Cargando…

Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases

Patatin-related phospholipases A (pPLAs) are a group of plant-specific acyl lipid hydrolases that share less homology with phospholipases than that observed in other organisms. Out of the three known subfamilies (pPLAI, pPLAII, and pPLAIII), the pPLAIII member of genes is particularly known for modi...

Descripción completa

Detalles Bibliográficos
Autores principales: Jang, Jin Hoon, Seo, Hae Seong, Lee, Ok Ran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777835/
https://www.ncbi.nlm.nih.gov/pubmed/35050088
http://dx.doi.org/10.3390/plants11020200
_version_ 1784637166984364032
author Jang, Jin Hoon
Seo, Hae Seong
Lee, Ok Ran
author_facet Jang, Jin Hoon
Seo, Hae Seong
Lee, Ok Ran
author_sort Jang, Jin Hoon
collection PubMed
description Patatin-related phospholipases A (pPLAs) are a group of plant-specific acyl lipid hydrolases that share less homology with phospholipases than that observed in other organisms. Out of the three known subfamilies (pPLAI, pPLAII, and pPLAIII), the pPLAIII member of genes is particularly known for modifying the cell wall structure, resulting in less lignin content. Overexpression of pPLAIIIα and ginseng-derived PgpPLAIIIβ in Arabidopsis and hybrid poplar was reported to reduce the lignin content. Lignin is a complex racemic phenolic heteropolymer that forms the key structural material supporting most of the tissues in plants and plays an important role in the adaptive strategies of vascular plants. However, lignin exerts a negative impact on the utilization of plant biomass in the paper and pulp industry, forage digestibility, textile industry, and production of biofuel. Therefore, the overexpression of pPLAIIIγ in Arabidopsis was analyzed in this study. This overexpression led to the formation of dwarf plants with altered anisotropic growth and reduced lignification of the stem. Transcript levels of lignin biosynthesis-related genes, as well as lignin-specific transcription factors, decreased. Peroxidase-mediated oxidation of monolignols occurs in the final stage of lignin polymerization. Two secondary cell wall-specific peroxidases were downregulated following lowered H(2)O(2) levels, which suggests a functional role of peroxidase in the reduction of lignification by pPLAIIIγ when overexpressed in Arabidopsis.
format Online
Article
Text
id pubmed-8777835
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87778352022-01-22 Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases Jang, Jin Hoon Seo, Hae Seong Lee, Ok Ran Plants (Basel) Article Patatin-related phospholipases A (pPLAs) are a group of plant-specific acyl lipid hydrolases that share less homology with phospholipases than that observed in other organisms. Out of the three known subfamilies (pPLAI, pPLAII, and pPLAIII), the pPLAIII member of genes is particularly known for modifying the cell wall structure, resulting in less lignin content. Overexpression of pPLAIIIα and ginseng-derived PgpPLAIIIβ in Arabidopsis and hybrid poplar was reported to reduce the lignin content. Lignin is a complex racemic phenolic heteropolymer that forms the key structural material supporting most of the tissues in plants and plays an important role in the adaptive strategies of vascular plants. However, lignin exerts a negative impact on the utilization of plant biomass in the paper and pulp industry, forage digestibility, textile industry, and production of biofuel. Therefore, the overexpression of pPLAIIIγ in Arabidopsis was analyzed in this study. This overexpression led to the formation of dwarf plants with altered anisotropic growth and reduced lignification of the stem. Transcript levels of lignin biosynthesis-related genes, as well as lignin-specific transcription factors, decreased. Peroxidase-mediated oxidation of monolignols occurs in the final stage of lignin polymerization. Two secondary cell wall-specific peroxidases were downregulated following lowered H(2)O(2) levels, which suggests a functional role of peroxidase in the reduction of lignification by pPLAIIIγ when overexpressed in Arabidopsis. MDPI 2022-01-13 /pmc/articles/PMC8777835/ /pubmed/35050088 http://dx.doi.org/10.3390/plants11020200 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jang, Jin Hoon
Seo, Hae Seong
Lee, Ok Ran
Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases
title Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases
title_full Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases
title_fullStr Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases
title_full_unstemmed Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases
title_short Overexpression of pPLAIIIγ in Arabidopsis Reduced Xylem Lignification of Stem by Regulating Peroxidases
title_sort overexpression of pplaiiiγ in arabidopsis reduced xylem lignification of stem by regulating peroxidases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777835/
https://www.ncbi.nlm.nih.gov/pubmed/35050088
http://dx.doi.org/10.3390/plants11020200
work_keys_str_mv AT jangjinhoon overexpressionofpplaiiiginarabidopsisreducedxylemlignificationofstembyregulatingperoxidases
AT seohaeseong overexpressionofpplaiiiginarabidopsisreducedxylemlignificationofstembyregulatingperoxidases
AT leeokran overexpressionofpplaiiiginarabidopsisreducedxylemlignificationofstembyregulatingperoxidases