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Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives

Androgenesis, which entails cell fate redirection within the microgametophyte, is employed widely for genetic gain in plant breeding programs. Moreover, androgenesis-responsive species provide tractable systems for studying cell cycle regulation, meiotic recombination, and apozygotic embryogenesis w...

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Autores principales: Hale, Brett, Ferrie, Alison M. R., Chellamma, Sreekala, Samuel, J. Pon, Phillips, Gregory C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777211/
https://www.ncbi.nlm.nih.gov/pubmed/35069615
http://dx.doi.org/10.3389/fpls.2021.751230
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author Hale, Brett
Ferrie, Alison M. R.
Chellamma, Sreekala
Samuel, J. Pon
Phillips, Gregory C.
author_facet Hale, Brett
Ferrie, Alison M. R.
Chellamma, Sreekala
Samuel, J. Pon
Phillips, Gregory C.
author_sort Hale, Brett
collection PubMed
description Androgenesis, which entails cell fate redirection within the microgametophyte, is employed widely for genetic gain in plant breeding programs. Moreover, androgenesis-responsive species provide tractable systems for studying cell cycle regulation, meiotic recombination, and apozygotic embryogenesis within plant cells. Past research on androgenesis has focused on protocol development with emphasis on temperature pretreatments of donor plants or floral buds, and tissue culture optimization because androgenesis has different nutritional requirements than somatic embryogenesis. Protocol development for new species and genotypes within responsive species continues to the present day, but slowly. There is more focus presently on understanding how protocols work in order to extend them to additional genotypes and species. Transcriptomic and epigenetic analyses of induced microspores have revealed some of the cellular and molecular responses required for or associated with androgenesis. For example, microRNAs appear to regulate early microspore responses to external stimuli; trichostatin-A, a histone deacetylase inhibitor, acts as an epigenetic additive; ά-phytosulfokine, a five amino acid sulfated peptide, promotes androgenesis in some species. Additionally, present work on gene transfer and genome editing in microspores suggest that future endeavors will likely incorporate greater precision with the genetic composition of microspores used in doubled haploid breeding, thus likely to realize a greater impact on crop improvement. In this review, we evaluate basic breeding applications of androgenesis, explore the utility of genomics and gene editing technologies for protocol development, and provide considerations to overcome genotype specificity and morphogenic recalcitrance in non-model plant systems.
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spelling pubmed-87772112022-01-22 Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives Hale, Brett Ferrie, Alison M. R. Chellamma, Sreekala Samuel, J. Pon Phillips, Gregory C. Front Plant Sci Plant Science Androgenesis, which entails cell fate redirection within the microgametophyte, is employed widely for genetic gain in plant breeding programs. Moreover, androgenesis-responsive species provide tractable systems for studying cell cycle regulation, meiotic recombination, and apozygotic embryogenesis within plant cells. Past research on androgenesis has focused on protocol development with emphasis on temperature pretreatments of donor plants or floral buds, and tissue culture optimization because androgenesis has different nutritional requirements than somatic embryogenesis. Protocol development for new species and genotypes within responsive species continues to the present day, but slowly. There is more focus presently on understanding how protocols work in order to extend them to additional genotypes and species. Transcriptomic and epigenetic analyses of induced microspores have revealed some of the cellular and molecular responses required for or associated with androgenesis. For example, microRNAs appear to regulate early microspore responses to external stimuli; trichostatin-A, a histone deacetylase inhibitor, acts as an epigenetic additive; ά-phytosulfokine, a five amino acid sulfated peptide, promotes androgenesis in some species. Additionally, present work on gene transfer and genome editing in microspores suggest that future endeavors will likely incorporate greater precision with the genetic composition of microspores used in doubled haploid breeding, thus likely to realize a greater impact on crop improvement. In this review, we evaluate basic breeding applications of androgenesis, explore the utility of genomics and gene editing technologies for protocol development, and provide considerations to overcome genotype specificity and morphogenic recalcitrance in non-model plant systems. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8777211/ /pubmed/35069615 http://dx.doi.org/10.3389/fpls.2021.751230 Text en Copyright © 2022 Hale, Ferrie, Chellamma, Samuel and Phillips. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Hale, Brett
Ferrie, Alison M. R.
Chellamma, Sreekala
Samuel, J. Pon
Phillips, Gregory C.
Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives
title Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives
title_full Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives
title_fullStr Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives
title_full_unstemmed Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives
title_short Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives
title_sort androgenesis-based doubled haploidy: past, present, and future perspectives
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777211/
https://www.ncbi.nlm.nih.gov/pubmed/35069615
http://dx.doi.org/10.3389/fpls.2021.751230
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