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Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging
Microvesicles, so-called endothelial large extracellular vesicles (LEVs), are of great interest as biological markers and cell-free biotherapies in cardiovascular and oncologic diseases. However, their therapeutic perspectives remain limited due to the lack of reliable data regarding their systemic...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778059/ https://www.ncbi.nlm.nih.gov/pubmed/35057018 http://dx.doi.org/10.3390/pharmaceutics14010121 |
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author | Giraud, Romain Moyon, Anaïs Simoncini, Stéphanie Duchez, Anne-Claire Nail, Vincent Chareyre, Corinne Bouhlel, Ahlem Balasse, Laure Fernandez, Samantha Vallier, Loris Hache, Guillaume Sabatier, Florence Dignat-George, Françoise Lacroix, Romaric Guillet, Benjamin Garrigue, Philippe |
author_facet | Giraud, Romain Moyon, Anaïs Simoncini, Stéphanie Duchez, Anne-Claire Nail, Vincent Chareyre, Corinne Bouhlel, Ahlem Balasse, Laure Fernandez, Samantha Vallier, Loris Hache, Guillaume Sabatier, Florence Dignat-George, Françoise Lacroix, Romaric Guillet, Benjamin Garrigue, Philippe |
author_sort | Giraud, Romain |
collection | PubMed |
description | Microvesicles, so-called endothelial large extracellular vesicles (LEVs), are of great interest as biological markers and cell-free biotherapies in cardiovascular and oncologic diseases. However, their therapeutic perspectives remain limited due to the lack of reliable data regarding their systemic biodistribution after intravenous administration. Methods: Applied to a mouse model of peripheral ischemia, radiolabeled endothelial LEVs were tracked and their in vivo whole-body distribution was quantified by microSPECT/CT imaging. Hindlimb perfusion was followed by LASER Doppler and motility impairment function was evaluated up to day 28 post-ischemia. Results: Early and specific homing of LEVs to ischemic hind limbs was quantified on the day of ischemia and positively correlated with reperfusion intensity at a later stage on day 28 after ischemia, associated with an improved motility function. Conclusions: This concept is a major asset for investigating the biodistribution of LEVs issued from other cell types, including cancer, thus partly contributing to better knowledge and understanding of their fate after injection. |
format | Online Article Text |
id | pubmed-8778059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87780592022-01-22 Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging Giraud, Romain Moyon, Anaïs Simoncini, Stéphanie Duchez, Anne-Claire Nail, Vincent Chareyre, Corinne Bouhlel, Ahlem Balasse, Laure Fernandez, Samantha Vallier, Loris Hache, Guillaume Sabatier, Florence Dignat-George, Françoise Lacroix, Romaric Guillet, Benjamin Garrigue, Philippe Pharmaceutics Article Microvesicles, so-called endothelial large extracellular vesicles (LEVs), are of great interest as biological markers and cell-free biotherapies in cardiovascular and oncologic diseases. However, their therapeutic perspectives remain limited due to the lack of reliable data regarding their systemic biodistribution after intravenous administration. Methods: Applied to a mouse model of peripheral ischemia, radiolabeled endothelial LEVs were tracked and their in vivo whole-body distribution was quantified by microSPECT/CT imaging. Hindlimb perfusion was followed by LASER Doppler and motility impairment function was evaluated up to day 28 post-ischemia. Results: Early and specific homing of LEVs to ischemic hind limbs was quantified on the day of ischemia and positively correlated with reperfusion intensity at a later stage on day 28 after ischemia, associated with an improved motility function. Conclusions: This concept is a major asset for investigating the biodistribution of LEVs issued from other cell types, including cancer, thus partly contributing to better knowledge and understanding of their fate after injection. MDPI 2022-01-04 /pmc/articles/PMC8778059/ /pubmed/35057018 http://dx.doi.org/10.3390/pharmaceutics14010121 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Giraud, Romain Moyon, Anaïs Simoncini, Stéphanie Duchez, Anne-Claire Nail, Vincent Chareyre, Corinne Bouhlel, Ahlem Balasse, Laure Fernandez, Samantha Vallier, Loris Hache, Guillaume Sabatier, Florence Dignat-George, Françoise Lacroix, Romaric Guillet, Benjamin Garrigue, Philippe Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging |
title | Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging |
title_full | Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging |
title_fullStr | Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging |
title_full_unstemmed | Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging |
title_short | Tracking Radiolabeled Endothelial Microvesicles Predicts Their Therapeutic Efficacy: A Proof-of-Concept Study in Peripheral Ischemia Mouse Model Using SPECT/CT Imaging |
title_sort | tracking radiolabeled endothelial microvesicles predicts their therapeutic efficacy: a proof-of-concept study in peripheral ischemia mouse model using spect/ct imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778059/ https://www.ncbi.nlm.nih.gov/pubmed/35057018 http://dx.doi.org/10.3390/pharmaceutics14010121 |
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