Cargando…

Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017

In this study, we investigated the molecular evolution and phylodynamics of respiratory syncytial virus (RSV) over 10 consecutive seasons (2008–2017) and the genetic variability of the RSV genotypes ON1 and BA in central Taiwan. The ectodomain region of the G gene was sequenced for genotyping. The n...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Chun-Yi, Fang, Yu-Ping, Wang, Li-Chung, Chou, Teh-Ying, Liu, Hsin-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777914/
https://www.ncbi.nlm.nih.gov/pubmed/35062237
http://dx.doi.org/10.3390/v14010032
_version_ 1784637187910795264
author Lee, Chun-Yi
Fang, Yu-Ping
Wang, Li-Chung
Chou, Teh-Ying
Liu, Hsin-Fu
author_facet Lee, Chun-Yi
Fang, Yu-Ping
Wang, Li-Chung
Chou, Teh-Ying
Liu, Hsin-Fu
author_sort Lee, Chun-Yi
collection PubMed
description In this study, we investigated the molecular evolution and phylodynamics of respiratory syncytial virus (RSV) over 10 consecutive seasons (2008–2017) and the genetic variability of the RSV genotypes ON1 and BA in central Taiwan. The ectodomain region of the G gene was sequenced for genotyping. The nucleotide and deduced amino acid sequences of the second hypervariable region of the G protein in RSV ON1 and BA were analyzed. A total of 132 RSV-A and 81 RSV-B isolates were obtained. Phylogenetic analysis revealed that the NA1, ON1, and BA9 genotypes were responsible for the RSV epidemics in central Taiwan in the study period. For RSV-A, the NA1 genotype predominated during the 2008–2011 seasons. The ON1 genotype was first detected in 2011 and replaced NA1 after 2012. For RSV-B, the BA9 and BA10 genotypes cocirculated from 2008 to 2010, but the BA9 genotype has predominated since 2012. Amino acid sequence alignments revealed the continuous evolution of the G gene in the ectodomain region. The predicted N-glycosylation sites were relatively conserved in the ON1 (site 237 and 318) and BA9 (site 296 and 310) genotype strains. Our results contribute to the understanding and prediction of the temporal evolution of RSV at the local level.
format Online
Article
Text
id pubmed-8777914
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87779142022-01-22 Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017 Lee, Chun-Yi Fang, Yu-Ping Wang, Li-Chung Chou, Teh-Ying Liu, Hsin-Fu Viruses Article In this study, we investigated the molecular evolution and phylodynamics of respiratory syncytial virus (RSV) over 10 consecutive seasons (2008–2017) and the genetic variability of the RSV genotypes ON1 and BA in central Taiwan. The ectodomain region of the G gene was sequenced for genotyping. The nucleotide and deduced amino acid sequences of the second hypervariable region of the G protein in RSV ON1 and BA were analyzed. A total of 132 RSV-A and 81 RSV-B isolates were obtained. Phylogenetic analysis revealed that the NA1, ON1, and BA9 genotypes were responsible for the RSV epidemics in central Taiwan in the study period. For RSV-A, the NA1 genotype predominated during the 2008–2011 seasons. The ON1 genotype was first detected in 2011 and replaced NA1 after 2012. For RSV-B, the BA9 and BA10 genotypes cocirculated from 2008 to 2010, but the BA9 genotype has predominated since 2012. Amino acid sequence alignments revealed the continuous evolution of the G gene in the ectodomain region. The predicted N-glycosylation sites were relatively conserved in the ON1 (site 237 and 318) and BA9 (site 296 and 310) genotype strains. Our results contribute to the understanding and prediction of the temporal evolution of RSV at the local level. MDPI 2021-12-24 /pmc/articles/PMC8777914/ /pubmed/35062237 http://dx.doi.org/10.3390/v14010032 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Chun-Yi
Fang, Yu-Ping
Wang, Li-Chung
Chou, Teh-Ying
Liu, Hsin-Fu
Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
title Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
title_full Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
title_fullStr Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
title_full_unstemmed Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
title_short Genetic Diversity and Molecular Epidemiology of Circulating Respiratory Syncytial Virus in Central Taiwan, 2008–2017
title_sort genetic diversity and molecular epidemiology of circulating respiratory syncytial virus in central taiwan, 2008–2017
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777914/
https://www.ncbi.nlm.nih.gov/pubmed/35062237
http://dx.doi.org/10.3390/v14010032
work_keys_str_mv AT leechunyi geneticdiversityandmolecularepidemiologyofcirculatingrespiratorysyncytialvirusincentraltaiwan20082017
AT fangyuping geneticdiversityandmolecularepidemiologyofcirculatingrespiratorysyncytialvirusincentraltaiwan20082017
AT wanglichung geneticdiversityandmolecularepidemiologyofcirculatingrespiratorysyncytialvirusincentraltaiwan20082017
AT choutehying geneticdiversityandmolecularepidemiologyofcirculatingrespiratorysyncytialvirusincentraltaiwan20082017
AT liuhsinfu geneticdiversityandmolecularepidemiologyofcirculatingrespiratorysyncytialvirusincentraltaiwan20082017