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Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean

Soybean pubescence plays an important role in insect resistance, drought tolerance, and other stresses. Hence, a deep understanding of the molecular mechanism underlying pubescence is a prerequisite to a deeper understanding of insect resistance and drought tolerance. In the present study, quantitat...

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Autores principales: Li, Yawei, Chu, Li, Liu, Xiaofeng, Zhang, Nannan, Xu, Yufei, Karikari, Benjamin, Wang, Yu, Chang, Fangguo, Liu, Zexinan, Tan, Lianmei, Yue, Han, Xing, Guangnan, Zhao, Tuanjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776989/
https://www.ncbi.nlm.nih.gov/pubmed/35069626
http://dx.doi.org/10.3389/fpls.2021.771850
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author Li, Yawei
Chu, Li
Liu, Xiaofeng
Zhang, Nannan
Xu, Yufei
Karikari, Benjamin
Wang, Yu
Chang, Fangguo
Liu, Zexinan
Tan, Lianmei
Yue, Han
Xing, Guangnan
Zhao, Tuanjie
author_facet Li, Yawei
Chu, Li
Liu, Xiaofeng
Zhang, Nannan
Xu, Yufei
Karikari, Benjamin
Wang, Yu
Chang, Fangguo
Liu, Zexinan
Tan, Lianmei
Yue, Han
Xing, Guangnan
Zhao, Tuanjie
author_sort Li, Yawei
collection PubMed
description Soybean pubescence plays an important role in insect resistance, drought tolerance, and other stresses. Hence, a deep understanding of the molecular mechanism underlying pubescence is a prerequisite to a deeper understanding of insect resistance and drought tolerance. In the present study, quantitative trait loci (QTL) mapping of pubescence traits was performed using a high-density inter-specific linkage map of one recombinant inbred line (RIL) population, designated NJRINP. It was observed that pubescence length (PL) was negatively correlated with pubescence density (PD). A total of 10 and 9 QTLs distributed on six and five chromosomes were identified with phenotypic variance (PV) of 3.0–9.9% and 0.8–15.8% for PL and PD, respectively, out of which, eight and five were novel. Most decreased PL (8 of 10) and increased PD (8 of 9) alleles were from the wild soybean PI 342618B. Based on gene annotation, Protein ANalysis THrough Evolutionary Relationships and literature search, 21 and 12 candidate genes were identified related to PL and PD, respectively. In addition, Glyma.12G187200 from major QTLs qPL-12-1 and qPD-12-2, was identified as Ps (sparse pubescence) before, having an expression level of fivefold greater in NN 86-4 than in PI 342618B, hence it might be the candidate gene that is conferring both PL and PD. Based on gene expression and cluster analysis, three and four genes were considered as the important candidate genes of PL and PD, respectively. Besides, leaves with short and dense (SD) pubescence, which are similar to the wild soybean pubescence morphology, had the highest resistance to common cutworm (CCW) in soybean. In conclusion, the findings in the present study provide a better understanding of genetic basis and candidate genes information of PL and PD and the relationship with resistance to CCW in soybean.
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spelling pubmed-87769892022-01-22 Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean Li, Yawei Chu, Li Liu, Xiaofeng Zhang, Nannan Xu, Yufei Karikari, Benjamin Wang, Yu Chang, Fangguo Liu, Zexinan Tan, Lianmei Yue, Han Xing, Guangnan Zhao, Tuanjie Front Plant Sci Plant Science Soybean pubescence plays an important role in insect resistance, drought tolerance, and other stresses. Hence, a deep understanding of the molecular mechanism underlying pubescence is a prerequisite to a deeper understanding of insect resistance and drought tolerance. In the present study, quantitative trait loci (QTL) mapping of pubescence traits was performed using a high-density inter-specific linkage map of one recombinant inbred line (RIL) population, designated NJRINP. It was observed that pubescence length (PL) was negatively correlated with pubescence density (PD). A total of 10 and 9 QTLs distributed on six and five chromosomes were identified with phenotypic variance (PV) of 3.0–9.9% and 0.8–15.8% for PL and PD, respectively, out of which, eight and five were novel. Most decreased PL (8 of 10) and increased PD (8 of 9) alleles were from the wild soybean PI 342618B. Based on gene annotation, Protein ANalysis THrough Evolutionary Relationships and literature search, 21 and 12 candidate genes were identified related to PL and PD, respectively. In addition, Glyma.12G187200 from major QTLs qPL-12-1 and qPD-12-2, was identified as Ps (sparse pubescence) before, having an expression level of fivefold greater in NN 86-4 than in PI 342618B, hence it might be the candidate gene that is conferring both PL and PD. Based on gene expression and cluster analysis, three and four genes were considered as the important candidate genes of PL and PD, respectively. Besides, leaves with short and dense (SD) pubescence, which are similar to the wild soybean pubescence morphology, had the highest resistance to common cutworm (CCW) in soybean. In conclusion, the findings in the present study provide a better understanding of genetic basis and candidate genes information of PL and PD and the relationship with resistance to CCW in soybean. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8776989/ /pubmed/35069626 http://dx.doi.org/10.3389/fpls.2021.771850 Text en Copyright © 2022 Li, Chu, Liu, Zhang, Xu, Karikari, Wang, Chang, Liu, Tan, Yue, Xing and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Yawei
Chu, Li
Liu, Xiaofeng
Zhang, Nannan
Xu, Yufei
Karikari, Benjamin
Wang, Yu
Chang, Fangguo
Liu, Zexinan
Tan, Lianmei
Yue, Han
Xing, Guangnan
Zhao, Tuanjie
Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean
title Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean
title_full Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean
title_fullStr Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean
title_full_unstemmed Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean
title_short Genetic Architecture and Candidate Genes for Pubescence Length and Density and Its Relationship With Resistance to Common Cutworm in Soybean
title_sort genetic architecture and candidate genes for pubescence length and density and its relationship with resistance to common cutworm in soybean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776989/
https://www.ncbi.nlm.nih.gov/pubmed/35069626
http://dx.doi.org/10.3389/fpls.2021.771850
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