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Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †

Ergot alkaloid mycotoxins interfere in many functions associated with serotonergic neurotransmitters. Therefore, the objective was to evaluate whether the association of serotonin (5-hydroxytryptamine, 5-HT) and ergot alkaloids during a 24 h pre-incubation could affect the vascular contractile respo...

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Autores principales: Valente, Eriton E. L., Harmon, David L., Klotz, James L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777993/
https://www.ncbi.nlm.nih.gov/pubmed/35050986
http://dx.doi.org/10.3390/toxins14010009
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author Valente, Eriton E. L.
Harmon, David L.
Klotz, James L.
author_facet Valente, Eriton E. L.
Harmon, David L.
Klotz, James L.
author_sort Valente, Eriton E. L.
collection PubMed
description Ergot alkaloid mycotoxins interfere in many functions associated with serotonergic neurotransmitters. Therefore, the objective was to evaluate whether the association of serotonin (5-hydroxytryptamine, 5-HT) and ergot alkaloids during a 24 h pre-incubation could affect the vascular contractile response to ergot alkaloids. To evaluate the effects of 24 h exposure to 5-HT and ergot alkaloids (ergovaline, ERV), two assays were conducted. The first assay determined the half-maximal inhibitory concentration (IC(50)) following the 24 h pre-exposure period, while the second assay evaluated the effect of IC(50) concentrations of 5-HT and ERV either individually or in combination. There was an interaction between previous exposure to 5-HT and ERV. Previous exposure to 5-HT at the IC(50) concentration of 7.57 × 10(−7) M reduced the contractile response by more than 50% of control, while the exposure to ERV at IC(50) dose of 1.57 × 10(−10) M tended to decrease (p = 0.081) vessel contractility with a response higher than 50% of control. The 24 h previous exposure to both 5-HT and ERV did not potentiate the inhibitory response of blood vessels in comparison with incubation with each compound alone. These results suggest receptor competition between 5-HT and ERV. More studies are necessary to determine the potential of 5-HT to treat toxicosis caused by ergot alkaloids.
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spelling pubmed-87779932022-01-22 Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo † Valente, Eriton E. L. Harmon, David L. Klotz, James L. Toxins (Basel) Article Ergot alkaloid mycotoxins interfere in many functions associated with serotonergic neurotransmitters. Therefore, the objective was to evaluate whether the association of serotonin (5-hydroxytryptamine, 5-HT) and ergot alkaloids during a 24 h pre-incubation could affect the vascular contractile response to ergot alkaloids. To evaluate the effects of 24 h exposure to 5-HT and ergot alkaloids (ergovaline, ERV), two assays were conducted. The first assay determined the half-maximal inhibitory concentration (IC(50)) following the 24 h pre-exposure period, while the second assay evaluated the effect of IC(50) concentrations of 5-HT and ERV either individually or in combination. There was an interaction between previous exposure to 5-HT and ERV. Previous exposure to 5-HT at the IC(50) concentration of 7.57 × 10(−7) M reduced the contractile response by more than 50% of control, while the exposure to ERV at IC(50) dose of 1.57 × 10(−10) M tended to decrease (p = 0.081) vessel contractility with a response higher than 50% of control. The 24 h previous exposure to both 5-HT and ERV did not potentiate the inhibitory response of blood vessels in comparison with incubation with each compound alone. These results suggest receptor competition between 5-HT and ERV. More studies are necessary to determine the potential of 5-HT to treat toxicosis caused by ergot alkaloids. MDPI 2021-12-23 /pmc/articles/PMC8777993/ /pubmed/35050986 http://dx.doi.org/10.3390/toxins14010009 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Valente, Eriton E. L.
Harmon, David L.
Klotz, James L.
Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †
title Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †
title_full Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †
title_fullStr Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †
title_full_unstemmed Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †
title_short Influence of Prolonged Serotonin and Ergovaline Pre-Exposure on Vasoconstriction Ex Vivo †
title_sort influence of prolonged serotonin and ergovaline pre-exposure on vasoconstriction ex vivo †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777993/
https://www.ncbi.nlm.nih.gov/pubmed/35050986
http://dx.doi.org/10.3390/toxins14010009
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