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Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics

Advances in cancer research over the past half-century have clearly determined the molecular origins of the disease. Central to the use of molecular signatures for continued progress, including rapid, reliable, and early diagnosis is the use of biomarkers. Specifically, extracellular vesicles as bio...

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Autores principales: Singh, Premanshu Kumar, Patel, Aarti, Kaffenes, Anastasia, Hord, Catherine, Kesterson, Delaney, Prakash, Shaurya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778688/
https://www.ncbi.nlm.nih.gov/pubmed/35056304
http://dx.doi.org/10.3390/mi13010139
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author Singh, Premanshu Kumar
Patel, Aarti
Kaffenes, Anastasia
Hord, Catherine
Kesterson, Delaney
Prakash, Shaurya
author_facet Singh, Premanshu Kumar
Patel, Aarti
Kaffenes, Anastasia
Hord, Catherine
Kesterson, Delaney
Prakash, Shaurya
author_sort Singh, Premanshu Kumar
collection PubMed
description Advances in cancer research over the past half-century have clearly determined the molecular origins of the disease. Central to the use of molecular signatures for continued progress, including rapid, reliable, and early diagnosis is the use of biomarkers. Specifically, extracellular vesicles as biomarker cargo holders have generated significant interest. However, the isolation, purification, and subsequent analysis of these extracellular vesicles remain a challenge. Technological advances driven by microfluidics-enabled devices have made the challenges for isolation of extracellular vesicles an emerging area of research with significant possibilities for use in clinical settings enabling point-of-care diagnostics for cancer. In this article, we present a tutorial review of the existing microfluidic technologies for cancer diagnostics with a focus on extracellular vesicle isolation methods.
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spelling pubmed-87786882022-01-22 Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics Singh, Premanshu Kumar Patel, Aarti Kaffenes, Anastasia Hord, Catherine Kesterson, Delaney Prakash, Shaurya Micromachines (Basel) Review Advances in cancer research over the past half-century have clearly determined the molecular origins of the disease. Central to the use of molecular signatures for continued progress, including rapid, reliable, and early diagnosis is the use of biomarkers. Specifically, extracellular vesicles as biomarker cargo holders have generated significant interest. However, the isolation, purification, and subsequent analysis of these extracellular vesicles remain a challenge. Technological advances driven by microfluidics-enabled devices have made the challenges for isolation of extracellular vesicles an emerging area of research with significant possibilities for use in clinical settings enabling point-of-care diagnostics for cancer. In this article, we present a tutorial review of the existing microfluidic technologies for cancer diagnostics with a focus on extracellular vesicle isolation methods. MDPI 2022-01-16 /pmc/articles/PMC8778688/ /pubmed/35056304 http://dx.doi.org/10.3390/mi13010139 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Singh, Premanshu Kumar
Patel, Aarti
Kaffenes, Anastasia
Hord, Catherine
Kesterson, Delaney
Prakash, Shaurya
Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
title Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
title_full Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
title_fullStr Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
title_full_unstemmed Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
title_short Microfluidic Approaches and Methods Enabling Extracellular Vesicle Isolation for Cancer Diagnostics
title_sort microfluidic approaches and methods enabling extracellular vesicle isolation for cancer diagnostics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778688/
https://www.ncbi.nlm.nih.gov/pubmed/35056304
http://dx.doi.org/10.3390/mi13010139
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