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Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species

Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gra...

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Autores principales: da Silva, Giarlã Cunha, Gonçalves, Osiel Silva, Rosa, Jéssica Nogueira, França, Kiara Campos, Bossé, Janine Thérèse, Santana, Mateus Ferreira, Langford, Paul Richard, Bazzolli, Denise Mara Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777487/
https://www.ncbi.nlm.nih.gov/pubmed/35069478
http://dx.doi.org/10.3389/fmicb.2021.773284
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author da Silva, Giarlã Cunha
Gonçalves, Osiel Silva
Rosa, Jéssica Nogueira
França, Kiara Campos
Bossé, Janine Thérèse
Santana, Mateus Ferreira
Langford, Paul Richard
Bazzolli, Denise Mara Soares
author_facet da Silva, Giarlã Cunha
Gonçalves, Osiel Silva
Rosa, Jéssica Nogueira
França, Kiara Campos
Bossé, Janine Thérèse
Santana, Mateus Ferreira
Langford, Paul Richard
Bazzolli, Denise Mara Soares
author_sort da Silva, Giarlã Cunha
collection PubMed
description Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gram-negative bacteria including major human and animal pathogens. We generated a comprehensive dataset of the mobilome integrated into genomes, including 10,820 insertion sequences, 2,939 prophages, and 43 integrative and conjugative elements. Also, we assessed plasmid sequences of Pasteurellaceae. Our findings greatly expand the diversity of MGEs for the family, including a description of novel elements. We discovered that MGEs are comparable and dispersed across species and that they also co-occur in genomes, contributing to the family’s ecology via gene transfer. In addition, we investigated the impact of these elements in the dissemination and shaping of AMR genes. A total of 55 different AMR genes were mapped to 721 locations in the dataset. MGEs are linked with 77.6% of AMR genes discovered, indicating their important involvement in the acquisition and transmission of such genes. This study provides an uncharted view of the Pasteurellaceae by demonstrating the global distribution of resistance genes linked with MGEs.
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spelling pubmed-87774872022-01-22 Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species da Silva, Giarlã Cunha Gonçalves, Osiel Silva Rosa, Jéssica Nogueira França, Kiara Campos Bossé, Janine Thérèse Santana, Mateus Ferreira Langford, Paul Richard Bazzolli, Denise Mara Soares Front Microbiol Microbiology Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gram-negative bacteria including major human and animal pathogens. We generated a comprehensive dataset of the mobilome integrated into genomes, including 10,820 insertion sequences, 2,939 prophages, and 43 integrative and conjugative elements. Also, we assessed plasmid sequences of Pasteurellaceae. Our findings greatly expand the diversity of MGEs for the family, including a description of novel elements. We discovered that MGEs are comparable and dispersed across species and that they also co-occur in genomes, contributing to the family’s ecology via gene transfer. In addition, we investigated the impact of these elements in the dissemination and shaping of AMR genes. A total of 55 different AMR genes were mapped to 721 locations in the dataset. MGEs are linked with 77.6% of AMR genes discovered, indicating their important involvement in the acquisition and transmission of such genes. This study provides an uncharted view of the Pasteurellaceae by demonstrating the global distribution of resistance genes linked with MGEs. Frontiers Media S.A. 2022-01-06 /pmc/articles/PMC8777487/ /pubmed/35069478 http://dx.doi.org/10.3389/fmicb.2021.773284 Text en Copyright © 2022 da Silva, Gonçalves, Rosa, França, Bossé, Santana, Langford and Bazzolli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
da Silva, Giarlã Cunha
Gonçalves, Osiel Silva
Rosa, Jéssica Nogueira
França, Kiara Campos
Bossé, Janine Thérèse
Santana, Mateus Ferreira
Langford, Paul Richard
Bazzolli, Denise Mara Soares
Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species
title Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species
title_full Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species
title_fullStr Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species
title_full_unstemmed Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species
title_short Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species
title_sort mobile genetic elements drive antimicrobial resistance gene spread in pasteurellaceae species
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777487/
https://www.ncbi.nlm.nih.gov/pubmed/35069478
http://dx.doi.org/10.3389/fmicb.2021.773284
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