Cargando…

Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research

The treatment of uveal melanoma and its metastases has not evolved sufficiently over the last decades in comparison to other tumour entities, posing a great challenge in the field of ocular oncology. Despite improvements in the conventional treatment regime and new discoveries about the genetic and...

Descripción completa

Detalles Bibliográficos
Autores principales: Sokolenko, Ekaterina A., Berchner-Pfannschmidt, Utta, Ting, Saskia C., Schmid, Kurt W., Bechrakis, Nikolaos E., Seitz, Berthold, Tsimpaki, Theodora, Kraemer, Miriam Monika, Fiorentzis, Miltiadis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778438/
https://www.ncbi.nlm.nih.gov/pubmed/35056909
http://dx.doi.org/10.3390/pharmaceutics14010013
_version_ 1784637324416516096
author Sokolenko, Ekaterina A.
Berchner-Pfannschmidt, Utta
Ting, Saskia C.
Schmid, Kurt W.
Bechrakis, Nikolaos E.
Seitz, Berthold
Tsimpaki, Theodora
Kraemer, Miriam Monika
Fiorentzis, Miltiadis
author_facet Sokolenko, Ekaterina A.
Berchner-Pfannschmidt, Utta
Ting, Saskia C.
Schmid, Kurt W.
Bechrakis, Nikolaos E.
Seitz, Berthold
Tsimpaki, Theodora
Kraemer, Miriam Monika
Fiorentzis, Miltiadis
author_sort Sokolenko, Ekaterina A.
collection PubMed
description The treatment of uveal melanoma and its metastases has not evolved sufficiently over the last decades in comparison to other tumour entities, posing a great challenge in the field of ocular oncology. Despite improvements in the conventional treatment regime and new discoveries about the genetic and molecular background of the primary tumour, effective treatment strategies to either prevent tumours or treat patients with advanced or metastatic disease are still lacking. New therapeutic options are necessary in order to achieve satisfactory local tumour control, reduce the risk of metastasis development, and preserve the eyeball and possibly the visual function of the eye. The development of in vivo model systems remains crucial for the identification and investigation of potential novel treatment modalities. The aim of this study was the optimisation of the chorioallantoic membrane (CAM) model for uveal melanoma research. We analysed the established CAM assay and its modification after the implantation of three-dimensional spheroids. The chorioallantoic membrane of a chick embryo was used to implant uveal melanoma-cell-line-derived spheroids in order to study their growth rate, angiogenic potential, and metastatic capability. Using the UM 92.1, UPMD2, UPMM3, and Mel270 cell lines, we were able to improve the viability of the embryos from 20% to >80% and to achieve up to a fourfold volume increase of the transplanted spheroid masses. The results point to the value of an optimised chicken embryo assay as an in vivo model for testing novel therapies for uveal melanoma by simplifying the research conditions and by contributing to a considerable reduction in animal experiments.
format Online
Article
Text
id pubmed-8778438
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87784382022-01-22 Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research Sokolenko, Ekaterina A. Berchner-Pfannschmidt, Utta Ting, Saskia C. Schmid, Kurt W. Bechrakis, Nikolaos E. Seitz, Berthold Tsimpaki, Theodora Kraemer, Miriam Monika Fiorentzis, Miltiadis Pharmaceutics Article The treatment of uveal melanoma and its metastases has not evolved sufficiently over the last decades in comparison to other tumour entities, posing a great challenge in the field of ocular oncology. Despite improvements in the conventional treatment regime and new discoveries about the genetic and molecular background of the primary tumour, effective treatment strategies to either prevent tumours or treat patients with advanced or metastatic disease are still lacking. New therapeutic options are necessary in order to achieve satisfactory local tumour control, reduce the risk of metastasis development, and preserve the eyeball and possibly the visual function of the eye. The development of in vivo model systems remains crucial for the identification and investigation of potential novel treatment modalities. The aim of this study was the optimisation of the chorioallantoic membrane (CAM) model for uveal melanoma research. We analysed the established CAM assay and its modification after the implantation of three-dimensional spheroids. The chorioallantoic membrane of a chick embryo was used to implant uveal melanoma-cell-line-derived spheroids in order to study their growth rate, angiogenic potential, and metastatic capability. Using the UM 92.1, UPMD2, UPMM3, and Mel270 cell lines, we were able to improve the viability of the embryos from 20% to >80% and to achieve up to a fourfold volume increase of the transplanted spheroid masses. The results point to the value of an optimised chicken embryo assay as an in vivo model for testing novel therapies for uveal melanoma by simplifying the research conditions and by contributing to a considerable reduction in animal experiments. MDPI 2021-12-22 /pmc/articles/PMC8778438/ /pubmed/35056909 http://dx.doi.org/10.3390/pharmaceutics14010013 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sokolenko, Ekaterina A.
Berchner-Pfannschmidt, Utta
Ting, Saskia C.
Schmid, Kurt W.
Bechrakis, Nikolaos E.
Seitz, Berthold
Tsimpaki, Theodora
Kraemer, Miriam Monika
Fiorentzis, Miltiadis
Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research
title Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research
title_full Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research
title_fullStr Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research
title_full_unstemmed Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research
title_short Optimisation of the Chicken Chorioallantoic Membrane Assay in Uveal Melanoma Research
title_sort optimisation of the chicken chorioallantoic membrane assay in uveal melanoma research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778438/
https://www.ncbi.nlm.nih.gov/pubmed/35056909
http://dx.doi.org/10.3390/pharmaceutics14010013
work_keys_str_mv AT sokolenkoekaterinaa optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT berchnerpfannschmidtutta optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT tingsaskiac optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT schmidkurtw optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT bechrakisnikolaose optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT seitzberthold optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT tsimpakitheodora optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT kraemermiriammonika optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch
AT fiorentzismiltiadis optimisationofthechickenchorioallantoicmembraneassayinuvealmelanomaresearch