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CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway
Non-small cell lung cancer remains the leading cause of cancer-related deaths worldwide with high morbidity and mortality. There is an urgent need to reveal new molecular mechanisms that contribute to NSCLC progression to facilitate drug development and to improve overall survival. Much attention ha...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776987/ https://www.ncbi.nlm.nih.gov/pubmed/35069696 http://dx.doi.org/10.3389/fgene.2021.798587 |
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author | Zhu, Bo Ke, Lixia Li, Peixian Wang, Xin Yang, Lan Bai, Minghua Chen, Mailin |
author_facet | Zhu, Bo Ke, Lixia Li, Peixian Wang, Xin Yang, Lan Bai, Minghua Chen, Mailin |
author_sort | Zhu, Bo |
collection | PubMed |
description | Non-small cell lung cancer remains the leading cause of cancer-related deaths worldwide with high morbidity and mortality. There is an urgent need to reveal new molecular mechanisms that contribute to NSCLC progression to facilitate drug development and to improve overall survival. Much attention has been paid to the role of circRNAs in NSCLC development. However, the knowledge of circRNAs in NSCLC is still limited, and need to be further explored. The dysregulation of circACC1 was evaluated by qRT-PCR in NSCLC samples and cell lines. The oncogenic role of circACC1 in NSCLC progression was analyzed by CCK8 and colony formation assays. The interaction between the circACC1 and miR-29c-3p, as well as MCL-1, was verified by qRT-PCR, Western blot, luciferase reporter assay, and RIP experiment. Elevated levels of circACC1 were found in NSCLC patients and were negatively correlated with OS. Ectopic expression of circACC1 promoted the capacity of cell growth and clonogenicity, while the inhibition of circACC1 decreased the proliferation and clonogenicity potential. Mechanism studies elucidated that circACC1 contributes to cell growth via directly binding to miR-29c-3p. Transfection of miR-29c-3p mimic blocked circACC1 mediated NSCLC cell proliferation. MCL-1 is a downstream target of miR-29c-3p in NSCLC cells. The circACC1/miR-29c-3p/MCL-1 axis is important in NSCLS proliferation. |
format | Online Article Text |
id | pubmed-8776987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87769872022-01-22 CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway Zhu, Bo Ke, Lixia Li, Peixian Wang, Xin Yang, Lan Bai, Minghua Chen, Mailin Front Genet Genetics Non-small cell lung cancer remains the leading cause of cancer-related deaths worldwide with high morbidity and mortality. There is an urgent need to reveal new molecular mechanisms that contribute to NSCLC progression to facilitate drug development and to improve overall survival. Much attention has been paid to the role of circRNAs in NSCLC development. However, the knowledge of circRNAs in NSCLC is still limited, and need to be further explored. The dysregulation of circACC1 was evaluated by qRT-PCR in NSCLC samples and cell lines. The oncogenic role of circACC1 in NSCLC progression was analyzed by CCK8 and colony formation assays. The interaction between the circACC1 and miR-29c-3p, as well as MCL-1, was verified by qRT-PCR, Western blot, luciferase reporter assay, and RIP experiment. Elevated levels of circACC1 were found in NSCLC patients and were negatively correlated with OS. Ectopic expression of circACC1 promoted the capacity of cell growth and clonogenicity, while the inhibition of circACC1 decreased the proliferation and clonogenicity potential. Mechanism studies elucidated that circACC1 contributes to cell growth via directly binding to miR-29c-3p. Transfection of miR-29c-3p mimic blocked circACC1 mediated NSCLC cell proliferation. MCL-1 is a downstream target of miR-29c-3p in NSCLC cells. The circACC1/miR-29c-3p/MCL-1 axis is important in NSCLS proliferation. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8776987/ /pubmed/35069696 http://dx.doi.org/10.3389/fgene.2021.798587 Text en Copyright © 2022 Zhu, Ke, Li, Wang, Yang, Bai and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zhu, Bo Ke, Lixia Li, Peixian Wang, Xin Yang, Lan Bai, Minghua Chen, Mailin CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway |
title | CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway |
title_full | CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway |
title_fullStr | CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway |
title_full_unstemmed | CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway |
title_short | CircACC1 Promotes NSCLC Proliferation via miR-29c-3p/MCL-1 Signaling Pathway |
title_sort | circacc1 promotes nsclc proliferation via mir-29c-3p/mcl-1 signaling pathway |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776987/ https://www.ncbi.nlm.nih.gov/pubmed/35069696 http://dx.doi.org/10.3389/fgene.2021.798587 |
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