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NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications

Fibrillar structures derived from plant or animal origin have long been a source of inspiration for the design of new biomaterials. The Asn-Gly-Ile-Trp-Tyr-NH(2) (NGIWY-amide) pentapeptide, isolated from the sea cucumber Apostichopus japonicus, which spontaneously self-assembles in water to form hyd...

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Autores principales: Theodoroula, Nikoleta F., Karavasili, Christina, Vlasiou, Manos C., Primikyri, Alexandra, Nicolaou, Christia, Chatzikonstantinou, Alexandra V., Chatzitaki, Aikaterini-Theodora, Petrou, Christos, Bouropoulos, Nikolaos, Zacharis, Constantinos K., Galatou, Eleftheria, Sarigiannis, Yiannis, Fatouros, Dimitrios G., Vizirianakis, Ioannis S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778326/
https://www.ncbi.nlm.nih.gov/pubmed/35057029
http://dx.doi.org/10.3390/pharmaceutics14010133
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author Theodoroula, Nikoleta F.
Karavasili, Christina
Vlasiou, Manos C.
Primikyri, Alexandra
Nicolaou, Christia
Chatzikonstantinou, Alexandra V.
Chatzitaki, Aikaterini-Theodora
Petrou, Christos
Bouropoulos, Nikolaos
Zacharis, Constantinos K.
Galatou, Eleftheria
Sarigiannis, Yiannis
Fatouros, Dimitrios G.
Vizirianakis, Ioannis S.
author_facet Theodoroula, Nikoleta F.
Karavasili, Christina
Vlasiou, Manos C.
Primikyri, Alexandra
Nicolaou, Christia
Chatzikonstantinou, Alexandra V.
Chatzitaki, Aikaterini-Theodora
Petrou, Christos
Bouropoulos, Nikolaos
Zacharis, Constantinos K.
Galatou, Eleftheria
Sarigiannis, Yiannis
Fatouros, Dimitrios G.
Vizirianakis, Ioannis S.
author_sort Theodoroula, Nikoleta F.
collection PubMed
description Fibrillar structures derived from plant or animal origin have long been a source of inspiration for the design of new biomaterials. The Asn-Gly-Ile-Trp-Tyr-NH(2) (NGIWY-amide) pentapeptide, isolated from the sea cucumber Apostichopus japonicus, which spontaneously self-assembles in water to form hydrogel, pertains to this category. In this study, we evaluated this ultra-short cosmetic bioinspired peptide as vector for local drug delivery applications. Combining nuclear magnetic resonance, circular dichroism, infrared spectroscopy, X-ray diffraction, and rheological studies, the synthesized pentapeptide formed a stiff hydrogel with a high β-sheet content. Molecular dynamic simulations aligned well with scanning electron and atomic-force microscopy studies, revealing a highly filamentous structure with the fibers adopting a helical-twisted morphology. Model dye localization within the supramolecular hydrogel provided insights on the preferential distribution of hydrophobic and hydrophilic compounds in the hydrogel network. That was further depicted in the diffusion kinetics of drugs differing in their aqueous solubility and molecular weight, namely, doxorubicin hydrochloride, curcumin, and octreotide acetate, highlighting its versatility as a delivery vector of both hydrophobic and hydrophilic compounds of different molecular weight. Along with the observed cytocompatibility of the hydrogel, the NGIWY-amide pentapeptide may offer new approaches for cell growth, drug delivery, and 3D bioprinting tissue-engineering applications.
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spelling pubmed-87783262022-01-22 NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications Theodoroula, Nikoleta F. Karavasili, Christina Vlasiou, Manos C. Primikyri, Alexandra Nicolaou, Christia Chatzikonstantinou, Alexandra V. Chatzitaki, Aikaterini-Theodora Petrou, Christos Bouropoulos, Nikolaos Zacharis, Constantinos K. Galatou, Eleftheria Sarigiannis, Yiannis Fatouros, Dimitrios G. Vizirianakis, Ioannis S. Pharmaceutics Article Fibrillar structures derived from plant or animal origin have long been a source of inspiration for the design of new biomaterials. The Asn-Gly-Ile-Trp-Tyr-NH(2) (NGIWY-amide) pentapeptide, isolated from the sea cucumber Apostichopus japonicus, which spontaneously self-assembles in water to form hydrogel, pertains to this category. In this study, we evaluated this ultra-short cosmetic bioinspired peptide as vector for local drug delivery applications. Combining nuclear magnetic resonance, circular dichroism, infrared spectroscopy, X-ray diffraction, and rheological studies, the synthesized pentapeptide formed a stiff hydrogel with a high β-sheet content. Molecular dynamic simulations aligned well with scanning electron and atomic-force microscopy studies, revealing a highly filamentous structure with the fibers adopting a helical-twisted morphology. Model dye localization within the supramolecular hydrogel provided insights on the preferential distribution of hydrophobic and hydrophilic compounds in the hydrogel network. That was further depicted in the diffusion kinetics of drugs differing in their aqueous solubility and molecular weight, namely, doxorubicin hydrochloride, curcumin, and octreotide acetate, highlighting its versatility as a delivery vector of both hydrophobic and hydrophilic compounds of different molecular weight. Along with the observed cytocompatibility of the hydrogel, the NGIWY-amide pentapeptide may offer new approaches for cell growth, drug delivery, and 3D bioprinting tissue-engineering applications. MDPI 2022-01-06 /pmc/articles/PMC8778326/ /pubmed/35057029 http://dx.doi.org/10.3390/pharmaceutics14010133 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Theodoroula, Nikoleta F.
Karavasili, Christina
Vlasiou, Manos C.
Primikyri, Alexandra
Nicolaou, Christia
Chatzikonstantinou, Alexandra V.
Chatzitaki, Aikaterini-Theodora
Petrou, Christos
Bouropoulos, Nikolaos
Zacharis, Constantinos K.
Galatou, Eleftheria
Sarigiannis, Yiannis
Fatouros, Dimitrios G.
Vizirianakis, Ioannis S.
NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications
title NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications
title_full NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications
title_fullStr NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications
title_full_unstemmed NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications
title_short NGIWY-Amide: A Bioinspired Ultrashort Self-Assembled Peptide Gelator for Local Drug Delivery Applications
title_sort ngiwy-amide: a bioinspired ultrashort self-assembled peptide gelator for local drug delivery applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778326/
https://www.ncbi.nlm.nih.gov/pubmed/35057029
http://dx.doi.org/10.3390/pharmaceutics14010133
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