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Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778389/ https://www.ncbi.nlm.nih.gov/pubmed/35050085 http://dx.doi.org/10.3390/plants11020197 |
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author | Zharova, Daria A. Ivanova, Alexandra N. Drozdova, Irina V. Belyaeva, Alla I. Boldina, Olga N. Voitsekhovskaja, Olga V. Tyutereva, Elena V. |
author_facet | Zharova, Daria A. Ivanova, Alexandra N. Drozdova, Irina V. Belyaeva, Alla I. Boldina, Olga N. Voitsekhovskaja, Olga V. Tyutereva, Elena V. |
author_sort | Zharova, Daria A. |
collection | PubMed |
description | The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however, after a switch to stressful conditions, H. lacustris experiences massive cell death which negatively influences astaxanthin yield. This study addressed the possibility to improve cell survival in H. lacustris subjected to salinity via manipulation of the levels of autophagy using AZD8055, a known inhibitor of TOR kinase previously shown to accelerate autophagy in several microalgae. Addition of NaCl in concentrations of 0.2% or 0.8% to the growth medium induced formation of autophagosomes in H. lacustris, while simultaneous addition of AZD8055 up to a final concentration of 0.2 µM further stimulated this process. AZD8055 significantly improved the yield of H. lacustris cells after 5 days of exposure to 0.2% NaCl. Strikingly, this occurred by acceleration of cell growth, and not by acceleration of aplanospore formation. The level of astaxanthin synthesis was not affected by AZD8055. However, cytological data suggested a role of autophagosomes, lysosomes and Golgi cisternae in cell remodeling during high salt stress. |
format | Online Article Text |
id | pubmed-8778389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87783892022-01-22 Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity Zharova, Daria A. Ivanova, Alexandra N. Drozdova, Irina V. Belyaeva, Alla I. Boldina, Olga N. Voitsekhovskaja, Olga V. Tyutereva, Elena V. Plants (Basel) Article The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however, after a switch to stressful conditions, H. lacustris experiences massive cell death which negatively influences astaxanthin yield. This study addressed the possibility to improve cell survival in H. lacustris subjected to salinity via manipulation of the levels of autophagy using AZD8055, a known inhibitor of TOR kinase previously shown to accelerate autophagy in several microalgae. Addition of NaCl in concentrations of 0.2% or 0.8% to the growth medium induced formation of autophagosomes in H. lacustris, while simultaneous addition of AZD8055 up to a final concentration of 0.2 µM further stimulated this process. AZD8055 significantly improved the yield of H. lacustris cells after 5 days of exposure to 0.2% NaCl. Strikingly, this occurred by acceleration of cell growth, and not by acceleration of aplanospore formation. The level of astaxanthin synthesis was not affected by AZD8055. However, cytological data suggested a role of autophagosomes, lysosomes and Golgi cisternae in cell remodeling during high salt stress. MDPI 2022-01-12 /pmc/articles/PMC8778389/ /pubmed/35050085 http://dx.doi.org/10.3390/plants11020197 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zharova, Daria A. Ivanova, Alexandra N. Drozdova, Irina V. Belyaeva, Alla I. Boldina, Olga N. Voitsekhovskaja, Olga V. Tyutereva, Elena V. Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity |
title | Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity |
title_full | Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity |
title_fullStr | Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity |
title_full_unstemmed | Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity |
title_short | Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity |
title_sort | role of autophagy in haematococcus lacustris cell growth under salinity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778389/ https://www.ncbi.nlm.nih.gov/pubmed/35050085 http://dx.doi.org/10.3390/plants11020197 |
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