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Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice

Acute ischemic stroke triggers complex systemic pathological responses for which the exploration of drug resources remains a challenge. Wasp venom extracted from Vespa magnifica (Smith, 1852) is most commonly used to treat rheumatoid arthritis as well as neurological disorders. Vespakinin-M (VK), a...

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Autores principales: Zhao, Hairong, Wang, Mei, Gao, Yuan, Wu, Xiumei, Xiao, Huai, Yang, Dasong, He, Furong, Lv, Jiaming, Xie, De, Wang, Qiang, Liu, Weidong, Luo, Jingang, Yang, Zizhong, Zhang, Chenggui, Cheng, Jidong, Zhao, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776894/
https://www.ncbi.nlm.nih.gov/pubmed/35058552
http://dx.doi.org/10.1038/s42003-022-03024-5
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author Zhao, Hairong
Wang, Mei
Gao, Yuan
Wu, Xiumei
Xiao, Huai
Yang, Dasong
He, Furong
Lv, Jiaming
Xie, De
Wang, Qiang
Liu, Weidong
Luo, Jingang
Yang, Zizhong
Zhang, Chenggui
Cheng, Jidong
Zhao, Yu
author_facet Zhao, Hairong
Wang, Mei
Gao, Yuan
Wu, Xiumei
Xiao, Huai
Yang, Dasong
He, Furong
Lv, Jiaming
Xie, De
Wang, Qiang
Liu, Weidong
Luo, Jingang
Yang, Zizhong
Zhang, Chenggui
Cheng, Jidong
Zhao, Yu
author_sort Zhao, Hairong
collection PubMed
description Acute ischemic stroke triggers complex systemic pathological responses for which the exploration of drug resources remains a challenge. Wasp venom extracted from Vespa magnifica (Smith, 1852) is most commonly used to treat rheumatoid arthritis as well as neurological disorders. Vespakinin-M (VK), a natural peptide from wasp venom, has remained largely unexplored for stroke. Herein, we first confirmed the structure, stability, toxicity and distribution of VK as well as its penetration into the blood–brain barrier. VK (150 and 300 µg/kg, i.p.) was administered to improve stroke constructed by middle cerebral artery occlusion in mice. Our results indicate that VK promote functional recovery in mice after ischemia stroke, including an improvement of neurological impairment, reduction of infarct volume, maintenance of blood-brain barrier integrity, and an obstruction of the inflammatory response and oxidative stress. In addition, VK treatment led to reduced neuroinflammation and apoptosis associated with the activation of PI3K–AKT and inhibition of IκBα–NF-κB signaling pathways. Simultaneously, we confirmed that VK can combine with bradykinin receptor 2 (B2R) as detected by molecular docking, the B2R antagonist HOE140 could counteract the neuro-protective effects of VK on stroke in mice. Overall, targeting the VK–B2R interaction can be considered as a practical strategy for stroke therapy.
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spelling pubmed-87768942022-02-04 Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice Zhao, Hairong Wang, Mei Gao, Yuan Wu, Xiumei Xiao, Huai Yang, Dasong He, Furong Lv, Jiaming Xie, De Wang, Qiang Liu, Weidong Luo, Jingang Yang, Zizhong Zhang, Chenggui Cheng, Jidong Zhao, Yu Commun Biol Article Acute ischemic stroke triggers complex systemic pathological responses for which the exploration of drug resources remains a challenge. Wasp venom extracted from Vespa magnifica (Smith, 1852) is most commonly used to treat rheumatoid arthritis as well as neurological disorders. Vespakinin-M (VK), a natural peptide from wasp venom, has remained largely unexplored for stroke. Herein, we first confirmed the structure, stability, toxicity and distribution of VK as well as its penetration into the blood–brain barrier. VK (150 and 300 µg/kg, i.p.) was administered to improve stroke constructed by middle cerebral artery occlusion in mice. Our results indicate that VK promote functional recovery in mice after ischemia stroke, including an improvement of neurological impairment, reduction of infarct volume, maintenance of blood-brain barrier integrity, and an obstruction of the inflammatory response and oxidative stress. In addition, VK treatment led to reduced neuroinflammation and apoptosis associated with the activation of PI3K–AKT and inhibition of IκBα–NF-κB signaling pathways. Simultaneously, we confirmed that VK can combine with bradykinin receptor 2 (B2R) as detected by molecular docking, the B2R antagonist HOE140 could counteract the neuro-protective effects of VK on stroke in mice. Overall, targeting the VK–B2R interaction can be considered as a practical strategy for stroke therapy. Nature Publishing Group UK 2022-01-20 /pmc/articles/PMC8776894/ /pubmed/35058552 http://dx.doi.org/10.1038/s42003-022-03024-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhao, Hairong
Wang, Mei
Gao, Yuan
Wu, Xiumei
Xiao, Huai
Yang, Dasong
He, Furong
Lv, Jiaming
Xie, De
Wang, Qiang
Liu, Weidong
Luo, Jingang
Yang, Zizhong
Zhang, Chenggui
Cheng, Jidong
Zhao, Yu
Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice
title Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice
title_full Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice
title_fullStr Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice
title_full_unstemmed Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice
title_short Vespakinin-M, a natural peptide from Vespa magnifica, promotes functional recovery in stroke mice
title_sort vespakinin-m, a natural peptide from vespa magnifica, promotes functional recovery in stroke mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776894/
https://www.ncbi.nlm.nih.gov/pubmed/35058552
http://dx.doi.org/10.1038/s42003-022-03024-5
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