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Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation

This study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were proc...

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Autores principales: Ribeiro, Juliana S., Münchow, Eliseu A., Bordini, Ester A. F., Rodrigues, Nathalie S., Dubey, Nileshkumar, Sasaki, Hajime, Fenno, John C., Schwendeman, Steven, Bottino, Marco C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778034/
https://www.ncbi.nlm.nih.gov/pubmed/35055155
http://dx.doi.org/10.3390/ijms23020971
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author Ribeiro, Juliana S.
Münchow, Eliseu A.
Bordini, Ester A. F.
Rodrigues, Nathalie S.
Dubey, Nileshkumar
Sasaki, Hajime
Fenno, John C.
Schwendeman, Steven
Bottino, Marco C.
author_facet Ribeiro, Juliana S.
Münchow, Eliseu A.
Bordini, Ester A. F.
Rodrigues, Nathalie S.
Dubey, Nileshkumar
Sasaki, Hajime
Fenno, John C.
Schwendeman, Steven
Bottino, Marco C.
author_sort Ribeiro, Juliana S.
collection PubMed
description This study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN- or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN- or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation.
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spelling pubmed-87780342022-01-22 Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation Ribeiro, Juliana S. Münchow, Eliseu A. Bordini, Ester A. F. Rodrigues, Nathalie S. Dubey, Nileshkumar Sasaki, Hajime Fenno, John C. Schwendeman, Steven Bottino, Marco C. Int J Mol Sci Article This study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN- or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN- or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation. MDPI 2022-01-16 /pmc/articles/PMC8778034/ /pubmed/35055155 http://dx.doi.org/10.3390/ijms23020971 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ribeiro, Juliana S.
Münchow, Eliseu A.
Bordini, Ester A. F.
Rodrigues, Nathalie S.
Dubey, Nileshkumar
Sasaki, Hajime
Fenno, John C.
Schwendeman, Steven
Bottino, Marco C.
Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
title Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
title_full Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
title_fullStr Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
title_full_unstemmed Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
title_short Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
title_sort engineering of injectable antibiotic-laden fibrous microparticles gelatin methacryloyl hydrogel for endodontic infection ablation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778034/
https://www.ncbi.nlm.nih.gov/pubmed/35055155
http://dx.doi.org/10.3390/ijms23020971
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