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Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms

Background: Henoch-Schönlein purpura, now called immunoglobulin A (IgA) vasculitis, is a common autoimmune disease in children, its association with gut microbiota composition remains unknown. Methods: The collected cases were divided into three groups: G1 group of simple skin type, G2 group with no...

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Detalles Bibliográficos
Autores principales: Li, Meng, Wang, Xiaoming, Lin, Xingjie, Bian, Xiuju, Jing, Rui, Frelinger, Andrew, Zhang, Aijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778574/
https://www.ncbi.nlm.nih.gov/pubmed/35071141
http://dx.doi.org/10.3389/fped.2021.800677
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author Li, Meng
Wang, Xiaoming
Lin, Xingjie
Bian, Xiuju
Jing, Rui
Frelinger, Andrew
Zhang, Aijun
author_facet Li, Meng
Wang, Xiaoming
Lin, Xingjie
Bian, Xiuju
Jing, Rui
Frelinger, Andrew
Zhang, Aijun
author_sort Li, Meng
collection PubMed
description Background: Henoch-Schönlein purpura, now called immunoglobulin A (IgA) vasculitis, is a common autoimmune disease in children, its association with gut microbiota composition remains unknown. Methods: The collected cases were divided into three groups: G1 group of simple skin type, G2 group with no digestive tract expression, G3 group of mixed digestive tract, and C group of healthy children. The fecal samples of each group of children were collected and the sequencing data was processed and analyzed. The dilution curve reflected the reasonableness of the amount of sequencing data. Results: The number of species composition sequences in the G1, G2 and G3 groups was lower than that in the C group, especially for the G2 and G3 groups. The four most abundant bacteria were Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria. The relative abundance of Proteobacteria in the G2 and G3 groups was significantly higher than that in the G1 and C groups, while the relative abundance of Actinobacteria was significantly reduced, and the relative abundance of Actinobacteria in the G1 group was lower than that in the C group. Principal component analysis of the UPGMA clustering tree and each group of samples showed that the microbial community composition of the same group of samples was similar. Conclusions: The abundance of intestinal microbes in children with IgA vasculitis is lower than in normal children. Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria are the four most abundant bacteria in the intestinal flora of children. Proteobacteria and Actinobacteria are associated with organ involvement in IgA vasculitis.
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spelling pubmed-87785742022-01-22 Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms Li, Meng Wang, Xiaoming Lin, Xingjie Bian, Xiuju Jing, Rui Frelinger, Andrew Zhang, Aijun Front Pediatr Pediatrics Background: Henoch-Schönlein purpura, now called immunoglobulin A (IgA) vasculitis, is a common autoimmune disease in children, its association with gut microbiota composition remains unknown. Methods: The collected cases were divided into three groups: G1 group of simple skin type, G2 group with no digestive tract expression, G3 group of mixed digestive tract, and C group of healthy children. The fecal samples of each group of children were collected and the sequencing data was processed and analyzed. The dilution curve reflected the reasonableness of the amount of sequencing data. Results: The number of species composition sequences in the G1, G2 and G3 groups was lower than that in the C group, especially for the G2 and G3 groups. The four most abundant bacteria were Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria. The relative abundance of Proteobacteria in the G2 and G3 groups was significantly higher than that in the G1 and C groups, while the relative abundance of Actinobacteria was significantly reduced, and the relative abundance of Actinobacteria in the G1 group was lower than that in the C group. Principal component analysis of the UPGMA clustering tree and each group of samples showed that the microbial community composition of the same group of samples was similar. Conclusions: The abundance of intestinal microbes in children with IgA vasculitis is lower than in normal children. Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria are the four most abundant bacteria in the intestinal flora of children. Proteobacteria and Actinobacteria are associated with organ involvement in IgA vasculitis. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8778574/ /pubmed/35071141 http://dx.doi.org/10.3389/fped.2021.800677 Text en Copyright © 2022 Li, Wang, Lin, Bian, Jing, Frelinger and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Li, Meng
Wang, Xiaoming
Lin, Xingjie
Bian, Xiuju
Jing, Rui
Frelinger, Andrew
Zhang, Aijun
Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms
title Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms
title_full Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms
title_fullStr Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms
title_full_unstemmed Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms
title_short Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms
title_sort comparison and analysis of gut microbiota in children with iga vasculitis with different clinical symptoms
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778574/
https://www.ncbi.nlm.nih.gov/pubmed/35071141
http://dx.doi.org/10.3389/fped.2021.800677
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