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Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment
The storage of plant samples as well as sample preparation for extraction have a significant impact on the profile of metabolites, however, these factors are often overlooked during experiments on vegetables or fruit. It was hypothesized that parameters such as sample storage (freezing) and sample p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778298/ https://www.ncbi.nlm.nih.gov/pubmed/35056815 http://dx.doi.org/10.3390/molecules27020500 |
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author | Wieczorek, Martyna N. Pieczywek, Piotr Mariusz Cybulska, Justyna Zdunek, Artur Jeleń, Henryk H. |
author_facet | Wieczorek, Martyna N. Pieczywek, Piotr Mariusz Cybulska, Justyna Zdunek, Artur Jeleń, Henryk H. |
author_sort | Wieczorek, Martyna N. |
collection | PubMed |
description | The storage of plant samples as well as sample preparation for extraction have a significant impact on the profile of metabolites, however, these factors are often overlooked during experiments on vegetables or fruit. It was hypothesized that parameters such as sample storage (freezing) and sample pre-treatment methods, including the comminution technique or applied enzyme inhibition methods, could significantly influence the extracted volatile metabolome. Significant changes were observed in the volatile profile of broccoli florets frozen in liquid nitrogen at −20 °C. Those differences were mostly related to the concentration of nitriles and aldehydes. Confocal microscopy indicated some tissue deterioration in the case of slow freezing (−20 °C), whereas the structure of tissue, frozen in liquid nitrogen, was practically intact. Myrosinase activity assay proved that the enzyme remains active after freezing. No pH deviation was noted after sample storage - this parameter did not influence the activity of enzymes. Tissue fragmentation and enzyme-inhibition techniques applied prior to the extraction influenced both the qualitative and quantitative composition of the volatile metabolome of broccoli. |
format | Online Article Text |
id | pubmed-8778298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87782982022-01-22 Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment Wieczorek, Martyna N. Pieczywek, Piotr Mariusz Cybulska, Justyna Zdunek, Artur Jeleń, Henryk H. Molecules Article The storage of plant samples as well as sample preparation for extraction have a significant impact on the profile of metabolites, however, these factors are often overlooked during experiments on vegetables or fruit. It was hypothesized that parameters such as sample storage (freezing) and sample pre-treatment methods, including the comminution technique or applied enzyme inhibition methods, could significantly influence the extracted volatile metabolome. Significant changes were observed in the volatile profile of broccoli florets frozen in liquid nitrogen at −20 °C. Those differences were mostly related to the concentration of nitriles and aldehydes. Confocal microscopy indicated some tissue deterioration in the case of slow freezing (−20 °C), whereas the structure of tissue, frozen in liquid nitrogen, was practically intact. Myrosinase activity assay proved that the enzyme remains active after freezing. No pH deviation was noted after sample storage - this parameter did not influence the activity of enzymes. Tissue fragmentation and enzyme-inhibition techniques applied prior to the extraction influenced both the qualitative and quantitative composition of the volatile metabolome of broccoli. MDPI 2022-01-14 /pmc/articles/PMC8778298/ /pubmed/35056815 http://dx.doi.org/10.3390/molecules27020500 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wieczorek, Martyna N. Pieczywek, Piotr Mariusz Cybulska, Justyna Zdunek, Artur Jeleń, Henryk H. Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment |
title | Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment |
title_full | Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment |
title_fullStr | Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment |
title_full_unstemmed | Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment |
title_short | Chemical Changes in the Broccoli Volatilome Depending on the Tissue Treatment |
title_sort | chemical changes in the broccoli volatilome depending on the tissue treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778298/ https://www.ncbi.nlm.nih.gov/pubmed/35056815 http://dx.doi.org/10.3390/molecules27020500 |
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