Herbivore-Induced Rice Volatiles Attract and Affect the Predation Ability of the Wolf Spiders, Pirata subpiraticus and Pardosa pseudoannulata

SIMPLE SUMMARY: The spiders, Pirata subpiraticus Bösenberg et Strand (Araneae: Lycosidae) and Pardosa pseudoannulata Bösenberg et Strand (Araneae: Lycosidae) are important natural enemies of many rice pests. Herbivore-induced plant volatiles can attract natural enemies to pest locations and are becoming important in integrated pest management. This study assessed the effects of herbivore-induced rice volatiles on the selection behavior, predation ability and field attraction of two species of spiders. The selection frequency of spiders for methyl salicylate, linalool, and 2-heptanone were significantly greater than the blank group. Methyl salicylate can shorten the predatory latency of male P. pseudoannulata and can also trap more P. pseudoannulata in the field. Linalool may also shorten the predatory latency of male P. subpiraticus and increase the daily predation capacity of female P. pseudoannulata. In summary, herbivore-induced rice volatiles attract P. pseudoannulata and P. subpiraticus, and potentially increase their pest control capability. These results provide support for the practical use of herbivore-induced rice volatiles to attract and retain spiders in rice fields. ABSTRACT: Spiders are important natural enemies of rice pests. Studying the effects of herbivore-induced rice volatiles on spider attraction and predation ability may lead to safer methods for pest prevention and control. In this study, four-arm olfactometer, predation ability experiment, and field trapping experiment were used to evaluate the effects of herbivore-induced rice volatiles on Pirata subpiraticus Bösenberg et Strand (Araneae: Lycosidae) and Pardosa pseudoannulata Bösenberg et Strand (Araneae: Lycosidae). The 0.5 μg/μL linalool concentration was attractive, and also shortened the predation latency in male P. subpiraticus and female P. pseudoannulata. The 0.5 μg/μL linalool concentration increased the daily predation capacity of female P. pseudoannulata. Male P. pseudoannulata were attracted to 1.0 g/L methyl salicylate, which also shortened their predation latency. In field experiments, methyl salicylate and linalool were effective for trapping spiders. Herbivore-induced rice volatiles attract rice field spiders and affect their predatory ability. These results suggest that herbivore-induced rice volatiles can be used to attract spiders and provide improved control of rice pests.