1038/s41396-021-01096-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Markwitz, Pawel Lood, Cédric Olszak, Tomasz van Noort, Vera Lavigne, Rob Drulis-Kawa, Zuzanna Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
title | Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
title_full | Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
title_fullStr | Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
title_full_unstemmed | Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
title_short | Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
title_sort | genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776877/ https://www.ncbi.nlm.nih.gov/pubmed/34465897 http://dx.doi.org/10.1038/s41396-021-01096-5 |
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