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Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21

Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolat...

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Autores principales: Li, Yongmei, Xia, Mengyuan, He, Pengbo, Yang, Qiaoming, Wu, Yixin, He, Pengfei, Ahmed, Ayesha, Li, Xiangsong, Wang, Yuehu, Munir, Shahzad, He, Yueqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777893/
https://www.ncbi.nlm.nih.gov/pubmed/35050020
http://dx.doi.org/10.3390/jof8010080
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author Li, Yongmei
Xia, Mengyuan
He, Pengbo
Yang, Qiaoming
Wu, Yixin
He, Pengfei
Ahmed, Ayesha
Li, Xiangsong
Wang, Yuehu
Munir, Shahzad
He, Yueqiu
author_facet Li, Yongmei
Xia, Mengyuan
He, Pengbo
Yang, Qiaoming
Wu, Yixin
He, Pengfei
Ahmed, Ayesha
Li, Xiangsong
Wang, Yuehu
Munir, Shahzad
He, Yueqiu
author_sort Li, Yongmei
collection PubMed
description Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.
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spelling pubmed-87778932022-01-22 Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21 Li, Yongmei Xia, Mengyuan He, Pengbo Yang, Qiaoming Wu, Yixin He, Pengfei Ahmed, Ayesha Li, Xiangsong Wang, Yuehu Munir, Shahzad He, Yueqiu J Fungi (Basel) Article Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit. MDPI 2022-01-14 /pmc/articles/PMC8777893/ /pubmed/35050020 http://dx.doi.org/10.3390/jof8010080 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yongmei
Xia, Mengyuan
He, Pengbo
Yang, Qiaoming
Wu, Yixin
He, Pengfei
Ahmed, Ayesha
Li, Xiangsong
Wang, Yuehu
Munir, Shahzad
He, Yueqiu
Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
title Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
title_full Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
title_fullStr Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
title_full_unstemmed Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
title_short Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21
title_sort developing penicillium digitatum management strategies on post-harvest citrus fruits with metabolic components and colonization of bacillus subtilis l1-21
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777893/
https://www.ncbi.nlm.nih.gov/pubmed/35050020
http://dx.doi.org/10.3390/jof8010080
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