Cargando…

The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production

Background: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (ASMT)-overexpressed melatonin...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Hao, Cui, Xudai, Guan, Shengyu, Li, Guangdong, Yao, Yujun, Wu, Haixin, Zhang, Jinlong, Zhang, Xiaosheng, Yu, Tuan, Li, Yunxiang, Lian, Zhengxing, Zhang, Lu, Liu, Guoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778916/
https://www.ncbi.nlm.nih.gov/pubmed/35056885
http://dx.doi.org/10.3390/molecules27020572
_version_ 1784637446224347136
author Wu, Hao
Cui, Xudai
Guan, Shengyu
Li, Guangdong
Yao, Yujun
Wu, Haixin
Zhang, Jinlong
Zhang, Xiaosheng
Yu, Tuan
Li, Yunxiang
Lian, Zhengxing
Zhang, Lu
Liu, Guoshi
author_facet Wu, Hao
Cui, Xudai
Guan, Shengyu
Li, Guangdong
Yao, Yujun
Wu, Haixin
Zhang, Jinlong
Zhang, Xiaosheng
Yu, Tuan
Li, Yunxiang
Lian, Zhengxing
Zhang, Lu
Liu, Guoshi
author_sort Wu, Hao
collection PubMed
description Background: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (ASMT)-overexpressed melatonin-enriched dairy goats were successfully generated through the use of pBC1-ASMT expression vector construction and prokaryotic embryo microinjection. Results: These transgenic goats have the same normal phenotype as the wild-type goats (WT). However, the melatonin levels in their blood and milk were significantly increased (p < 0.05). In addition, the quality of their milk was also improved, showing elevated protein content and a reduced somatic cell number compared to the WT goats. No significant changes were detected in the intestinal microbiota patterns between groups. When the animals were challenged by the intravenous injection of E. coli, the ASMT-overexpressed goats had a lower level of pro-inflammatory cytokines and higher anti-inflammatory cytokines compared to the WT goats. Metabolic analysis uncovered a unique arachidonic acid metabolism pattern in transgenic goats. Conclusions: The increased melatonin production due to ASMT overexpression in the transgenic goats may have contributed to their improved milk quality and enhanced the anti-inflammatory ability compared to the WT goats.
format Online
Article
Text
id pubmed-8778916
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87789162022-01-22 The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production Wu, Hao Cui, Xudai Guan, Shengyu Li, Guangdong Yao, Yujun Wu, Haixin Zhang, Jinlong Zhang, Xiaosheng Yu, Tuan Li, Yunxiang Lian, Zhengxing Zhang, Lu Liu, Guoshi Molecules Article Background: Transgenic animal production is an important means of livestock breeding and can be used to model pharmaceutical applications. Methods: In this study, to explore the biological activity of endogenously produced melatonin, Acetylserotonin-O-methyltransferase (ASMT)-overexpressed melatonin-enriched dairy goats were successfully generated through the use of pBC1-ASMT expression vector construction and prokaryotic embryo microinjection. Results: These transgenic goats have the same normal phenotype as the wild-type goats (WT). However, the melatonin levels in their blood and milk were significantly increased (p < 0.05). In addition, the quality of their milk was also improved, showing elevated protein content and a reduced somatic cell number compared to the WT goats. No significant changes were detected in the intestinal microbiota patterns between groups. When the animals were challenged by the intravenous injection of E. coli, the ASMT-overexpressed goats had a lower level of pro-inflammatory cytokines and higher anti-inflammatory cytokines compared to the WT goats. Metabolic analysis uncovered a unique arachidonic acid metabolism pattern in transgenic goats. Conclusions: The increased melatonin production due to ASMT overexpression in the transgenic goats may have contributed to their improved milk quality and enhanced the anti-inflammatory ability compared to the WT goats. MDPI 2022-01-17 /pmc/articles/PMC8778916/ /pubmed/35056885 http://dx.doi.org/10.3390/molecules27020572 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Hao
Cui, Xudai
Guan, Shengyu
Li, Guangdong
Yao, Yujun
Wu, Haixin
Zhang, Jinlong
Zhang, Xiaosheng
Yu, Tuan
Li, Yunxiang
Lian, Zhengxing
Zhang, Lu
Liu, Guoshi
The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
title The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
title_full The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
title_fullStr The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
title_full_unstemmed The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
title_short The Improved Milk Quality and Enhanced Anti-Inflammatory Effect in Acetylserotonin-O-methyltransferase (ASMT) Overexpressed Goats: An Association with the Elevated Endogenous Melatonin Production
title_sort improved milk quality and enhanced anti-inflammatory effect in acetylserotonin-o-methyltransferase (asmt) overexpressed goats: an association with the elevated endogenous melatonin production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778916/
https://www.ncbi.nlm.nih.gov/pubmed/35056885
http://dx.doi.org/10.3390/molecules27020572
work_keys_str_mv AT wuhao theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT cuixudai theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT guanshengyu theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT liguangdong theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT yaoyujun theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT wuhaixin theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT zhangjinlong theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT zhangxiaosheng theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT yutuan theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT liyunxiang theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT lianzhengxing theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT zhanglu theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT liuguoshi theimprovedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT wuhao improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT cuixudai improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT guanshengyu improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT liguangdong improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT yaoyujun improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT wuhaixin improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT zhangjinlong improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT zhangxiaosheng improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT yutuan improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT liyunxiang improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT lianzhengxing improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT zhanglu improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction
AT liuguoshi improvedmilkqualityandenhancedantiinflammatoryeffectinacetylserotoninomethyltransferaseasmtoverexpressedgoatsanassociationwiththeelevatedendogenousmelatoninproduction