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A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso

In this study, cattle farms located in Oudalan and Séno, two provinces in the Sahel region, northern Burkina Faso, were surveyed. Cattle owners were interviewed, cattle were examined for tick infestation, and ticks as well as blood samples were collected during the dry season (October). Blood DNA sa...

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Autores principales: Adjou Moumouni, Paul Franck, Minoungou, Germaine Lim-Bamba, Dovonou, Christian Enonkpon, Galon, Eloiza May, Efstratiou, Artemis, Tumwebaze, Maria Agnes, Byamukama, Benedicto, Vudriko, Patrick, Umemiya-Shirafuji, Rika, Suzuki, Hiroshi, Xuan, Xuenan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777743/
https://www.ncbi.nlm.nih.gov/pubmed/35055979
http://dx.doi.org/10.3390/pathogens11010031
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author Adjou Moumouni, Paul Franck
Minoungou, Germaine Lim-Bamba
Dovonou, Christian Enonkpon
Galon, Eloiza May
Efstratiou, Artemis
Tumwebaze, Maria Agnes
Byamukama, Benedicto
Vudriko, Patrick
Umemiya-Shirafuji, Rika
Suzuki, Hiroshi
Xuan, Xuenan
author_facet Adjou Moumouni, Paul Franck
Minoungou, Germaine Lim-Bamba
Dovonou, Christian Enonkpon
Galon, Eloiza May
Efstratiou, Artemis
Tumwebaze, Maria Agnes
Byamukama, Benedicto
Vudriko, Patrick
Umemiya-Shirafuji, Rika
Suzuki, Hiroshi
Xuan, Xuenan
author_sort Adjou Moumouni, Paul Franck
collection PubMed
description In this study, cattle farms located in Oudalan and Séno, two provinces in the Sahel region, northern Burkina Faso, were surveyed. Cattle owners were interviewed, cattle were examined for tick infestation, and ticks as well as blood samples were collected during the dry season (October). Blood DNA samples were tested for Babesia and Theileria infections using nested PCRs and sequencing. A total of 22 herds, 174 Zebu cattle were investigated at 6 different sites. Overall, 76 cattle (43.7 %) from 18 farms (81.8%) were found infested with ticks. Cattle in Séno, adult cattle (>5 years) and those owned by the Fulani ethnic group were significantly (p < 0.05) more likely to be tick-infested. A total of 144 adult ticks belonging to five species namely: Hyalomma impeltatum, Hyalomma impressum, Hyalomma rufipes, Rhipicephalus evertsi evertsi, and Rhipicephalus guilhoni were collected from the animals. Piroplasms were detected in the blood DNA of 23 (13.2%) cattle. The cattle in Séno and adult cattle were significantly more likely to be piroplasm-positive. Five pathogens diversely distributed were identified. Theileria mutans (12/174), Babesia bigemina (5/174), Theileria annulata (3/174), and Theileria velifera (3/174) were detected for the first time in northern Burkina Faso, whereas Babesia occultans (1/174) was found for the first time in cattle in West Africa. The analysis of the sequences, including B. bigemina RAP-1a, T. annulata Tams1 genes, and the 18S rRNA genes of all the five protozoa, revealed identities ranging from 98.4 to 100% with previously published sequences. Phylogenetic analysis based on the 18S rRNA gene sequences located north Burkina Faso piroplasms in the same clade as isolates from Africa and other regions of the world. Notably, T. mutans sequences were distributed in two clades: the T. mutans Intona strain clade and the Theileria sp. (strain MSD)/ Theileria sp. B15a clade, suggesting the presence of at least two strains in the area. These findings indicate that the control of ticks and tick-borne diseases should be taken into account in strategies to improve animal health in the Sahel region.
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spelling pubmed-87777432022-01-22 A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso Adjou Moumouni, Paul Franck Minoungou, Germaine Lim-Bamba Dovonou, Christian Enonkpon Galon, Eloiza May Efstratiou, Artemis Tumwebaze, Maria Agnes Byamukama, Benedicto Vudriko, Patrick Umemiya-Shirafuji, Rika Suzuki, Hiroshi Xuan, Xuenan Pathogens Article In this study, cattle farms located in Oudalan and Séno, two provinces in the Sahel region, northern Burkina Faso, were surveyed. Cattle owners were interviewed, cattle were examined for tick infestation, and ticks as well as blood samples were collected during the dry season (October). Blood DNA samples were tested for Babesia and Theileria infections using nested PCRs and sequencing. A total of 22 herds, 174 Zebu cattle were investigated at 6 different sites. Overall, 76 cattle (43.7 %) from 18 farms (81.8%) were found infested with ticks. Cattle in Séno, adult cattle (>5 years) and those owned by the Fulani ethnic group were significantly (p < 0.05) more likely to be tick-infested. A total of 144 adult ticks belonging to five species namely: Hyalomma impeltatum, Hyalomma impressum, Hyalomma rufipes, Rhipicephalus evertsi evertsi, and Rhipicephalus guilhoni were collected from the animals. Piroplasms were detected in the blood DNA of 23 (13.2%) cattle. The cattle in Séno and adult cattle were significantly more likely to be piroplasm-positive. Five pathogens diversely distributed were identified. Theileria mutans (12/174), Babesia bigemina (5/174), Theileria annulata (3/174), and Theileria velifera (3/174) were detected for the first time in northern Burkina Faso, whereas Babesia occultans (1/174) was found for the first time in cattle in West Africa. The analysis of the sequences, including B. bigemina RAP-1a, T. annulata Tams1 genes, and the 18S rRNA genes of all the five protozoa, revealed identities ranging from 98.4 to 100% with previously published sequences. Phylogenetic analysis based on the 18S rRNA gene sequences located north Burkina Faso piroplasms in the same clade as isolates from Africa and other regions of the world. Notably, T. mutans sequences were distributed in two clades: the T. mutans Intona strain clade and the Theileria sp. (strain MSD)/ Theileria sp. B15a clade, suggesting the presence of at least two strains in the area. These findings indicate that the control of ticks and tick-borne diseases should be taken into account in strategies to improve animal health in the Sahel region. MDPI 2021-12-28 /pmc/articles/PMC8777743/ /pubmed/35055979 http://dx.doi.org/10.3390/pathogens11010031 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adjou Moumouni, Paul Franck
Minoungou, Germaine Lim-Bamba
Dovonou, Christian Enonkpon
Galon, Eloiza May
Efstratiou, Artemis
Tumwebaze, Maria Agnes
Byamukama, Benedicto
Vudriko, Patrick
Umemiya-Shirafuji, Rika
Suzuki, Hiroshi
Xuan, Xuenan
A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso
title A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso
title_full A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso
title_fullStr A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso
title_full_unstemmed A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso
title_short A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso
title_sort survey of tick infestation and tick-borne piroplasm infection of cattle in oudalan and séno provinces, northern burkina faso
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777743/
https://www.ncbi.nlm.nih.gov/pubmed/35055979
http://dx.doi.org/10.3390/pathogens11010031
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