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The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells

This study aimed to synthesise C(60)–DOX complexes followed by the analysis of their effect on the concentration of metallothionein (MT) as a non-enzymatic antioxidant and on the concentration and activity of superoxide dismutase (SOD) as an antioxidant enzyme in healthy human mammary MCF-10A cells....

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Autores principales: Zaręba, Natalia, Więcławik, Klaudia, Kizek, Rene, Hosnedlova, Bozena, Kepinska, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777724/
https://www.ncbi.nlm.nih.gov/pubmed/35056998
http://dx.doi.org/10.3390/pharmaceutics14010102
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author Zaręba, Natalia
Więcławik, Klaudia
Kizek, Rene
Hosnedlova, Bozena
Kepinska, Marta
author_facet Zaręba, Natalia
Więcławik, Klaudia
Kizek, Rene
Hosnedlova, Bozena
Kepinska, Marta
author_sort Zaręba, Natalia
collection PubMed
description This study aimed to synthesise C(60)–DOX complexes followed by the analysis of their effect on the concentration of metallothionein (MT) as a non-enzymatic antioxidant and on the concentration and activity of superoxide dismutase (SOD) as an antioxidant enzyme in healthy human mammary MCF-10A cells. Dynamic light scattering and electrophoretic light scattering were used to establish the size and zeta potential of the complexes. The MT and SOD concentrations were determined using the ELISA method; SOD activity was determined by tetrazolium salt reduction inhibition. Lower MT concentration following exposure of cells to both DOX and C(60) fullerene compared to the control sample was found. However, the concentration of this protein increased as a consequence of the C(60)–DOX complexes action on MCF-10A cells compared to the control. C(60) used alone did not affect the concentration and activity of SOD in MCF-10A cells. Application of free DOX did not activate cellular antioxidant defence in the form of an increase in SOD concentration or its activity. In contrast treatment of cells with the C(60)–DOX complex resulted in a decrease in SOD1 concentration and a significant increase in SOD activity compared to cells treated with free DOX, C(60) and control. Thus, it was found that C(60)–DOX complexes showed potential for protective effects against DOX-induced toxicity to MCF-10A cells.
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spelling pubmed-87777242022-01-22 The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells Zaręba, Natalia Więcławik, Klaudia Kizek, Rene Hosnedlova, Bozena Kepinska, Marta Pharmaceutics Article This study aimed to synthesise C(60)–DOX complexes followed by the analysis of their effect on the concentration of metallothionein (MT) as a non-enzymatic antioxidant and on the concentration and activity of superoxide dismutase (SOD) as an antioxidant enzyme in healthy human mammary MCF-10A cells. Dynamic light scattering and electrophoretic light scattering were used to establish the size and zeta potential of the complexes. The MT and SOD concentrations were determined using the ELISA method; SOD activity was determined by tetrazolium salt reduction inhibition. Lower MT concentration following exposure of cells to both DOX and C(60) fullerene compared to the control sample was found. However, the concentration of this protein increased as a consequence of the C(60)–DOX complexes action on MCF-10A cells compared to the control. C(60) used alone did not affect the concentration and activity of SOD in MCF-10A cells. Application of free DOX did not activate cellular antioxidant defence in the form of an increase in SOD concentration or its activity. In contrast treatment of cells with the C(60)–DOX complex resulted in a decrease in SOD1 concentration and a significant increase in SOD activity compared to cells treated with free DOX, C(60) and control. Thus, it was found that C(60)–DOX complexes showed potential for protective effects against DOX-induced toxicity to MCF-10A cells. MDPI 2022-01-02 /pmc/articles/PMC8777724/ /pubmed/35056998 http://dx.doi.org/10.3390/pharmaceutics14010102 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zaręba, Natalia
Więcławik, Klaudia
Kizek, Rene
Hosnedlova, Bozena
Kepinska, Marta
The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
title The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
title_full The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
title_fullStr The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
title_full_unstemmed The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
title_short The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
title_sort impact of fullerenes as doxorubicin nano-transporters on metallothionein and superoxide dismutase status in mcf-10a cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777724/
https://www.ncbi.nlm.nih.gov/pubmed/35056998
http://dx.doi.org/10.3390/pharmaceutics14010102
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