Cargando…
Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array
Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal r...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777873/ https://www.ncbi.nlm.nih.gov/pubmed/35050004 http://dx.doi.org/10.3390/jof8010064 |
_version_ | 1784637177077956608 |
---|---|
author | Kuo, Ming-Tse Hsu, Shiuh-Liang You, Huey-Ling Kuo, Shu-Fang Fang, Po-Chiung Yu, Hun-Ju Chen, Alexander Tseng, Chia-Yi Lai, Yu-Hsuan Chen, Jiunn-Liang |
author_facet | Kuo, Ming-Tse Hsu, Shiuh-Liang You, Huey-Ling Kuo, Shu-Fang Fang, Po-Chiung Yu, Hun-Ju Chen, Alexander Tseng, Chia-Yi Lai, Yu-Hsuan Chen, Jiunn-Liang |
author_sort | Kuo, Ming-Tse |
collection | PubMed |
description | Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal regimens. Therefore, a novel dot hybridization array (DHA) was developed to diagnose FK and differentiate Fusarium and Aspergillus keratitis in this study. One hundred forty-six corneal scrapes obtained from one hundred forty-six subjects impressed with clinically suspected FK were used to evaluate the performance of the DHA. Among these patients, 107 (73.3%) patients had actual FK confirmed by culture and DNA sequencing. We found that the DHA had 93.5% sensitivity and 97.4% specificity in diagnosing FK. In addition, this array had 93.2% sensitivity and 93.8% specificity in diagnosing Fusarium keratitis, as well as 83.3% sensitivity and 100% specificity in diagnosing Aspergillus keratitis. Furthermore, it had 83.9% sensitivity and 100% specificity in identifying Fusarium solani keratitis. Thus, this newly developed DHA will be beneficial to earlier diagnosis, more precise treatment, and improve prognosis of FK, by minimizing medical refractory events and surgical needs. |
format | Online Article Text |
id | pubmed-8777873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87778732022-01-22 Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array Kuo, Ming-Tse Hsu, Shiuh-Liang You, Huey-Ling Kuo, Shu-Fang Fang, Po-Chiung Yu, Hun-Ju Chen, Alexander Tseng, Chia-Yi Lai, Yu-Hsuan Chen, Jiunn-Liang J Fungi (Basel) Article Fungal keratitis (FK) is one of the most common microbial keratitis, which often leads to poor prognosis as a result of delayed diagnosis. Several studies implied that early differentiation of the two major FK, Fusarium and Aspergillus keratitis, could be helpful in selecting effective anti-fungal regimens. Therefore, a novel dot hybridization array (DHA) was developed to diagnose FK and differentiate Fusarium and Aspergillus keratitis in this study. One hundred forty-six corneal scrapes obtained from one hundred forty-six subjects impressed with clinically suspected FK were used to evaluate the performance of the DHA. Among these patients, 107 (73.3%) patients had actual FK confirmed by culture and DNA sequencing. We found that the DHA had 93.5% sensitivity and 97.4% specificity in diagnosing FK. In addition, this array had 93.2% sensitivity and 93.8% specificity in diagnosing Fusarium keratitis, as well as 83.3% sensitivity and 100% specificity in diagnosing Aspergillus keratitis. Furthermore, it had 83.9% sensitivity and 100% specificity in identifying Fusarium solani keratitis. Thus, this newly developed DHA will be beneficial to earlier diagnosis, more precise treatment, and improve prognosis of FK, by minimizing medical refractory events and surgical needs. MDPI 2022-01-07 /pmc/articles/PMC8777873/ /pubmed/35050004 http://dx.doi.org/10.3390/jof8010064 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kuo, Ming-Tse Hsu, Shiuh-Liang You, Huey-Ling Kuo, Shu-Fang Fang, Po-Chiung Yu, Hun-Ju Chen, Alexander Tseng, Chia-Yi Lai, Yu-Hsuan Chen, Jiunn-Liang Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array |
title | Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array |
title_full | Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array |
title_fullStr | Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array |
title_full_unstemmed | Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array |
title_short | Diagnosing Fungal Keratitis and Simultaneously Identifying Fusarium and Aspergillus Keratitis with a Dot Hybridization Array |
title_sort | diagnosing fungal keratitis and simultaneously identifying fusarium and aspergillus keratitis with a dot hybridization array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777873/ https://www.ncbi.nlm.nih.gov/pubmed/35050004 http://dx.doi.org/10.3390/jof8010064 |
work_keys_str_mv | AT kuomingtse diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT hsushiuhliang diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT youhueyling diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT kuoshufang diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT fangpochiung diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT yuhunju diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT chenalexander diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT tsengchiayi diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT laiyuhsuan diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray AT chenjiunnliang diagnosingfungalkeratitisandsimultaneouslyidentifyingfusariumandaspergilluskeratitiswithadothybridizationarray |