Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan

This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH1...

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Detalles Bibliográficos
Autores principales: Khayrova, Adelya, Lopatin, Sergey, Shagdarova, Balzhima, Sinitsyna, Olga, Sinitsyn, Arkady, Varlamov, Valery
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777618/
https://www.ncbi.nlm.nih.gov/pubmed/35056890
http://dx.doi.org/10.3390/molecules27020577
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author Khayrova, Adelya
Lopatin, Sergey
Shagdarova, Balzhima
Sinitsyna, Olga
Sinitsyn, Arkady
Varlamov, Valery
author_facet Khayrova, Adelya
Lopatin, Sergey
Shagdarova, Balzhima
Sinitsyna, Olga
Sinitsyn, Arkady
Varlamov, Valery
author_sort Khayrova, Adelya
collection PubMed
description This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (M(w)) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of Hermetia illucens as a new source of low molecular weight chitosan with an improved biological activity.
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spelling pubmed-87776182022-01-22 Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan Khayrova, Adelya Lopatin, Sergey Shagdarova, Balzhima Sinitsyna, Olga Sinitsyn, Arkady Varlamov, Valery Molecules Article This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (M(w)) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of Hermetia illucens as a new source of low molecular weight chitosan with an improved biological activity. MDPI 2022-01-17 /pmc/articles/PMC8777618/ /pubmed/35056890 http://dx.doi.org/10.3390/molecules27020577 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khayrova, Adelya
Lopatin, Sergey
Shagdarova, Balzhima
Sinitsyna, Olga
Sinitsyn, Arkady
Varlamov, Valery
Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan
title Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan
title_full Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan
title_fullStr Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan
title_full_unstemmed Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan
title_short Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan
title_sort evaluation of antibacterial and antifungal properties of low molecular weight chitosan extracted from hermetia illucens relative to crab chitosan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777618/
https://www.ncbi.nlm.nih.gov/pubmed/35056890
http://dx.doi.org/10.3390/molecules27020577
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