Chinese Cabbage Changes Its Release of Volatiles to Defend against Spodoptera litura

SIMPLE SUMMARY: Biological control is an important direction for pest control in the future, and chemical ecology is an indispensable part of biological control. Therefore, we tested the selection of Spodoptera litura and parasitic wasps on the volatiles of different treatments of cabbage and collected and analyzed the volatiles of different treatments of cabbage. This study found that cabbage was fed by Spodoptera litura to produce volatiles to avoid Spodoptera litura while also attracting Microplitis similis. As a result, some compounds were found to be related to the behavior of Spodoptera litura and Microplitis similis. These results provide a theoretical basis for searching for biological control resources and chemical control. ABSTRACT: Plants respond to herbivorous insect attacks by releasing volatiles that directly harm the herbivore or that indirectly harm the herbivore by attracting its natural enemies. Although the larvae of Spodoptera litura (the tobacco cutworm) are known to induce the release of host plant volatiles, the effects of such volatiles on host location by S. litura and by the parasitoid Microplitis similis, a natural enemy of S. litura larvae, are poorly understood. Here, we found that both the regurgitate of S. litura larvae and S. litura-infested cabbage leaves attracted M. similis. S. litura had a reduced preference for cabbage plants that had been infested with S. litura for 24 or 48 h. M. similis selection of plants was positively correlated with the release of limonene; linalool and hexadecane, and was negatively correlated with the release of (E)-2-hexenal and 1-Butene, 4-isothiocyanato. S. litura selection of plants was positively correlated with the release of (E)-2-hexenal, 1-Butene, 4-isothiocyanato, and decanal, and was negatively correlated with the release of limonene, nonanal, hexadecane, heptadecane, and octadecane. Our results indicate that host plant volatiles can regulate the behavior of S. litura and M. similis.