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Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array

Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal r...

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Autores principales: Kuo, Ming-Tse, Hsu, Shiuh-Liang, You, Huey-Ling, Kuo, Shu-Fang, Fang, Po-Chiung, Yu, Hun-Ju, Chen, Alexander, Tseng, Chia-Yi, Lai, Yu-Hsuan, Chen, Jiunn-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777873/
https://www.ncbi.nlm.nih.gov/pubmed/35050004
http://dx.doi.org/10.3390/jof8010064
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author Kuo, Ming-Tse
Hsu, Shiuh-Liang
You, Huey-Ling
Kuo, Shu-Fang
Fang, Po-Chiung
Yu, Hun-Ju
Chen, Alexander
Tseng, Chia-Yi
Lai, Yu-Hsuan
Chen, Jiunn-Liang
author_facet Kuo, Ming-Tse
Hsu, Shiuh-Liang
You, Huey-Ling
Kuo, Shu-Fang
Fang, Po-Chiung
Yu, Hun-Ju
Chen, Alexander
Tseng, Chia-Yi
Lai, Yu-Hsuan
Chen, Jiunn-Liang
author_sort Kuo, Ming-Tse
collection PubMed
description Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal regimens. Therefore, a novel dot hybridization array (DHA) was developed to diagnose FK and differentiate Fusarium and Aspergillus keratitis in this study. One hundred forty-six corneal scrapes obtained from one hundred forty-six subjects impressed with clinically suspected FK were used to evaluate the performance of the DHA. Among these patients, 107 (73.3%) patients had actual FK confirmed by culture and DNA sequencing. We found that the DHA had 93.5% sensitivity and 97.4% specificity in diagnosing FK. In addition, this array had 93.2% sensitivity and 93.8% specificity in diagnosing Fusarium keratitis, as well as 83.3% sensitivity and 100% specificity in diagnosing Aspergillus keratitis. Furthermore, it had 83.9% sensitivity and 100% specificity in identifying Fusarium solani keratitis. Thus, this newly developed DHA will be beneficial to earlier diagnosis, more precise treatment, and improve prognosis of FK, by minimizing medical refractory events and surgical needs.
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spelling pubmed-87778732022-01-22 Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array Kuo, Ming-Tse Hsu, Shiuh-Liang You, Huey-Ling Kuo, Shu-Fang Fang, Po-Chiung Yu, Hun-Ju Chen, Alexander Tseng, Chia-Yi Lai, Yu-Hsuan Chen, Jiunn-Liang J Fungi (Basel) Article Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal regimens. Therefore, a novel dot hybridization array (DHA) was developed to diagnose FK and differentiate Fusarium and Aspergillus keratitis in this study. One hundred forty-six corneal scrapes obtained from one hundred forty-six subjects impressed with clinically suspected FK were used to evaluate the performance of the DHA. Among these patients, 107 (73.3%) patients had actual FK confirmed by culture and DNA sequencing. We found that the DHA had 93.5% sensitivity and 97.4% specificity in diagnosing FK. In addition, this array had 93.2% sensitivity and 93.8% specificity in diagnosing Fusarium keratitis, as well as 83.3% sensitivity and 100% specificity in diagnosing Aspergillus keratitis. Furthermore, it had 83.9% sensitivity and 100% specificity in identifying Fusarium solani keratitis. Thus, this newly developed DHA will be beneficial to earlier diagnosis, more precise treatment, and improve prognosis of FK, by minimizing medical refractory events and surgical needs. MDPI 2022-01-07 /pmc/articles/PMC8777873/ /pubmed/35050004 http://dx.doi.org/10.3390/jof8010064 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuo, Ming-Tse
Hsu, Shiuh-Liang
You, Huey-Ling
Kuo, Shu-Fang
Fang, Po-Chiung
Yu, Hun-Ju
Chen, Alexander
Tseng, Chia-Yi
Lai, Yu-Hsuan
Chen, Jiunn-Liang
Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
title Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
title_full Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
title_fullStr Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
title_full_unstemmed Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
title_short Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
title_sort diagnosing fungal keratitis and simultaneously identifying fusarium and aspergillus keratitis with a dot hybridization array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777873/
https://www.ncbi.nlm.nih.gov/pubmed/35050004
http://dx.doi.org/10.3390/jof8010064
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