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Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity

The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however...

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Autores principales: Zharova, Daria A., Ivanova, Alexandra N., Drozdova, Irina V., Belyaeva, Alla I., Boldina, Olga N., Voitsekhovskaja, Olga V., Tyutereva, Elena V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778389/
https://www.ncbi.nlm.nih.gov/pubmed/35050085
http://dx.doi.org/10.3390/plants11020197
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author Zharova, Daria A.
Ivanova, Alexandra N.
Drozdova, Irina V.
Belyaeva, Alla I.
Boldina, Olga N.
Voitsekhovskaja, Olga V.
Tyutereva, Elena V.
author_facet Zharova, Daria A.
Ivanova, Alexandra N.
Drozdova, Irina V.
Belyaeva, Alla I.
Boldina, Olga N.
Voitsekhovskaja, Olga V.
Tyutereva, Elena V.
author_sort Zharova, Daria A.
collection PubMed
description The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however, after a switch to stressful conditions, H. lacustris experiences massive cell death which negatively influences astaxanthin yield. This study addressed the possibility to improve cell survival in H. lacustris subjected to salinity via manipulation of the levels of autophagy using AZD8055, a known inhibitor of TOR kinase previously shown to accelerate autophagy in several microalgae. Addition of NaCl in concentrations of 0.2% or 0.8% to the growth medium induced formation of autophagosomes in H. lacustris, while simultaneous addition of AZD8055 up to a final concentration of 0.2 µM further stimulated this process. AZD8055 significantly improved the yield of H. lacustris cells after 5 days of exposure to 0.2% NaCl. Strikingly, this occurred by acceleration of cell growth, and not by acceleration of aplanospore formation. The level of astaxanthin synthesis was not affected by AZD8055. However, cytological data suggested a role of autophagosomes, lysosomes and Golgi cisternae in cell remodeling during high salt stress.
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spelling pubmed-87783892022-01-22 Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity Zharova, Daria A. Ivanova, Alexandra N. Drozdova, Irina V. Belyaeva, Alla I. Boldina, Olga N. Voitsekhovskaja, Olga V. Tyutereva, Elena V. Plants (Basel) Article The microalga Haematococcus lacustris (formerly H. pluvialis) is able to accumulate high amounts of the carotenoid astaxanthin in the course of adaptation to stresses like salinity. Technologies aimed at production of natural astaxanthin for commercial purposes often involve salinity stress; however, after a switch to stressful conditions, H. lacustris experiences massive cell death which negatively influences astaxanthin yield. This study addressed the possibility to improve cell survival in H. lacustris subjected to salinity via manipulation of the levels of autophagy using AZD8055, a known inhibitor of TOR kinase previously shown to accelerate autophagy in several microalgae. Addition of NaCl in concentrations of 0.2% or 0.8% to the growth medium induced formation of autophagosomes in H. lacustris, while simultaneous addition of AZD8055 up to a final concentration of 0.2 µM further stimulated this process. AZD8055 significantly improved the yield of H. lacustris cells after 5 days of exposure to 0.2% NaCl. Strikingly, this occurred by acceleration of cell growth, and not by acceleration of aplanospore formation. The level of astaxanthin synthesis was not affected by AZD8055. However, cytological data suggested a role of autophagosomes, lysosomes and Golgi cisternae in cell remodeling during high salt stress. MDPI 2022-01-12 /pmc/articles/PMC8778389/ /pubmed/35050085 http://dx.doi.org/10.3390/plants11020197 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zharova, Daria A.
Ivanova, Alexandra N.
Drozdova, Irina V.
Belyaeva, Alla I.
Boldina, Olga N.
Voitsekhovskaja, Olga V.
Tyutereva, Elena V.
Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
title Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
title_full Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
title_fullStr Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
title_full_unstemmed Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
title_short Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity
title_sort role of autophagy in haematococcus lacustris cell growth under salinity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778389/
https://www.ncbi.nlm.nih.gov/pubmed/35050085
http://dx.doi.org/10.3390/plants11020197
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