Cargando…
A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection
Background and Objectives: Wound healing (WH) is a complex natural process: the achieving of a proper WH with standard therapies sometimes is not fulfilled and it is often observed in aged and diabetic patients, leading to intractable ulcers. In recent years, autologous micrograft (AMG) therapies ha...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779014/ https://www.ncbi.nlm.nih.gov/pubmed/35056381 http://dx.doi.org/10.3390/medicina58010073 |
_version_ | 1784637467239907328 |
---|---|
author | Niimi, Yuta Baba, Kyoko Tsuchida, Masako Takeda, Akira |
author_facet | Niimi, Yuta Baba, Kyoko Tsuchida, Masako Takeda, Akira |
author_sort | Niimi, Yuta |
collection | PubMed |
description | Background and Objectives: Wound healing (WH) is a complex natural process: the achieving of a proper WH with standard therapies sometimes is not fulfilled and it is often observed in aged and diabetic patients, leading to intractable ulcers. In recent years, autologous micrograft (AMG) therapies have become a new, effective, and affordable wound care strategy among both researchers and clinicians. In this study, a 72-year-old female patient underwent a combination of treatments using micrograft and negative pressure wound therapy (NPWT) on a postoperative skin ulcer after a benign tumor resection on the back with the aim to present an innovative method to treat skin ulceration using AMG combined with an artificial dermal scaffold and NPWT. Materials and Methods: A section of the artificial dermal scaffold, infused with micrografts, was sampled prior to transplant, and sections were collected postoperatively on days 3 and 7. Hematoxylin-eosin (HE) and immunohistochemical stains were employed for the evaluation of Cytokeratin AE1/AE3, desmin, and Factor VIII. Additionally, on postoperative day 3, NPWT dressing was evaluated using HE stains, as well. The resulting HE and immunostaining analysis revealed red blood cells and tissue fragments within the collagen layers of the artificial dermis prior to transplant. On postoperative day 3, collagen layers of the artificial dermis revealed red blood cells and neutrophils based on HE stains, and scattering of cytokeratin AE1/AE3-positive cells were detected by immunostaining. The HE stains on postoperative day 7 showed more red blood cells and neutrophils within the collagen layers of the artificial dermis than on day 3, an increase in cytokeratin AE1/AE3-positive cells, and tissue stained positively with desmin and Factor VIII. Results: Results suggest that the effects of both micrografts and migratory cells have likely accelerated the wound healing process. Furthermore, the NPWT dressing on day 3 showed almost no cells within the dressing. This indicated that restarting NPWT therapy immediately after micrograft transplant did not draw out cells within the scaffold. Conclusions: Micrograft treatment and NPWT may serve to be a useful combination therapy for complex processes of wound healing. |
format | Online Article Text |
id | pubmed-8779014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87790142022-01-22 A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection Niimi, Yuta Baba, Kyoko Tsuchida, Masako Takeda, Akira Medicina (Kaunas) Case Report Background and Objectives: Wound healing (WH) is a complex natural process: the achieving of a proper WH with standard therapies sometimes is not fulfilled and it is often observed in aged and diabetic patients, leading to intractable ulcers. In recent years, autologous micrograft (AMG) therapies have become a new, effective, and affordable wound care strategy among both researchers and clinicians. In this study, a 72-year-old female patient underwent a combination of treatments using micrograft and negative pressure wound therapy (NPWT) on a postoperative skin ulcer after a benign tumor resection on the back with the aim to present an innovative method to treat skin ulceration using AMG combined with an artificial dermal scaffold and NPWT. Materials and Methods: A section of the artificial dermal scaffold, infused with micrografts, was sampled prior to transplant, and sections were collected postoperatively on days 3 and 7. Hematoxylin-eosin (HE) and immunohistochemical stains were employed for the evaluation of Cytokeratin AE1/AE3, desmin, and Factor VIII. Additionally, on postoperative day 3, NPWT dressing was evaluated using HE stains, as well. The resulting HE and immunostaining analysis revealed red blood cells and tissue fragments within the collagen layers of the artificial dermis prior to transplant. On postoperative day 3, collagen layers of the artificial dermis revealed red blood cells and neutrophils based on HE stains, and scattering of cytokeratin AE1/AE3-positive cells were detected by immunostaining. The HE stains on postoperative day 7 showed more red blood cells and neutrophils within the collagen layers of the artificial dermis than on day 3, an increase in cytokeratin AE1/AE3-positive cells, and tissue stained positively with desmin and Factor VIII. Results: Results suggest that the effects of both micrografts and migratory cells have likely accelerated the wound healing process. Furthermore, the NPWT dressing on day 3 showed almost no cells within the dressing. This indicated that restarting NPWT therapy immediately after micrograft transplant did not draw out cells within the scaffold. Conclusions: Micrograft treatment and NPWT may serve to be a useful combination therapy for complex processes of wound healing. MDPI 2022-01-04 /pmc/articles/PMC8779014/ /pubmed/35056381 http://dx.doi.org/10.3390/medicina58010073 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Case Report Niimi, Yuta Baba, Kyoko Tsuchida, Masako Takeda, Akira A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection |
title | A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection |
title_full | A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection |
title_fullStr | A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection |
title_full_unstemmed | A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection |
title_short | A Histological Evaluation of Artificial Dermal Scaffold Used in Micrograft Treatment: A Case Study of Micrograft and NPWT Performed on a Postoperative Ulcer Formation after Tumor Resection |
title_sort | histological evaluation of artificial dermal scaffold used in micrograft treatment: a case study of micrograft and npwt performed on a postoperative ulcer formation after tumor resection |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779014/ https://www.ncbi.nlm.nih.gov/pubmed/35056381 http://dx.doi.org/10.3390/medicina58010073 |
work_keys_str_mv | AT niimiyuta ahistologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT babakyoko ahistologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT tsuchidamasako ahistologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT takedaakira ahistologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT niimiyuta histologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT babakyoko histologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT tsuchidamasako histologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection AT takedaakira histologicalevaluationofartificialdermalscaffoldusedinmicrografttreatmentacasestudyofmicrograftandnpwtperformedonapostoperativeulcerformationaftertumorresection |