Cargando…
Comparison of Carbonic Anhydrases for CO(2) Sequestration
Strategies for depleting carbon dioxide (CO(2)) from flue gases are urgently needed and carbonic anhydrases (CAs) can contribute to solving this problem. They catalyze the hydration of CO(2) in aqueous solutions and therefore capture the CO(2). However, the harsh conditions due to varying process te...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777876/ https://www.ncbi.nlm.nih.gov/pubmed/35055147 http://dx.doi.org/10.3390/ijms23020957 |
_version_ | 1784637177831882752 |
---|---|
author | Steger, Franziska Reich, Johanna Fuchs, Werner Rittmann, Simon K.-M. R. Gübitz, Georg M. Ribitsch, Doris Bochmann, Günther |
author_facet | Steger, Franziska Reich, Johanna Fuchs, Werner Rittmann, Simon K.-M. R. Gübitz, Georg M. Ribitsch, Doris Bochmann, Günther |
author_sort | Steger, Franziska |
collection | PubMed |
description | Strategies for depleting carbon dioxide (CO(2)) from flue gases are urgently needed and carbonic anhydrases (CAs) can contribute to solving this problem. They catalyze the hydration of CO(2) in aqueous solutions and therefore capture the CO(2). However, the harsh conditions due to varying process temperatures are limiting factors for the application of enzymes. The current study aims to examine four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab) and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA (1814 WAU mg(−1) AwCA) and PmCA (1748 WAU mg(−1) PmCA). AwCA was highly stable in a mesophilic temperature range, whereas PmCA proved to be exceptionally thermostable. Our results indicate the potential to utilize CAs from anaerobic microorganisms to develop CO(2) sequestration applications. |
format | Online Article Text |
id | pubmed-8777876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87778762022-01-22 Comparison of Carbonic Anhydrases for CO(2) Sequestration Steger, Franziska Reich, Johanna Fuchs, Werner Rittmann, Simon K.-M. R. Gübitz, Georg M. Ribitsch, Doris Bochmann, Günther Int J Mol Sci Article Strategies for depleting carbon dioxide (CO(2)) from flue gases are urgently needed and carbonic anhydrases (CAs) can contribute to solving this problem. They catalyze the hydration of CO(2) in aqueous solutions and therefore capture the CO(2). However, the harsh conditions due to varying process temperatures are limiting factors for the application of enzymes. The current study aims to examine four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab) and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA (1814 WAU mg(−1) AwCA) and PmCA (1748 WAU mg(−1) PmCA). AwCA was highly stable in a mesophilic temperature range, whereas PmCA proved to be exceptionally thermostable. Our results indicate the potential to utilize CAs from anaerobic microorganisms to develop CO(2) sequestration applications. MDPI 2022-01-16 /pmc/articles/PMC8777876/ /pubmed/35055147 http://dx.doi.org/10.3390/ijms23020957 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Steger, Franziska Reich, Johanna Fuchs, Werner Rittmann, Simon K.-M. R. Gübitz, Georg M. Ribitsch, Doris Bochmann, Günther Comparison of Carbonic Anhydrases for CO(2) Sequestration |
title | Comparison of Carbonic Anhydrases for CO(2) Sequestration |
title_full | Comparison of Carbonic Anhydrases for CO(2) Sequestration |
title_fullStr | Comparison of Carbonic Anhydrases for CO(2) Sequestration |
title_full_unstemmed | Comparison of Carbonic Anhydrases for CO(2) Sequestration |
title_short | Comparison of Carbonic Anhydrases for CO(2) Sequestration |
title_sort | comparison of carbonic anhydrases for co(2) sequestration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777876/ https://www.ncbi.nlm.nih.gov/pubmed/35055147 http://dx.doi.org/10.3390/ijms23020957 |
work_keys_str_mv | AT stegerfranziska comparisonofcarbonicanhydrasesforco2sequestration AT reichjohanna comparisonofcarbonicanhydrasesforco2sequestration AT fuchswerner comparisonofcarbonicanhydrasesforco2sequestration AT rittmannsimonkmr comparisonofcarbonicanhydrasesforco2sequestration AT gubitzgeorgm comparisonofcarbonicanhydrasesforco2sequestration AT ribitschdoris comparisonofcarbonicanhydrasesforco2sequestration AT bochmanngunther comparisonofcarbonicanhydrasesforco2sequestration |