Cargando…

Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes

Characterization of the bovine leukocyte antigen (BoLA) DRB3 gene has shown that specific alleles associate with susceptibility or resilience to the progression of bovine leukemia virus (BLV), measured by proviral load (PVL). Through surveillance of multi-farm BLV eradication field trials, we observ...

Descripción completa

Detalles Bibliográficos
Autores principales: Lohr, Chaelynne E., Sporer, Kelly R. B., Brigham, Kelsey A., Pavliscak, Laura A., Mason, Matelyn M., Borgman, Andrew, Ruggiero, Vickie J., Taxis, Tasia M., Bartlett, Paul C., Droscha, Casey J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779071/
https://www.ncbi.nlm.nih.gov/pubmed/35056052
http://dx.doi.org/10.3390/pathogens11010104
_version_ 1784637481197502464
author Lohr, Chaelynne E.
Sporer, Kelly R. B.
Brigham, Kelsey A.
Pavliscak, Laura A.
Mason, Matelyn M.
Borgman, Andrew
Ruggiero, Vickie J.
Taxis, Tasia M.
Bartlett, Paul C.
Droscha, Casey J.
author_facet Lohr, Chaelynne E.
Sporer, Kelly R. B.
Brigham, Kelsey A.
Pavliscak, Laura A.
Mason, Matelyn M.
Borgman, Andrew
Ruggiero, Vickie J.
Taxis, Tasia M.
Bartlett, Paul C.
Droscha, Casey J.
author_sort Lohr, Chaelynne E.
collection PubMed
description Characterization of the bovine leukocyte antigen (BoLA) DRB3 gene has shown that specific alleles associate with susceptibility or resilience to the progression of bovine leukemia virus (BLV), measured by proviral load (PVL). Through surveillance of multi-farm BLV eradication field trials, we observed differential phenotypes within seropositive cows that persist from months to years. We sought to develop a multiplex next-generation sequencing workflow (NGS-SBT) capable of genotyping 384 samples per run to assess the relationship between BLV phenotype and two BoLA genes. We utilized longitudinal results from milk ELISA screening and subsequent blood collections on seropositive cows for PVL determination using a novel BLV proviral load multiplex qPCR assay to phenotype the cows. Repeated diagnostic observations defined two distinct phenotypes in our study population, ELISA-positive cows that do not harbor detectable levels of provirus and those who do have persistent proviral loads. In total, 565 cows from nine Midwest dairy farms were selected for NGS-SBT, with 558 cows: 168 BLV susceptible (ELISA-positive/PVL-positive) and 390 BLV resilient (ELISA-positive/PVL-negative) successfully genotyped. Three BoLA-DRB3 alleles, including one novel allele, were shown to associate with disease resilience, *009:02, *044:01, and *048:02 were found at rates of 97.5%, 86.5%, and 90.3%, respectively, within the phenotypically resilient population. Alternatively, DRB3*015:01 and *027:03, both known to associate with disease progression, were found at rates of 81.1% and 92.3%, respectively, within the susceptible population. This study helps solidify the immunogenetic relationship between BoLA-DRB3 alleles and BLV infection status of these two phenotypic groupings of US dairy cattle.
format Online
Article
Text
id pubmed-8779071
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87790712022-01-22 Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes Lohr, Chaelynne E. Sporer, Kelly R. B. Brigham, Kelsey A. Pavliscak, Laura A. Mason, Matelyn M. Borgman, Andrew Ruggiero, Vickie J. Taxis, Tasia M. Bartlett, Paul C. Droscha, Casey J. Pathogens Article Characterization of the bovine leukocyte antigen (BoLA) DRB3 gene has shown that specific alleles associate with susceptibility or resilience to the progression of bovine leukemia virus (BLV), measured by proviral load (PVL). Through surveillance of multi-farm BLV eradication field trials, we observed differential phenotypes within seropositive cows that persist from months to years. We sought to develop a multiplex next-generation sequencing workflow (NGS-SBT) capable of genotyping 384 samples per run to assess the relationship between BLV phenotype and two BoLA genes. We utilized longitudinal results from milk ELISA screening and subsequent blood collections on seropositive cows for PVL determination using a novel BLV proviral load multiplex qPCR assay to phenotype the cows. Repeated diagnostic observations defined two distinct phenotypes in our study population, ELISA-positive cows that do not harbor detectable levels of provirus and those who do have persistent proviral loads. In total, 565 cows from nine Midwest dairy farms were selected for NGS-SBT, with 558 cows: 168 BLV susceptible (ELISA-positive/PVL-positive) and 390 BLV resilient (ELISA-positive/PVL-negative) successfully genotyped. Three BoLA-DRB3 alleles, including one novel allele, were shown to associate with disease resilience, *009:02, *044:01, and *048:02 were found at rates of 97.5%, 86.5%, and 90.3%, respectively, within the phenotypically resilient population. Alternatively, DRB3*015:01 and *027:03, both known to associate with disease progression, were found at rates of 81.1% and 92.3%, respectively, within the susceptible population. This study helps solidify the immunogenetic relationship between BoLA-DRB3 alleles and BLV infection status of these two phenotypic groupings of US dairy cattle. MDPI 2022-01-15 /pmc/articles/PMC8779071/ /pubmed/35056052 http://dx.doi.org/10.3390/pathogens11010104 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lohr, Chaelynne E.
Sporer, Kelly R. B.
Brigham, Kelsey A.
Pavliscak, Laura A.
Mason, Matelyn M.
Borgman, Andrew
Ruggiero, Vickie J.
Taxis, Tasia M.
Bartlett, Paul C.
Droscha, Casey J.
Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes
title Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes
title_full Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes
title_fullStr Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes
title_full_unstemmed Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes
title_short Phenotypic Selection of Dairy Cattle Infected with Bovine Leukemia Virus Demonstrates Immunogenetic Resilience through NGS-Based Genotyping of BoLA MHC Class II Genes
title_sort phenotypic selection of dairy cattle infected with bovine leukemia virus demonstrates immunogenetic resilience through ngs-based genotyping of bola mhc class ii genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779071/
https://www.ncbi.nlm.nih.gov/pubmed/35056052
http://dx.doi.org/10.3390/pathogens11010104
work_keys_str_mv AT lohrchaelynnee phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT sporerkellyrb phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT brighamkelseya phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT pavliscaklauraa phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT masonmatelynm phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT borgmanandrew phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT ruggierovickiej phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT taxistasiam phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT bartlettpaulc phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes
AT droschacaseyj phenotypicselectionofdairycattleinfectedwithbovineleukemiavirusdemonstratesimmunogeneticresiliencethroughngsbasedgenotypingofbolamhcclassiigenes